Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro
BACKGROUND: Talaromyces marneffei (T. marneffei) is a thermally dimorphic fungus endemic to Southeast Asia that causes human systemic infection. Our earlier immunohistochemical studies revealed that the organisms were markedly labeled with the CD86 antigen in cutaneous lesions brought on by infectio...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Dove
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9707389/ https://www.ncbi.nlm.nih.gov/pubmed/36458198 http://dx.doi.org/10.2147/IDR.S389612 |
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author | Fang, Jinling Chen, Rifeng Liu, Donghua |
author_facet | Fang, Jinling Chen, Rifeng Liu, Donghua |
author_sort | Fang, Jinling |
collection | PubMed |
description | BACKGROUND: Talaromyces marneffei (T. marneffei) is a thermally dimorphic fungus endemic to Southeast Asia that causes human systemic infection. Our earlier immunohistochemical studies revealed that the organisms were markedly labeled with the CD86 antigen in cutaneous lesions brought on by infection. However, the relationship between T. marneffei and the CD86 co-stimulatory molecule is still unknown. OBJECTIVE: To explore the association between CD86 Protein and Talaromyces marneffei organisms in vitro and discuss the potential mechanisms. METHODS: We created the CD86-EGFP fusion protein in THP-1 macrophages and co-cultured T. marneffei conidia with it. We used confocal fluorescence microscopy to view in vitro dynamics. The link between CD86 Protein and Talaromyces marneffei organisms in vitro was discovered using immunoelectron microscopy, indirect immunofluorescence test, and immunohistochemistry assay. RESULTS: T. marneffei cells received soluble CD86-EGFP from THP-1 macrophages detected by confocal fluorescent microscopy. Both the indirect immunofluorescence assay and the immunohistochemical assay showed that T. marneffei conidia were stained by the CD86 marker. Immunoelectron microscopy showed that characteristic colloidal gold particles were observed in T. marneffei organisms when co-cultured with THP-1 macrophages. CONCLUSION: T. marneffei organisms have the ability to capture CD86 proteins from macrophages in vitro. |
format | Online Article Text |
id | pubmed-9707389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-97073892022-11-30 Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro Fang, Jinling Chen, Rifeng Liu, Donghua Infect Drug Resist Original Research BACKGROUND: Talaromyces marneffei (T. marneffei) is a thermally dimorphic fungus endemic to Southeast Asia that causes human systemic infection. Our earlier immunohistochemical studies revealed that the organisms were markedly labeled with the CD86 antigen in cutaneous lesions brought on by infection. However, the relationship between T. marneffei and the CD86 co-stimulatory molecule is still unknown. OBJECTIVE: To explore the association between CD86 Protein and Talaromyces marneffei organisms in vitro and discuss the potential mechanisms. METHODS: We created the CD86-EGFP fusion protein in THP-1 macrophages and co-cultured T. marneffei conidia with it. We used confocal fluorescence microscopy to view in vitro dynamics. The link between CD86 Protein and Talaromyces marneffei organisms in vitro was discovered using immunoelectron microscopy, indirect immunofluorescence test, and immunohistochemistry assay. RESULTS: T. marneffei cells received soluble CD86-EGFP from THP-1 macrophages detected by confocal fluorescent microscopy. Both the indirect immunofluorescence assay and the immunohistochemical assay showed that T. marneffei conidia were stained by the CD86 marker. Immunoelectron microscopy showed that characteristic colloidal gold particles were observed in T. marneffei organisms when co-cultured with THP-1 macrophages. CONCLUSION: T. marneffei organisms have the ability to capture CD86 proteins from macrophages in vitro. Dove 2022-11-25 /pmc/articles/PMC9707389/ /pubmed/36458198 http://dx.doi.org/10.2147/IDR.S389612 Text en © 2022 Fang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Fang, Jinling Chen, Rifeng Liu, Donghua Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title | Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title_full | Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title_fullStr | Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title_full_unstemmed | Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title_short | Talaromyces marneffei Can Capture CD86 Proteins of Macrophages in vitro |
title_sort | talaromyces marneffei can capture cd86 proteins of macrophages in vitro |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9707389/ https://www.ncbi.nlm.nih.gov/pubmed/36458198 http://dx.doi.org/10.2147/IDR.S389612 |
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