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Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing

AIMS: Epilepsy is a neurological disease occurring worldwide. Alterations in the gut microbial composition may be involved in the development of Epilepsy. The study aimed to investigate the effects of cannabidiol (CBD) on gut microbiota and the metabolic profile of epileptic rats. MATERIALS AND METH...

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Autores principales: Gong, Xiaoxiang, Liu, Lingjuan, Li, Xingfang, Xiong, Jie, Xu, Jie, Mao, Dingan, Liu, Liqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709218/
https://www.ncbi.nlm.nih.gov/pubmed/36466385
http://dx.doi.org/10.3389/fnut.2022.1028459
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author Gong, Xiaoxiang
Liu, Lingjuan
Li, Xingfang
Xiong, Jie
Xu, Jie
Mao, Dingan
Liu, Liqun
author_facet Gong, Xiaoxiang
Liu, Lingjuan
Li, Xingfang
Xiong, Jie
Xu, Jie
Mao, Dingan
Liu, Liqun
author_sort Gong, Xiaoxiang
collection PubMed
description AIMS: Epilepsy is a neurological disease occurring worldwide. Alterations in the gut microbial composition may be involved in the development of Epilepsy. The study aimed to investigate the effects of cannabidiol (CBD) on gut microbiota and the metabolic profile of epileptic rats. MATERIALS AND METHODS AND RESULTS: A temporal lobe epilepsy rat model was established using Li-pilocarpine. CBD increased the incubation period and reduced the epileptic state in rats. Compared to epileptic rats, the M1/M2 ratio of microglia in the CBD group was significantly decreased. The expression of IL-1β, IL-6, and TNF-α in the CBD group decreased, while IL-10, IL-4, and TGF-β1 increased. 16S rDNA sequencing revealed that the ANOSIM index differed significantly between the groups. At the genus level, Helicobacter, Prevotellaceae_UCG-001, and Ruminococcaceae_UCG-005 were significantly reduced in the model group. CBD intervention attenuated the intervention effects of Li-pilocarpine. Roseburia, Eubacterium_xylanophilum_group, and Ruminococcus_2 were strongly positively correlated with proinflammatory cytokine levels. CBD reversed dysregulated metabolites, including glycerophosphocholine and 4-ethylbenzoic acid. CONCLUSION: CBD could alleviate the dysbiosis of gut microbiota and metabolic disorders of epileptic rats. CBD attenuated Epilepsy in rats might be related to gut microbial abundance and metabolite levels. SIGNIFICANCE AND IMPACT OF STUDY: The study may provide a reliable scientific clue to explore the regulatory pathway of CBD in alleviating Epilepsy.
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spelling pubmed-97092182022-12-01 Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing Gong, Xiaoxiang Liu, Lingjuan Li, Xingfang Xiong, Jie Xu, Jie Mao, Dingan Liu, Liqun Front Nutr Nutrition AIMS: Epilepsy is a neurological disease occurring worldwide. Alterations in the gut microbial composition may be involved in the development of Epilepsy. The study aimed to investigate the effects of cannabidiol (CBD) on gut microbiota and the metabolic profile of epileptic rats. MATERIALS AND METHODS AND RESULTS: A temporal lobe epilepsy rat model was established using Li-pilocarpine. CBD increased the incubation period and reduced the epileptic state in rats. Compared to epileptic rats, the M1/M2 ratio of microglia in the CBD group was significantly decreased. The expression of IL-1β, IL-6, and TNF-α in the CBD group decreased, while IL-10, IL-4, and TGF-β1 increased. 16S rDNA sequencing revealed that the ANOSIM index differed significantly between the groups. At the genus level, Helicobacter, Prevotellaceae_UCG-001, and Ruminococcaceae_UCG-005 were significantly reduced in the model group. CBD intervention attenuated the intervention effects of Li-pilocarpine. Roseburia, Eubacterium_xylanophilum_group, and Ruminococcus_2 were strongly positively correlated with proinflammatory cytokine levels. CBD reversed dysregulated metabolites, including glycerophosphocholine and 4-ethylbenzoic acid. CONCLUSION: CBD could alleviate the dysbiosis of gut microbiota and metabolic disorders of epileptic rats. CBD attenuated Epilepsy in rats might be related to gut microbial abundance and metabolite levels. SIGNIFICANCE AND IMPACT OF STUDY: The study may provide a reliable scientific clue to explore the regulatory pathway of CBD in alleviating Epilepsy. Frontiers Media S.A. 2022-11-16 /pmc/articles/PMC9709218/ /pubmed/36466385 http://dx.doi.org/10.3389/fnut.2022.1028459 Text en Copyright © 2022 Gong, Liu, Li, Xiong, Xu, Mao and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Gong, Xiaoxiang
Liu, Lingjuan
Li, Xingfang
Xiong, Jie
Xu, Jie
Mao, Dingan
Liu, Liqun
Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title_full Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title_fullStr Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title_full_unstemmed Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title_short Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing
title_sort neuroprotection of cannabidiol in epileptic rats: gut microbiome and metabolome sequencing
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709218/
https://www.ncbi.nlm.nih.gov/pubmed/36466385
http://dx.doi.org/10.3389/fnut.2022.1028459
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