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Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis

BACKGROUND: Postoperative insulin resistance (PIR) represents an important characteristic of metabolic response following surgical injury. Clinical outcomes are negatively correlated to postoperative insulin resistance and hyperglycemia, indicating a novel treatment for reducing postoperative insuli...

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Autores principales: Zhang, Jin, Chi, Rui, Zhang, Yunpeng, Xie, Yi, Liu, Yunxia, Bao, Qun, Lv, Hengyu, Han, Bo, Sun, Haipeng, Sun, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706859/
https://www.ncbi.nlm.nih.gov/pubmed/36447227
http://dx.doi.org/10.1186/s12986-022-00710-3
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author Zhang, Jin
Chi, Rui
Zhang, Yunpeng
Xie, Yi
Liu, Yunxia
Bao, Qun
Lv, Hengyu
Han, Bo
Sun, Haipeng
Sun, Peng
author_facet Zhang, Jin
Chi, Rui
Zhang, Yunpeng
Xie, Yi
Liu, Yunxia
Bao, Qun
Lv, Hengyu
Han, Bo
Sun, Haipeng
Sun, Peng
author_sort Zhang, Jin
collection PubMed
description BACKGROUND: Postoperative insulin resistance (PIR) represents an important characteristic of metabolic response following surgical injury. Clinical outcomes are negatively correlated to postoperative insulin resistance and hyperglycemia, indicating a novel treatment for reducing postoperative insulin resistance is urgently needed. The current work aimed to assess the protective effects of branched-chain amino acids (BCAA) on glucose metabolism disorders induced surgically in a rat model, and to explore the underpinning mechanism. METHODS AND RESULTS: Rats were randomly assigned to 2 groups, including the control and BCAA groups. Rats were given a compulsory oral 3 mL load by gavage two hours before surgery. The results showed that BCAA remarkably reduced glycemia by suppressing liver gluconeogenesis via reduction of cAMP-response element-binding protein-regulated transcription coactivator 2 (CRTC2) and glucose-6-phosphatase (G6PC) gene and protein expression levels (all Ps < 0.05). CONCLUSIONS: This study revealed that BCAA lower blood glucose levels by reducing liver gluconeogenesis without significant elevation of plasma insulin levels. We anticipate that preoperative BCAA supplementation may be a means for preventing postoperative insulin resistance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00710-3.
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spelling pubmed-97068592022-11-30 Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis Zhang, Jin Chi, Rui Zhang, Yunpeng Xie, Yi Liu, Yunxia Bao, Qun Lv, Hengyu Han, Bo Sun, Haipeng Sun, Peng Nutr Metab (Lond) Research BACKGROUND: Postoperative insulin resistance (PIR) represents an important characteristic of metabolic response following surgical injury. Clinical outcomes are negatively correlated to postoperative insulin resistance and hyperglycemia, indicating a novel treatment for reducing postoperative insulin resistance is urgently needed. The current work aimed to assess the protective effects of branched-chain amino acids (BCAA) on glucose metabolism disorders induced surgically in a rat model, and to explore the underpinning mechanism. METHODS AND RESULTS: Rats were randomly assigned to 2 groups, including the control and BCAA groups. Rats were given a compulsory oral 3 mL load by gavage two hours before surgery. The results showed that BCAA remarkably reduced glycemia by suppressing liver gluconeogenesis via reduction of cAMP-response element-binding protein-regulated transcription coactivator 2 (CRTC2) and glucose-6-phosphatase (G6PC) gene and protein expression levels (all Ps < 0.05). CONCLUSIONS: This study revealed that BCAA lower blood glucose levels by reducing liver gluconeogenesis without significant elevation of plasma insulin levels. We anticipate that preoperative BCAA supplementation may be a means for preventing postoperative insulin resistance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00710-3. BioMed Central 2022-11-29 /pmc/articles/PMC9706859/ /pubmed/36447227 http://dx.doi.org/10.1186/s12986-022-00710-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Jin
Chi, Rui
Zhang, Yunpeng
Xie, Yi
Liu, Yunxia
Bao, Qun
Lv, Hengyu
Han, Bo
Sun, Haipeng
Sun, Peng
Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title_full Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title_fullStr Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title_full_unstemmed Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title_short Preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
title_sort preoperative administration of branched-chain amino acids reduces postoperative insulin resistance in rats by reducing liver gluconeogenesis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706859/
https://www.ncbi.nlm.nih.gov/pubmed/36447227
http://dx.doi.org/10.1186/s12986-022-00710-3
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