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Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)

Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quant...

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Autores principales: Kyriakou, Sotiris, Michailidou, Kyriaki, Amery, Tom, Stewart, Kyle, Winyard, Paul G., Trafalis, Dimitrios T., Franco, Rodrigo, Pappa, Aglaia, Panayiotidis, Mihalis I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706002/
https://www.ncbi.nlm.nih.gov/pubmed/36457522
http://dx.doi.org/10.3389/fpls.2022.998755
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author Kyriakou, Sotiris
Michailidou, Kyriaki
Amery, Tom
Stewart, Kyle
Winyard, Paul G.
Trafalis, Dimitrios T.
Franco, Rodrigo
Pappa, Aglaia
Panayiotidis, Mihalis I.
author_facet Kyriakou, Sotiris
Michailidou, Kyriaki
Amery, Tom
Stewart, Kyle
Winyard, Paul G.
Trafalis, Dimitrios T.
Franco, Rodrigo
Pappa, Aglaia
Panayiotidis, Mihalis I.
author_sort Kyriakou, Sotiris
collection PubMed
description Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quantification. In doing so, we have utilized reproducible analytical methodologies based on liquid chromatography with tandem mass spectrometry by either positive or negative ion mode. Due to the instability and volatility of isothiocyanates, we followed an ammonia derivatization protocol which converts them into respective ionizable thiourea derivatives. The analytes’ content distribution map was created on watercress flowers, leaves and stems. We have demonstrated that watercress contains significantly higher levels of gluconasturtiin, phenethyl isothiocyanate, quercetin-3-O-rutinoside and isorhamnetin, among others, with their content decreasing from flowers (82.11 ± 0.63, 273.89 ± 0.88, 1459.30 ± 12.95 and 289.40 ± 1.37 ng/g of dry extract respectively) to leaves (32.25 ± 0.74, 125.02 ± 0.52, 1197.86 ± 4.24 and 196.47 ± 3.65 ng/g of det extract respectively) to stems (9.20 ± 0.11, 64.7 ± 0.9, 41.02 ± 0.18, 65.67 ± 0.84 ng/g of dry extract respectivbely). Pearson’s correlation analysis has shown that the content of isothiocyanates doesn’t depend only on the bioconversion of individual glucosinolates but also on other glucosinolates of the same group. Overall, we have provided comprehensive analytical data of the major watercress metabolites thereby providing an opportunity to exploit different parts of watercress for potential therapeutic applications.
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spelling pubmed-97060022022-11-30 Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress) Kyriakou, Sotiris Michailidou, Kyriaki Amery, Tom Stewart, Kyle Winyard, Paul G. Trafalis, Dimitrios T. Franco, Rodrigo Pappa, Aglaia Panayiotidis, Mihalis I. Front Plant Sci Plant Science Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quantification. In doing so, we have utilized reproducible analytical methodologies based on liquid chromatography with tandem mass spectrometry by either positive or negative ion mode. Due to the instability and volatility of isothiocyanates, we followed an ammonia derivatization protocol which converts them into respective ionizable thiourea derivatives. The analytes’ content distribution map was created on watercress flowers, leaves and stems. We have demonstrated that watercress contains significantly higher levels of gluconasturtiin, phenethyl isothiocyanate, quercetin-3-O-rutinoside and isorhamnetin, among others, with their content decreasing from flowers (82.11 ± 0.63, 273.89 ± 0.88, 1459.30 ± 12.95 and 289.40 ± 1.37 ng/g of dry extract respectively) to leaves (32.25 ± 0.74, 125.02 ± 0.52, 1197.86 ± 4.24 and 196.47 ± 3.65 ng/g of det extract respectively) to stems (9.20 ± 0.11, 64.7 ± 0.9, 41.02 ± 0.18, 65.67 ± 0.84 ng/g of dry extract respectivbely). Pearson’s correlation analysis has shown that the content of isothiocyanates doesn’t depend only on the bioconversion of individual glucosinolates but also on other glucosinolates of the same group. Overall, we have provided comprehensive analytical data of the major watercress metabolites thereby providing an opportunity to exploit different parts of watercress for potential therapeutic applications. Frontiers Media S.A. 2022-11-15 /pmc/articles/PMC9706002/ /pubmed/36457522 http://dx.doi.org/10.3389/fpls.2022.998755 Text en Copyright © 2022 Kyriakou, Michailidou, Amery, Stewart, Winyard, Trafalis, Franco, Pappa and Panayiotidis https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kyriakou, Sotiris
Michailidou, Kyriaki
Amery, Tom
Stewart, Kyle
Winyard, Paul G.
Trafalis, Dimitrios T.
Franco, Rodrigo
Pappa, Aglaia
Panayiotidis, Mihalis I.
Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title_full Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title_fullStr Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title_full_unstemmed Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title_short Polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of Nasturtium officinale (Watercress)
title_sort polyphenolics, glucosinolates and isothiocyanates profiling of aerial parts of nasturtium officinale (watercress)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706002/
https://www.ncbi.nlm.nih.gov/pubmed/36457522
http://dx.doi.org/10.3389/fpls.2022.998755
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