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Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis

Takayasu arteritis (TA) is a chronic granulomatous vasculitis involving in the main branches of aorta. Previous studies mainly used peripheral blood and some vascular tissues but seldom studies have sequenced vascular tissues. Here in this study, we aimed to explore the alterations of mRNA in TA by...

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Autores principales: Yuqing, Miao, Shang, Gao, Qing, Gao, Jiyang, Wang, Ruihao, Li, Zuoguan, Chen, Yongpeng, Diao, Zhiyuan, Wu, Yongjun, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709398/
https://www.ncbi.nlm.nih.gov/pubmed/36468014
http://dx.doi.org/10.3389/fgene.2022.1036233
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author Yuqing, Miao
Shang, Gao
Qing, Gao
Jiyang, Wang
Ruihao, Li
Zuoguan, Chen
Yongpeng, Diao
Zhiyuan, Wu
Yongjun, Li
author_facet Yuqing, Miao
Shang, Gao
Qing, Gao
Jiyang, Wang
Ruihao, Li
Zuoguan, Chen
Yongpeng, Diao
Zhiyuan, Wu
Yongjun, Li
author_sort Yuqing, Miao
collection PubMed
description Takayasu arteritis (TA) is a chronic granulomatous vasculitis involving in the main branches of aorta. Previous studies mainly used peripheral blood and some vascular tissues but seldom studies have sequenced vascular tissues. Here in this study, we aimed to explore the alterations of mRNA in TA by performing bulk RNA sequencing. A total of 14 abdominal aortic tissues including 8 from renal transplantation and 6 from patient with TA undergoing bypass surgeries. Bulk RNA sequencing were performed and after the quality control, a total of 1897 transcripts were observed to be significantly differently (p < 0.05 and Log(2)FC > 1) expressed between the TA and control group, among which 1,361 transcripts were in TA group and 536 in the Control group. Reactome Pathway Enrichment Comparison analysis revealed interleukin-10 signaling and signaling by interleukins were highly expressed in TA group. Besides, extracellular matrix organization was also observed in this group. WGCNA and PPI obtained 26 core genes which were highly correlated with the clinical phenotype. We then also perform deconvolution of the bulk RNA-seq data by using the scRNA-seq dataset and noticed the high proportion of smooth muscle cells in our dataset. Additionally, immunohistochemical staining confirmed our bioinformatic analysis that TA aortic tissues express high levels of IL-1R1 and IL-1R2. Briefly, this study revealed critical roles of interleukins in TA pathogenesis, and SMCs may also participate in the reconstruction in vessel wall at late stage of TA.
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spelling pubmed-97093982022-12-01 Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis Yuqing, Miao Shang, Gao Qing, Gao Jiyang, Wang Ruihao, Li Zuoguan, Chen Yongpeng, Diao Zhiyuan, Wu Yongjun, Li Front Genet Genetics Takayasu arteritis (TA) is a chronic granulomatous vasculitis involving in the main branches of aorta. Previous studies mainly used peripheral blood and some vascular tissues but seldom studies have sequenced vascular tissues. Here in this study, we aimed to explore the alterations of mRNA in TA by performing bulk RNA sequencing. A total of 14 abdominal aortic tissues including 8 from renal transplantation and 6 from patient with TA undergoing bypass surgeries. Bulk RNA sequencing were performed and after the quality control, a total of 1897 transcripts were observed to be significantly differently (p < 0.05 and Log(2)FC > 1) expressed between the TA and control group, among which 1,361 transcripts were in TA group and 536 in the Control group. Reactome Pathway Enrichment Comparison analysis revealed interleukin-10 signaling and signaling by interleukins were highly expressed in TA group. Besides, extracellular matrix organization was also observed in this group. WGCNA and PPI obtained 26 core genes which were highly correlated with the clinical phenotype. We then also perform deconvolution of the bulk RNA-seq data by using the scRNA-seq dataset and noticed the high proportion of smooth muscle cells in our dataset. Additionally, immunohistochemical staining confirmed our bioinformatic analysis that TA aortic tissues express high levels of IL-1R1 and IL-1R2. Briefly, this study revealed critical roles of interleukins in TA pathogenesis, and SMCs may also participate in the reconstruction in vessel wall at late stage of TA. Frontiers Media S.A. 2022-11-16 /pmc/articles/PMC9709398/ /pubmed/36468014 http://dx.doi.org/10.3389/fgene.2022.1036233 Text en Copyright © 2022 Yuqing, Shang, Qing, Jiyang, Ruihao, Zuoguan, Yongpeng, Zhiyuan and Yongjun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Yuqing, Miao
Shang, Gao
Qing, Gao
Jiyang, Wang
Ruihao, Li
Zuoguan, Chen
Yongpeng, Diao
Zhiyuan, Wu
Yongjun, Li
Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title_full Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title_fullStr Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title_full_unstemmed Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title_short Transcriptome profiling of abdominal aortic tissues reveals alterations in mRNAs of Takayasu arteritis
title_sort transcriptome profiling of abdominal aortic tissues reveals alterations in mrnas of takayasu arteritis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709398/
https://www.ncbi.nlm.nih.gov/pubmed/36468014
http://dx.doi.org/10.3389/fgene.2022.1036233
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