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The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children

OBJECTIVE: The present study aims to explore the correlation of the transforming growth factor β (TGF-β), drosophila mothers against decapentaplegic protein gene (SMAD) 2/3/4, and leukemia inhibitory factors (LIF) with the cyst formation of hepatic Echinococcus granulosus in young children. METHODS:...

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Autores principales: Qin, Shuang-li, Guo, Yun, Li, Shui-Xue, Zhou, Ling, Maimaiti, Azguli, Akemu, Yusufu, He, Jun, Yao, Hai-Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706881/
https://www.ncbi.nlm.nih.gov/pubmed/36443650
http://dx.doi.org/10.1186/s12860-022-00452-3
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author Qin, Shuang-li
Guo, Yun
Li, Shui-Xue
Zhou, Ling
Maimaiti, Azguli
Akemu, Yusufu
He, Jun
Yao, Hai-Xia
author_facet Qin, Shuang-li
Guo, Yun
Li, Shui-Xue
Zhou, Ling
Maimaiti, Azguli
Akemu, Yusufu
He, Jun
Yao, Hai-Xia
author_sort Qin, Shuang-li
collection PubMed
description OBJECTIVE: The present study aims to explore the correlation of the transforming growth factor β (TGF-β), drosophila mothers against decapentaplegic protein gene (SMAD) 2/3/4, and leukemia inhibitory factors (LIF) with the cyst formation of hepatic Echinococcus granulosus in young children. METHODS: A total of 40 patients who met the diagnostic criteria for children's hydatid disease in people's Hospital of Xinjiang Uygur Autonomous Region between January 2020 and June 2021 were enrolled a s the study subjects. The cystic fluid of these children was collected as the case group and the corresponding infected viscera or pericystic tissue as the control group, with 40 cases in each group. In vitro cultured protoscolice of hydatid cyst, four groups including control group, LIF siRNA group, LIF factor group and SMAD4 siRNA group were divided by inhibiting TGF-β/SMADs signal pathway. Each assay was performed in triplicate. The expression of TGF-β, SMAD2/3/4 and LIF were detected. RESULTS: The results of the clinical trial showed that the contents of SMAD2 and SMAD3 were increased in the case group compared with the control group; the differences were statistically significant (P < 0.05). The expression levels of TGF-β, Smad4, and LIF increased in the case group compared with the control group; however, the differences were not statistically significant. The results of further in vitro experiments, the expression levels of TGF-β, SMAD 2/3/4, and LIF after adding siRNA to interfere with Smad4 decreased in the case group compared with the control group; the differences were statistically significant (P < 0.05). Compared with the control group, the expression levels of TGF-β, SMAD2/3/4, and LIF increased after treatment with added LIF in the case group, and the expression levels of TGF-β, SMAD2/3/4, and LIF decreased after adding siRNA to interfere with LIF in the case group; the differences were all statistically significant (P < 0.05). CONCLUSION: SMAD2 and SMAD3 have a certain clinical relevance with hydatidosis in young children. The LIF expression level may be related to the cystic transformation of protoscoleces. It has been suggested that the TGF-β/Smads/LIF signaling pathway may be present in the process of protoscoleces cyst formation; this provides a research basis for the prevention and treatment of post-infection parasitism of E. multilocularis eggs in young children.
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spelling pubmed-97068812022-11-30 The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children Qin, Shuang-li Guo, Yun Li, Shui-Xue Zhou, Ling Maimaiti, Azguli Akemu, Yusufu He, Jun Yao, Hai-Xia BMC Mol Cell Biol Research OBJECTIVE: The present study aims to explore the correlation of the transforming growth factor β (TGF-β), drosophila mothers against decapentaplegic protein gene (SMAD) 2/3/4, and leukemia inhibitory factors (LIF) with the cyst formation of hepatic Echinococcus granulosus in young children. METHODS: A total of 40 patients who met the diagnostic criteria for children's hydatid disease in people's Hospital of Xinjiang Uygur Autonomous Region between January 2020 and June 2021 were enrolled a s the study subjects. The cystic fluid of these children was collected as the case group and the corresponding infected viscera or pericystic tissue as the control group, with 40 cases in each group. In vitro cultured protoscolice of hydatid cyst, four groups including control group, LIF siRNA group, LIF factor group and SMAD4 siRNA group were divided by inhibiting TGF-β/SMADs signal pathway. Each assay was performed in triplicate. The expression of TGF-β, SMAD2/3/4 and LIF were detected. RESULTS: The results of the clinical trial showed that the contents of SMAD2 and SMAD3 were increased in the case group compared with the control group; the differences were statistically significant (P < 0.05). The expression levels of TGF-β, Smad4, and LIF increased in the case group compared with the control group; however, the differences were not statistically significant. The results of further in vitro experiments, the expression levels of TGF-β, SMAD 2/3/4, and LIF after adding siRNA to interfere with Smad4 decreased in the case group compared with the control group; the differences were statistically significant (P < 0.05). Compared with the control group, the expression levels of TGF-β, SMAD2/3/4, and LIF increased after treatment with added LIF in the case group, and the expression levels of TGF-β, SMAD2/3/4, and LIF decreased after adding siRNA to interfere with LIF in the case group; the differences were all statistically significant (P < 0.05). CONCLUSION: SMAD2 and SMAD3 have a certain clinical relevance with hydatidosis in young children. The LIF expression level may be related to the cystic transformation of protoscoleces. It has been suggested that the TGF-β/Smads/LIF signaling pathway may be present in the process of protoscoleces cyst formation; this provides a research basis for the prevention and treatment of post-infection parasitism of E. multilocularis eggs in young children. BioMed Central 2022-11-28 /pmc/articles/PMC9706881/ /pubmed/36443650 http://dx.doi.org/10.1186/s12860-022-00452-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Qin, Shuang-li
Guo, Yun
Li, Shui-Xue
Zhou, Ling
Maimaiti, Azguli
Akemu, Yusufu
He, Jun
Yao, Hai-Xia
The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title_full The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title_fullStr The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title_full_unstemmed The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title_short The role of the TGF-β/LIF signaling pathway mediated by SMADs during the cyst formation of Echinococcus in young children
title_sort role of the tgf-β/lif signaling pathway mediated by smads during the cyst formation of echinococcus in young children
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9706881/
https://www.ncbi.nlm.nih.gov/pubmed/36443650
http://dx.doi.org/10.1186/s12860-022-00452-3
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