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Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities
Citrus reticulata “Chachi” (CRC) leaves contain abundant flavonoids, indicating that they possess good nutritional/pharmacological research and development potential. This study aims to explore chemical antioxidant quality markers based on the spectrum-effect relationship and quality control strateg...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9708335/ https://www.ncbi.nlm.nih.gov/pubmed/36458111 http://dx.doi.org/10.1155/2022/5834525 |
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author | Nie, Qin-Xin Zhang, Qiu-Xia Zhu, Yin-Ji Wu, Pei-Jun He, Ya-Li Wang, Jin-Yu |
author_facet | Nie, Qin-Xin Zhang, Qiu-Xia Zhu, Yin-Ji Wu, Pei-Jun He, Ya-Li Wang, Jin-Yu |
author_sort | Nie, Qin-Xin |
collection | PubMed |
description | Citrus reticulata “Chachi” (CRC) leaves contain abundant flavonoids, indicating that they possess good nutritional/pharmacological research and development potential. This study aims to explore chemical antioxidant quality markers based on the spectrum-effect relationship and quality control strategy of CRC leaves. The ultrahigh performance liquid chromatography (UPLC) system was used to establish chromatographic fingerprints of Citrus reticulata “Chachi” leaves. Simultaneously, they were evaluated by using similarity analysis (SA), hierarchical cluster analysis (HCA), and principal component analysis (PCA). Afterwards, the DPPH assay was adopted to study the antioxidant effects. The spectrum-effect relationship between UPLC fingerprints and DPPH radical-scavenging activities was studied with grey relational analysis (GRA). Analysis results indicated that there were twenty-one common peaks of fourteen batches of CRC leaves which were from different regions of Guangdong province, and their similarities ranged from 0.648 to 0.997. HCA results showed that fourteen batches of samples of CRC leaves could be divided into six classes at Euclidean distance of 5. The results from GRA showed that tangeretin and hesperidin were the main flavonoids responsible for the antioxidant activity in CRC leaves. In conclusion, this research established a chromatographic analysis method suitable for CRC leaves and demonstrated that chromatographic fingerprints analysis combined with the antioxidant activity could be used to evaluate the material basis of CRC leaves and may provide a reference to establish a quality standard. |
format | Online Article Text |
id | pubmed-9708335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-97083352022-11-30 Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities Nie, Qin-Xin Zhang, Qiu-Xia Zhu, Yin-Ji Wu, Pei-Jun He, Ya-Li Wang, Jin-Yu Int J Anal Chem Research Article Citrus reticulata “Chachi” (CRC) leaves contain abundant flavonoids, indicating that they possess good nutritional/pharmacological research and development potential. This study aims to explore chemical antioxidant quality markers based on the spectrum-effect relationship and quality control strategy of CRC leaves. The ultrahigh performance liquid chromatography (UPLC) system was used to establish chromatographic fingerprints of Citrus reticulata “Chachi” leaves. Simultaneously, they were evaluated by using similarity analysis (SA), hierarchical cluster analysis (HCA), and principal component analysis (PCA). Afterwards, the DPPH assay was adopted to study the antioxidant effects. The spectrum-effect relationship between UPLC fingerprints and DPPH radical-scavenging activities was studied with grey relational analysis (GRA). Analysis results indicated that there were twenty-one common peaks of fourteen batches of CRC leaves which were from different regions of Guangdong province, and their similarities ranged from 0.648 to 0.997. HCA results showed that fourteen batches of samples of CRC leaves could be divided into six classes at Euclidean distance of 5. The results from GRA showed that tangeretin and hesperidin were the main flavonoids responsible for the antioxidant activity in CRC leaves. In conclusion, this research established a chromatographic analysis method suitable for CRC leaves and demonstrated that chromatographic fingerprints analysis combined with the antioxidant activity could be used to evaluate the material basis of CRC leaves and may provide a reference to establish a quality standard. Hindawi 2022-11-22 /pmc/articles/PMC9708335/ /pubmed/36458111 http://dx.doi.org/10.1155/2022/5834525 Text en Copyright © 2022 Qin-Xin Nie et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Nie, Qin-Xin Zhang, Qiu-Xia Zhu, Yin-Ji Wu, Pei-Jun He, Ya-Li Wang, Jin-Yu Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title | Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title_full | Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title_fullStr | Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title_full_unstemmed | Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title_short | Relationship between the UPLC Fingerprints of Citrus reticulata “Chachi” Leaves and Their Antioxidant Activities |
title_sort | relationship between the uplc fingerprints of citrus reticulata “chachi” leaves and their antioxidant activities |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9708335/ https://www.ncbi.nlm.nih.gov/pubmed/36458111 http://dx.doi.org/10.1155/2022/5834525 |
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