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Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations

Circulating cell-free DNA (cfDNA) analysis has the potential to revolutionise the care of patients with cancer and is already moving towards standard of care in some adult malignancies. Evidence for the utility of cfDNA analysis in paediatric cancer patients is also accumulating. In this review we d...

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Autores principales: Stankunaite, Reda, Marshall, Lynley V., Carceller, Fernando, Chesler, Louis, Hubank, Michael, George, Sally L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709284/
https://www.ncbi.nlm.nih.gov/pubmed/36467471
http://dx.doi.org/10.3389/fped.2022.957944
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author Stankunaite, Reda
Marshall, Lynley V.
Carceller, Fernando
Chesler, Louis
Hubank, Michael
George, Sally L.
author_facet Stankunaite, Reda
Marshall, Lynley V.
Carceller, Fernando
Chesler, Louis
Hubank, Michael
George, Sally L.
author_sort Stankunaite, Reda
collection PubMed
description Circulating cell-free DNA (cfDNA) analysis has the potential to revolutionise the care of patients with cancer and is already moving towards standard of care in some adult malignancies. Evidence for the utility of cfDNA analysis in paediatric cancer patients is also accumulating. In this review we discuss the limitations of blood-based assays in patients with brain tumours and describe the evidence supporting cerebrospinal fluid (CSF) cfDNA analysis. We make recommendations for CSF cfDNA processing to aid the standardisation and technical validation of future assays. We discuss the considerations for interpretation of cfDNA analysis and highlight promising future directions. Overall, cfDNA profiling shows great potential as an adjunct to the analysis of biopsy tissue in paediatric cancer patients, with the potential to provide a genetic molecular profile of the tumour when tissue biopsy is not feasible. However, to fully realise the potential of cfDNA analysis for children with brain tumours larger prospective studies incorporating serial CSF sampling are required.
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spelling pubmed-97092842022-12-01 Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations Stankunaite, Reda Marshall, Lynley V. Carceller, Fernando Chesler, Louis Hubank, Michael George, Sally L. Front Pediatr Pediatrics Circulating cell-free DNA (cfDNA) analysis has the potential to revolutionise the care of patients with cancer and is already moving towards standard of care in some adult malignancies. Evidence for the utility of cfDNA analysis in paediatric cancer patients is also accumulating. In this review we discuss the limitations of blood-based assays in patients with brain tumours and describe the evidence supporting cerebrospinal fluid (CSF) cfDNA analysis. We make recommendations for CSF cfDNA processing to aid the standardisation and technical validation of future assays. We discuss the considerations for interpretation of cfDNA analysis and highlight promising future directions. Overall, cfDNA profiling shows great potential as an adjunct to the analysis of biopsy tissue in paediatric cancer patients, with the potential to provide a genetic molecular profile of the tumour when tissue biopsy is not feasible. However, to fully realise the potential of cfDNA analysis for children with brain tumours larger prospective studies incorporating serial CSF sampling are required. Frontiers Media S.A. 2022-11-16 /pmc/articles/PMC9709284/ /pubmed/36467471 http://dx.doi.org/10.3389/fped.2022.957944 Text en © 2022 Stankunaite, Marshall, Carceller, Chesler, Hubank and George. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
Stankunaite, Reda
Marshall, Lynley V.
Carceller, Fernando
Chesler, Louis
Hubank, Michael
George, Sally L.
Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title_full Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title_fullStr Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title_full_unstemmed Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title_short Liquid biopsy for children with central nervous system tumours: Clinical integration and technical considerations
title_sort liquid biopsy for children with central nervous system tumours: clinical integration and technical considerations
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709284/
https://www.ncbi.nlm.nih.gov/pubmed/36467471
http://dx.doi.org/10.3389/fped.2022.957944
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