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Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype
Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the M...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709149/ https://www.ncbi.nlm.nih.gov/pubmed/36466658 http://dx.doi.org/10.3389/fmicb.2022.1011578 |
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author | Jesus, Hendor N. R. Rocha, Danilo J. P. G. Ramos, Rommel T. J. Silva, Artur Brenig, Bertram Góes-Neto, Aristóteles Costa, Mateus M. Soares, Siomar C. Azevedo, Vasco Aguiar, Eric R. G. R. Martínez-Martínez, Luiz Ocampo, Alain Alibi, Sana Dorta, Alexis Pacheco, Luis G. C. Navas, Jesus |
author_facet | Jesus, Hendor N. R. Rocha, Danilo J. P. G. Ramos, Rommel T. J. Silva, Artur Brenig, Bertram Góes-Neto, Aristóteles Costa, Mateus M. Soares, Siomar C. Azevedo, Vasco Aguiar, Eric R. G. R. Martínez-Martínez, Luiz Ocampo, Alain Alibi, Sana Dorta, Alexis Pacheco, Luis G. C. Navas, Jesus |
author_sort | Jesus, Hendor N. R. |
collection | PubMed |
description | Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the MDR invasive phenotype in clinical isolates. In this study, we performed a comprehensive pan-genomic analysis of C. amycolatum, including 26 isolates from different countries. We obtained the novel genome sequences of 8 of them, which are multidrug resistant clinical isolates from Spain and Tunisia. They were analyzed together with other 18 complete or draft C. amycolatum genomes retrieved from GenBank. The species C. amycolatum presented an open pan-genome (α = 0.854905), with 3,280 gene families, being 1,690 (51.52%) in the core genome, 1,121 related to accessory genes (34.17%), and 469 related to unique genes (14.29%). Although some classic corynebacterial virulence factors are absent in the species C. amycolatum, we did identify genes associated with immune evasion, toxin, and antiphagocytosis among the predicted putative virulence factors. Additionally, we found genomic evidence for extensive acquisition of antimicrobial resistance genes through genomic islands. |
format | Online Article Text |
id | pubmed-9709149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97091492022-12-01 Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype Jesus, Hendor N. R. Rocha, Danilo J. P. G. Ramos, Rommel T. J. Silva, Artur Brenig, Bertram Góes-Neto, Aristóteles Costa, Mateus M. Soares, Siomar C. Azevedo, Vasco Aguiar, Eric R. G. R. Martínez-Martínez, Luiz Ocampo, Alain Alibi, Sana Dorta, Alexis Pacheco, Luis G. C. Navas, Jesus Front Microbiol Microbiology Corynebacterium amycolatum is a nonlipophilic coryneform which is increasingly being recognized as a relevant human and animal pathogen showing multidrug resistance to commonly used antibiotics. However, little is known about the molecular mechanisms involved in transition from colonization to the MDR invasive phenotype in clinical isolates. In this study, we performed a comprehensive pan-genomic analysis of C. amycolatum, including 26 isolates from different countries. We obtained the novel genome sequences of 8 of them, which are multidrug resistant clinical isolates from Spain and Tunisia. They were analyzed together with other 18 complete or draft C. amycolatum genomes retrieved from GenBank. The species C. amycolatum presented an open pan-genome (α = 0.854905), with 3,280 gene families, being 1,690 (51.52%) in the core genome, 1,121 related to accessory genes (34.17%), and 469 related to unique genes (14.29%). Although some classic corynebacterial virulence factors are absent in the species C. amycolatum, we did identify genes associated with immune evasion, toxin, and antiphagocytosis among the predicted putative virulence factors. Additionally, we found genomic evidence for extensive acquisition of antimicrobial resistance genes through genomic islands. Frontiers Media S.A. 2022-11-16 /pmc/articles/PMC9709149/ /pubmed/36466658 http://dx.doi.org/10.3389/fmicb.2022.1011578 Text en Copyright © 2022 Jesus, Rocha, Ramos, Silva, Brenig, Góes-Neto, Costa, Soares, Azevedo, Aguiar, Martínez-Martínez, Ocampo, Alibi, Dorta, Pacheco and Navas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Jesus, Hendor N. R. Rocha, Danilo J. P. G. Ramos, Rommel T. J. Silva, Artur Brenig, Bertram Góes-Neto, Aristóteles Costa, Mateus M. Soares, Siomar C. Azevedo, Vasco Aguiar, Eric R. G. R. Martínez-Martínez, Luiz Ocampo, Alain Alibi, Sana Dorta, Alexis Pacheco, Luis G. C. Navas, Jesus Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title | Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title_full | Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title_fullStr | Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title_full_unstemmed | Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title_short | Pan-genomic analysis of Corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
title_sort | pan-genomic analysis of corynebacterium amycolatum gives insights into molecular mechanisms underpinning the transition to a pathogenic phenotype |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709149/ https://www.ncbi.nlm.nih.gov/pubmed/36466658 http://dx.doi.org/10.3389/fmicb.2022.1011578 |
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