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Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children

PURPOSE: Studies have shown that Mycoplasma pneumoniae (Mp) infection can aggravate symptoms in asthmatics. However, the mechanism by which Mp infection exacerbates asthma remains unclear. Metabolomics can help identify the mechanism of Mp aggravating asthma in children, thereby providing more a pot...

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Autores principales: Luo, Jiaying, Chen, Huian, Zhang, Qiyong, Huang, Xinyun, Qin, Xu, Li, Jing, Chen, Siyi, Xiao, Yongxin, Sun, Lihong, Sun, Baoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709688/
https://www.ncbi.nlm.nih.gov/pubmed/36426399
http://dx.doi.org/10.4168/aair.2022.14.6.713
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author Luo, Jiaying
Chen, Huian
Zhang, Qiyong
Huang, Xinyun
Qin, Xu
Li, Jing
Chen, Siyi
Xiao, Yongxin
Sun, Lihong
Sun, Baoqing
author_facet Luo, Jiaying
Chen, Huian
Zhang, Qiyong
Huang, Xinyun
Qin, Xu
Li, Jing
Chen, Siyi
Xiao, Yongxin
Sun, Lihong
Sun, Baoqing
author_sort Luo, Jiaying
collection PubMed
description PURPOSE: Studies have shown that Mycoplasma pneumoniae (Mp) infection can aggravate symptoms in asthmatics. However, the mechanism by which Mp infection exacerbates asthma remains unclear. Metabolomics can help identify the mechanism of Mp aggravating asthma in children, thereby providing more a potential target for improving clinical treatment programs. In this article, we analyzed the metabolic level of patients to explain how Mp aggravates asthma in children. METHODS: We divided the subjects into the asthma, Mp infection, asthma combined with Mp infection and healthy groups. Patients’ peripheral blood was collected for metabolic and interaction analysis. Cytokine levels were measured via serum and exhaled breath condensate (EBC). RESULTS: A total of 150 participating subjects were divided into four groups after exclusion. We found out that there were different metabolic pathways between the healthy and disease groups. The major pathways of both asthma and asthma combined with Mp infection were valine, leucine and isoleucine biosynthesis; malate-aspartate shuttle was the main differential pathway for Mp infection. Moreover, even though three disease groups involved 81 metabolites at the same time, compared with asthma combined with Mp infection, 2 single disease groups still involved different amino acid pathways (phenylalanine, tyrosine and tryptophan biosynthesis; valine, leucine and isoleucine biosynthesis). Interaction analysis showed that Mp infection in asthmatic patients not only activated cytokines, but also activated Toll-like receptors (TLRs) 2 and 6. Finally, the levels of interleukin (IL)-4, IL-8, IL-13 and tumor necrosis factor-α in EBC with asthma combined with Mp infection were significantly higher than the 2 single disease groups. CONCLUSIONS: Mp infection in asthmatic children can cause changes in the levels of various amino acids in the body, which were enriched in the pathways such as valine, leucine and isoleucine biosynthesis. Palmitic acid can activate TLR2, and iloprost reduces IL-10 levels, ultimately leading to the increased airway inflammation.
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spelling pubmed-97096882022-12-16 Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children Luo, Jiaying Chen, Huian Zhang, Qiyong Huang, Xinyun Qin, Xu Li, Jing Chen, Siyi Xiao, Yongxin Sun, Lihong Sun, Baoqing Allergy Asthma Immunol Res Original Article PURPOSE: Studies have shown that Mycoplasma pneumoniae (Mp) infection can aggravate symptoms in asthmatics. However, the mechanism by which Mp infection exacerbates asthma remains unclear. Metabolomics can help identify the mechanism of Mp aggravating asthma in children, thereby providing more a potential target for improving clinical treatment programs. In this article, we analyzed the metabolic level of patients to explain how Mp aggravates asthma in children. METHODS: We divided the subjects into the asthma, Mp infection, asthma combined with Mp infection and healthy groups. Patients’ peripheral blood was collected for metabolic and interaction analysis. Cytokine levels were measured via serum and exhaled breath condensate (EBC). RESULTS: A total of 150 participating subjects were divided into four groups after exclusion. We found out that there were different metabolic pathways between the healthy and disease groups. The major pathways of both asthma and asthma combined with Mp infection were valine, leucine and isoleucine biosynthesis; malate-aspartate shuttle was the main differential pathway for Mp infection. Moreover, even though three disease groups involved 81 metabolites at the same time, compared with asthma combined with Mp infection, 2 single disease groups still involved different amino acid pathways (phenylalanine, tyrosine and tryptophan biosynthesis; valine, leucine and isoleucine biosynthesis). Interaction analysis showed that Mp infection in asthmatic patients not only activated cytokines, but also activated Toll-like receptors (TLRs) 2 and 6. Finally, the levels of interleukin (IL)-4, IL-8, IL-13 and tumor necrosis factor-α in EBC with asthma combined with Mp infection were significantly higher than the 2 single disease groups. CONCLUSIONS: Mp infection in asthmatic children can cause changes in the levels of various amino acids in the body, which were enriched in the pathways such as valine, leucine and isoleucine biosynthesis. Palmitic acid can activate TLR2, and iloprost reduces IL-10 levels, ultimately leading to the increased airway inflammation. The Korean Academy of Asthma, Allergy and Clinical Immunology; The Korean Academy of Pediatric Allergy and Respiratory Disease 2022-10-12 /pmc/articles/PMC9709688/ /pubmed/36426399 http://dx.doi.org/10.4168/aair.2022.14.6.713 Text en Copyright © 2022 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Luo, Jiaying
Chen, Huian
Zhang, Qiyong
Huang, Xinyun
Qin, Xu
Li, Jing
Chen, Siyi
Xiao, Yongxin
Sun, Lihong
Sun, Baoqing
Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title_full Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title_fullStr Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title_full_unstemmed Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title_short Metabolism Characteristics of Mycoplasma pneumoniae Infection in Asthmatic Children
title_sort metabolism characteristics of mycoplasma pneumoniae infection in asthmatic children
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709688/
https://www.ncbi.nlm.nih.gov/pubmed/36426399
http://dx.doi.org/10.4168/aair.2022.14.6.713
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