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Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously
In recent years, clinical cases of inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) have been emerging and increasing in chicken flocks worldwide. Mixed infections with 2 or more fowl adenovirus (FAdV) serotypes were common in these cases. Herein, we collected a clinical sample tha...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706605/ https://www.ncbi.nlm.nih.gov/pubmed/36436374 http://dx.doi.org/10.1016/j.psj.2022.102295 |
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author | Cui, Ning Lu, Mei Sun, Shiping Sun, Shouli Xu, Chuantian Su, Shuai Hrabchenko, Nataliia Huang, Qinghua |
author_facet | Cui, Ning Lu, Mei Sun, Shiping Sun, Shouli Xu, Chuantian Su, Shuai Hrabchenko, Nataliia Huang, Qinghua |
author_sort | Cui, Ning |
collection | PubMed |
description | In recent years, clinical cases of inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) have been emerging and increasing in chicken flocks worldwide. Mixed infections with 2 or more fowl adenovirus (FAdV) serotypes were common in these cases. Herein, we collected a clinical sample that was positive for FAdV from 40-day-old broilers with IBH and HPS symptoms in Shandong province of China and determined the complete genome of FAdVs on the Illumina HiSeq4000 platform. The results showed that the sample contained 2 FAdV strains of D species and C species and named SD1763-1 and SD1763-2 respectively. The genome of SD1763-1 strain was 43,913 nt in length, with a G+C content of 53.51%, whereas SD1763-2 strain was 43,721 nt in length, with a G+C content of 54.87%. Sequence alignment and phylogenetic analysis revealed that strain SD1763-1 was clustered together with serotype 2/11 of FAdV-D, and SD1763-2 was clustered together with FAdV-4. There is no recombination between the genomes of the 2 viruses of FAdV-D and FAdV-C in the present study. This is the first report of obtaining 2 genomic sequences of FAdV strains simultaneously by direct use of deep sequencing in one clinical individual chicken sample, which provided direct evidence for mixed infections of adenovirus serotypes in the clinic and enriched the genome data to explore the geographic biomarkers and virulence signatures of the genus Aviadenovirus. |
format | Online Article Text |
id | pubmed-9706605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97066052022-11-30 Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously Cui, Ning Lu, Mei Sun, Shiping Sun, Shouli Xu, Chuantian Su, Shuai Hrabchenko, Nataliia Huang, Qinghua Poult Sci IMMUNOLOGY, HEALTH AND DISEASE In recent years, clinical cases of inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) have been emerging and increasing in chicken flocks worldwide. Mixed infections with 2 or more fowl adenovirus (FAdV) serotypes were common in these cases. Herein, we collected a clinical sample that was positive for FAdV from 40-day-old broilers with IBH and HPS symptoms in Shandong province of China and determined the complete genome of FAdVs on the Illumina HiSeq4000 platform. The results showed that the sample contained 2 FAdV strains of D species and C species and named SD1763-1 and SD1763-2 respectively. The genome of SD1763-1 strain was 43,913 nt in length, with a G+C content of 53.51%, whereas SD1763-2 strain was 43,721 nt in length, with a G+C content of 54.87%. Sequence alignment and phylogenetic analysis revealed that strain SD1763-1 was clustered together with serotype 2/11 of FAdV-D, and SD1763-2 was clustered together with FAdV-4. There is no recombination between the genomes of the 2 viruses of FAdV-D and FAdV-C in the present study. This is the first report of obtaining 2 genomic sequences of FAdV strains simultaneously by direct use of deep sequencing in one clinical individual chicken sample, which provided direct evidence for mixed infections of adenovirus serotypes in the clinic and enriched the genome data to explore the geographic biomarkers and virulence signatures of the genus Aviadenovirus. Elsevier 2022-10-29 /pmc/articles/PMC9706605/ /pubmed/36436374 http://dx.doi.org/10.1016/j.psj.2022.102295 Text en © 2022 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | IMMUNOLOGY, HEALTH AND DISEASE Cui, Ning Lu, Mei Sun, Shiping Sun, Shouli Xu, Chuantian Su, Shuai Hrabchenko, Nataliia Huang, Qinghua Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title | Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title_full | Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title_fullStr | Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title_full_unstemmed | Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title_short | Illumina high-throughput sequencing for the genome of emerging fowl adenovirus D species and C species simultaneously |
title_sort | illumina high-throughput sequencing for the genome of emerging fowl adenovirus d species and c species simultaneously |
topic | IMMUNOLOGY, HEALTH AND DISEASE |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706605/ https://www.ncbi.nlm.nih.gov/pubmed/36436374 http://dx.doi.org/10.1016/j.psj.2022.102295 |
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