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Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges

Little is known about the interplay between the ruminant microbiome and the host during challenging events. This long-term study investigated the ruminal and duodenal microbiome and metabolites during calving as an individual challenge and a lipopolysaccharide-induced systemic inflammation as a stan...

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Autores principales: Tröscher-Mußotter, Johanna, Deusch, Simon, Borda-Molina, Daniel, Frahm, Jana, Dänicke, Sven, Camarinha-Silva, Amélia, Huber, Korinna, Seifert, Jana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709127/
https://www.ncbi.nlm.nih.gov/pubmed/36466656
http://dx.doi.org/10.3389/fmicb.2022.1000750
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author Tröscher-Mußotter, Johanna
Deusch, Simon
Borda-Molina, Daniel
Frahm, Jana
Dänicke, Sven
Camarinha-Silva, Amélia
Huber, Korinna
Seifert, Jana
author_facet Tröscher-Mußotter, Johanna
Deusch, Simon
Borda-Molina, Daniel
Frahm, Jana
Dänicke, Sven
Camarinha-Silva, Amélia
Huber, Korinna
Seifert, Jana
author_sort Tröscher-Mußotter, Johanna
collection PubMed
description Little is known about the interplay between the ruminant microbiome and the host during challenging events. This long-term study investigated the ruminal and duodenal microbiome and metabolites during calving as an individual challenge and a lipopolysaccharide-induced systemic inflammation as a standardized challenge. Strong inter- and intra-individual microbiome changes were noted during the entire trial period of 168 days and between the 12 sampling time points. Bifidobacterium increased significantly at 3 days after calving. Both challenges increased the intestinal abundance of fiber-associated taxa, e.g., Butyrivibrio and unclassified Ruminococcaceae. NMR analyses of rumen and duodenum samples identified up to 60 metabolites out of which fatty and amino acids, amines, and urea varied in concentrations triggered by the two challenges. Correlation analyses between these parameters indicated a close connection and dependency of the microbiome with its host. It turns out that the combination of phylogenetic with metabolite information supports the understanding of the true scenario in the forestomach system. The individual stages of the production cycle in dairy cows reveal specific criteria for the interaction pattern between microbial functions and host responses.
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spelling pubmed-97091272022-12-01 Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges Tröscher-Mußotter, Johanna Deusch, Simon Borda-Molina, Daniel Frahm, Jana Dänicke, Sven Camarinha-Silva, Amélia Huber, Korinna Seifert, Jana Front Microbiol Microbiology Little is known about the interplay between the ruminant microbiome and the host during challenging events. This long-term study investigated the ruminal and duodenal microbiome and metabolites during calving as an individual challenge and a lipopolysaccharide-induced systemic inflammation as a standardized challenge. Strong inter- and intra-individual microbiome changes were noted during the entire trial period of 168 days and between the 12 sampling time points. Bifidobacterium increased significantly at 3 days after calving. Both challenges increased the intestinal abundance of fiber-associated taxa, e.g., Butyrivibrio and unclassified Ruminococcaceae. NMR analyses of rumen and duodenum samples identified up to 60 metabolites out of which fatty and amino acids, amines, and urea varied in concentrations triggered by the two challenges. Correlation analyses between these parameters indicated a close connection and dependency of the microbiome with its host. It turns out that the combination of phylogenetic with metabolite information supports the understanding of the true scenario in the forestomach system. The individual stages of the production cycle in dairy cows reveal specific criteria for the interaction pattern between microbial functions and host responses. Frontiers Media S.A. 2022-11-16 /pmc/articles/PMC9709127/ /pubmed/36466656 http://dx.doi.org/10.3389/fmicb.2022.1000750 Text en Copyright © 2022 Tröscher-Mußotter, Deusch, Borda-Molina, Frahm, Dänicke, Camarinha-Silva, Huber and Seifert. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tröscher-Mußotter, Johanna
Deusch, Simon
Borda-Molina, Daniel
Frahm, Jana
Dänicke, Sven
Camarinha-Silva, Amélia
Huber, Korinna
Seifert, Jana
Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title_full Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title_fullStr Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title_full_unstemmed Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title_short Cow’s microbiome from antepartum to postpartum: A long-term study covering two physiological challenges
title_sort cow’s microbiome from antepartum to postpartum: a long-term study covering two physiological challenges
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709127/
https://www.ncbi.nlm.nih.gov/pubmed/36466656
http://dx.doi.org/10.3389/fmicb.2022.1000750
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