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Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods

The current study aimed to evaluate the changes in lipid accumulation and oxidative status in pigeon crops during different breeding stages. Forty-two pairs of adult pigeons were randomly assigned to 7 groups. Lipid droplet accumulation in pigeon crops was visualized by using oil red O staining from...

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Detalles Bibliográficos
Autores principales: Xie, P., Zhu, J.G., Wang, L.X., Liu, Y., Diao, E.J., Gong, D.Q., Liu, T.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706646/
https://www.ncbi.nlm.nih.gov/pubmed/36436376
http://dx.doi.org/10.1016/j.psj.2022.102289
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author Xie, P.
Zhu, J.G.
Wang, L.X.
Liu, Y.
Diao, E.J.
Gong, D.Q.
Liu, T.W.
author_facet Xie, P.
Zhu, J.G.
Wang, L.X.
Liu, Y.
Diao, E.J.
Gong, D.Q.
Liu, T.W.
author_sort Xie, P.
collection PubMed
description The current study aimed to evaluate the changes in lipid accumulation and oxidative status in pigeon crops during different breeding stages. Forty-two pairs of adult pigeons were randomly assigned to 7 groups. Lipid droplet accumulation in pigeon crops was visualized by using oil red O staining from d 17 of incubation (I17) to d 7 of chick rearing (R7). Transmission electron microscopy analysis showed swollen mitochondria with disintegration of cristae and typical characteristics of endoplasmic reticulum stress in crop tissues at R1 compared with those at I4. During the peak of pigeon milk formation, the concentrations of reactive oxygen species, and oxidative damage markers (advanced oxidation protein products, 8-hydroxy-2 deoxyguanosine, and malondialdehyde) and the enzyme activities of total superoxide dismutase and glutathione peroxidase were all elevated significantly (P < 0.05). The protein concentration of B-cell lymphoma-2 associated X in crop tissues was significantly higher at R1, while the level of B-cell lymphoma-2 protein in males was the highest at I4 (P < 0.05). The ratio of B-cell lymphoma-2 associated X protein (Bax)/B-cell lymphoma-2 (Bcl-2) in both male and female crops peaked at R1 (P < 0.05). Gene expression of the key enzymes involved in mitochondrial and peroxisomal fatty acid β-oxidation was investigated in crops. In males, the gene expression of carnitine palmitoyltransferase 1a peaked at R15, and that of carnitine palmitoyltransferase 2 increased significantly from R1 to R15 (P < 0.05). The mRNA abundance of long chain 3-hydroxyacyl-CoA dehydrogenase increased to the maximum value at R1 and I17 in males and females, respectively. From I17 to R7, the mRNA levels of acyl-CoA oxidase 1 and acyl-CoA oxidase 2 were decreased in pigeon crops (P < 0.05). Conclusively, lipid droplet accumulation was found in male and female pigeon crops from the end of incubation to the early stage of chick rearing. Although antioxidant defence and mitochondrial fatty acid β-oxidation were both mobilized, oxidative stress in crop tissues still occurred during the peak of milk formation.
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spelling pubmed-97066462022-11-30 Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods Xie, P. Zhu, J.G. Wang, L.X. Liu, Y. Diao, E.J. Gong, D.Q. Liu, T.W. Poult Sci PHYSIOLOGY AND REPRODUCTION The current study aimed to evaluate the changes in lipid accumulation and oxidative status in pigeon crops during different breeding stages. Forty-two pairs of adult pigeons were randomly assigned to 7 groups. Lipid droplet accumulation in pigeon crops was visualized by using oil red O staining from d 17 of incubation (I17) to d 7 of chick rearing (R7). Transmission electron microscopy analysis showed swollen mitochondria with disintegration of cristae and typical characteristics of endoplasmic reticulum stress in crop tissues at R1 compared with those at I4. During the peak of pigeon milk formation, the concentrations of reactive oxygen species, and oxidative damage markers (advanced oxidation protein products, 8-hydroxy-2 deoxyguanosine, and malondialdehyde) and the enzyme activities of total superoxide dismutase and glutathione peroxidase were all elevated significantly (P < 0.05). The protein concentration of B-cell lymphoma-2 associated X in crop tissues was significantly higher at R1, while the level of B-cell lymphoma-2 protein in males was the highest at I4 (P < 0.05). The ratio of B-cell lymphoma-2 associated X protein (Bax)/B-cell lymphoma-2 (Bcl-2) in both male and female crops peaked at R1 (P < 0.05). Gene expression of the key enzymes involved in mitochondrial and peroxisomal fatty acid β-oxidation was investigated in crops. In males, the gene expression of carnitine palmitoyltransferase 1a peaked at R15, and that of carnitine palmitoyltransferase 2 increased significantly from R1 to R15 (P < 0.05). The mRNA abundance of long chain 3-hydroxyacyl-CoA dehydrogenase increased to the maximum value at R1 and I17 in males and females, respectively. From I17 to R7, the mRNA levels of acyl-CoA oxidase 1 and acyl-CoA oxidase 2 were decreased in pigeon crops (P < 0.05). Conclusively, lipid droplet accumulation was found in male and female pigeon crops from the end of incubation to the early stage of chick rearing. Although antioxidant defence and mitochondrial fatty acid β-oxidation were both mobilized, oxidative stress in crop tissues still occurred during the peak of milk formation. Elsevier 2022-10-26 /pmc/articles/PMC9706646/ /pubmed/36436376 http://dx.doi.org/10.1016/j.psj.2022.102289 Text en © 2022 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle PHYSIOLOGY AND REPRODUCTION
Xie, P.
Zhu, J.G.
Wang, L.X.
Liu, Y.
Diao, E.J.
Gong, D.Q.
Liu, T.W.
Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title_full Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title_fullStr Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title_full_unstemmed Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title_short Lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
title_sort lipid accumulation and oxidative stress in the crop tissues of male and female pigeons during incubation and chick-rearing periods
topic PHYSIOLOGY AND REPRODUCTION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706646/
https://www.ncbi.nlm.nih.gov/pubmed/36436376
http://dx.doi.org/10.1016/j.psj.2022.102289
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