Cargando…
Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro
BACKGROUND: The SARS-CoV-2/COVID-19 pandemic has inflicted medical and socioeconomic havoc, and despite the current availability of vaccines and broad implementation of vaccination programs, more easily accessible and cost-effective acute treatment options preventing morbidity and mortality are urge...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9708519/ https://www.ncbi.nlm.nih.gov/pubmed/36447206 http://dx.doi.org/10.1186/s12915-022-01468-z |
_version_ | 1784840953312313344 |
---|---|
author | Le-Trilling, Vu Thuy Khanh Mennerich, Denise Schuler, Corinna Sakson, Roman Lill, Julia K. Kasarla, Siva Swapna Kopczynski, Dominik Loroch, Stefan Flores-Martinez, Yulia Katschinski, Benjamin Wohlgemuth, Kerstin Gunzer, Matthias Meyer, Folker Phapale, Prasad Dittmer, Ulf Sickmann, Albert Trilling, Mirko |
author_facet | Le-Trilling, Vu Thuy Khanh Mennerich, Denise Schuler, Corinna Sakson, Roman Lill, Julia K. Kasarla, Siva Swapna Kopczynski, Dominik Loroch, Stefan Flores-Martinez, Yulia Katschinski, Benjamin Wohlgemuth, Kerstin Gunzer, Matthias Meyer, Folker Phapale, Prasad Dittmer, Ulf Sickmann, Albert Trilling, Mirko |
author_sort | Le-Trilling, Vu Thuy Khanh |
collection | PubMed |
description | BACKGROUND: The SARS-CoV-2/COVID-19 pandemic has inflicted medical and socioeconomic havoc, and despite the current availability of vaccines and broad implementation of vaccination programs, more easily accessible and cost-effective acute treatment options preventing morbidity and mortality are urgently needed. Herbal teas have historically and recurrently been applied as self-medication for prophylaxis, therapy, and symptom alleviation in diverse diseases, including those caused by respiratory viruses, and have provided sources of natural products as basis for the development of therapeutic agents. To identify affordable, ubiquitously available, and effective treatments, we tested herbs consumed worldwide as herbal teas regarding their antiviral activity against SARS-CoV-2. RESULTS: Aqueous infusions prepared by boiling leaves of the Lamiaceae perilla and sage elicit potent and sustained antiviral activity against SARS-CoV-2 when applied after infection as well as prior to infection of cells. The herbal infusions exerted in vitro antiviral effects comparable to interferon-β and remdesivir but outperformed convalescent sera and interferon-α2 upon short-term treatment early after infection. Based on protein fractionation analyses, we identified caffeic acid, perilla aldehyde, and perillyl alcohol as antiviral compounds. Global mass spectrometry (MS) analyses performed comparatively in two different cell culture infection models revealed changes of the proteome upon treatment with herbal infusions and provided insights into the mode of action. As inferred by the MS data, induction of heme oxygenase 1 (HMOX-1) was confirmed as effector mechanism by the antiviral activity of the HMOX-1-inducing compounds sulforaphane and fraxetin. CONCLUSIONS: In conclusion, herbal teas based on perilla and sage exhibit antiviral activity against SARS-CoV-2 including variants of concern such as Alpha, Beta, Delta, and Omicron, and we identified HMOX-1 as potential therapeutic target. Given that perilla and sage have been suggested as treatment options for various diseases, our dataset may constitute a valuable resource also for future research beyond virology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01468-z. |
format | Online Article Text |
id | pubmed-9708519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97085192022-11-30 Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro Le-Trilling, Vu Thuy Khanh Mennerich, Denise Schuler, Corinna Sakson, Roman Lill, Julia K. Kasarla, Siva Swapna Kopczynski, Dominik Loroch, Stefan Flores-Martinez, Yulia Katschinski, Benjamin Wohlgemuth, Kerstin Gunzer, Matthias Meyer, Folker Phapale, Prasad Dittmer, Ulf Sickmann, Albert Trilling, Mirko BMC Biol Research Article BACKGROUND: The SARS-CoV-2/COVID-19 pandemic has inflicted medical and socioeconomic havoc, and despite the current availability of vaccines and broad implementation of vaccination programs, more easily accessible and cost-effective acute treatment options preventing morbidity and mortality are urgently needed. Herbal teas have historically and recurrently been applied as self-medication for prophylaxis, therapy, and symptom alleviation in diverse diseases, including those caused by respiratory viruses, and have provided sources of natural products as basis for the development of therapeutic agents. To identify affordable, ubiquitously available, and effective treatments, we tested herbs consumed worldwide as herbal teas regarding their antiviral activity against SARS-CoV-2. RESULTS: Aqueous infusions prepared by boiling leaves of the Lamiaceae perilla and sage elicit potent and sustained antiviral activity against SARS-CoV-2 when applied after infection as well as prior to infection of cells. The herbal infusions exerted in vitro antiviral effects comparable to interferon-β and remdesivir but outperformed convalescent sera and interferon-α2 upon short-term treatment early after infection. Based on protein fractionation analyses, we identified caffeic acid, perilla aldehyde, and perillyl alcohol as antiviral compounds. Global mass spectrometry (MS) analyses performed comparatively in two different cell culture infection models revealed changes of the proteome upon treatment with herbal infusions and provided insights into the mode of action. As inferred by the MS data, induction of heme oxygenase 1 (HMOX-1) was confirmed as effector mechanism by the antiviral activity of the HMOX-1-inducing compounds sulforaphane and fraxetin. CONCLUSIONS: In conclusion, herbal teas based on perilla and sage exhibit antiviral activity against SARS-CoV-2 including variants of concern such as Alpha, Beta, Delta, and Omicron, and we identified HMOX-1 as potential therapeutic target. Given that perilla and sage have been suggested as treatment options for various diseases, our dataset may constitute a valuable resource also for future research beyond virology. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01468-z. BioMed Central 2022-11-30 /pmc/articles/PMC9708519/ /pubmed/36447206 http://dx.doi.org/10.1186/s12915-022-01468-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Le-Trilling, Vu Thuy Khanh Mennerich, Denise Schuler, Corinna Sakson, Roman Lill, Julia K. Kasarla, Siva Swapna Kopczynski, Dominik Loroch, Stefan Flores-Martinez, Yulia Katschinski, Benjamin Wohlgemuth, Kerstin Gunzer, Matthias Meyer, Folker Phapale, Prasad Dittmer, Ulf Sickmann, Albert Trilling, Mirko Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title | Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title_full | Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title_fullStr | Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title_full_unstemmed | Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title_short | Identification of herbal teas and their compounds eliciting antiviral activity against SARS-CoV-2 in vitro |
title_sort | identification of herbal teas and their compounds eliciting antiviral activity against sars-cov-2 in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9708519/ https://www.ncbi.nlm.nih.gov/pubmed/36447206 http://dx.doi.org/10.1186/s12915-022-01468-z |
work_keys_str_mv | AT letrillingvuthuykhanh identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT mennerichdenise identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT schulercorinna identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT saksonroman identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT lilljuliak identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT kasarlasivaswapna identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT kopczynskidominik identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT lorochstefan identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT floresmartinezyulia identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT katschinskibenjamin identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT wohlgemuthkerstin identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT gunzermatthias identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT meyerfolker identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT phapaleprasad identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT dittmerulf identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT sickmannalbert identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro AT trillingmirko identificationofherbalteasandtheircompoundselicitingantiviralactivityagainstsarscov2invitro |