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Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymeras...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709700/ https://www.ncbi.nlm.nih.gov/pubmed/36467291 http://dx.doi.org/10.1093/ofid/ofac619 |
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| author | Su, Maxwell Ping, Sara Nguyen, Phuong-Vi Rojas, Alejandra Hussaini, Laila Carmola, Ludy Registre Taz, Azmain Sullivan, Julie Martin, Greg S Piantadosi, Anne Martinez, Magaly Lam, Wilbur A Anderson, Evan J Waggoner, Jesse J |
| author_facet | Su, Maxwell Ping, Sara Nguyen, Phuong-Vi Rojas, Alejandra Hussaini, Laila Carmola, Ludy Registre Taz, Azmain Sullivan, Julie Martin, Greg S Piantadosi, Anne Martinez, Magaly Lam, Wilbur A Anderson, Evan J Waggoner, Jesse J |
| author_sort | Su, Maxwell |
| collection | PubMed |
| description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant. |
| format | Online Article Text |
| id | pubmed-9709700 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Oxford University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-97097002022-12-01 Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants Su, Maxwell Ping, Sara Nguyen, Phuong-Vi Rojas, Alejandra Hussaini, Laila Carmola, Ludy Registre Taz, Azmain Sullivan, Julie Martin, Greg S Piantadosi, Anne Martinez, Magaly Lam, Wilbur A Anderson, Evan J Waggoner, Jesse J Open Forum Infect Dis Brief Report Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant. Oxford University Press 2022-11-10 /pmc/articles/PMC9709700/ /pubmed/36467291 http://dx.doi.org/10.1093/ofid/ofac619 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| spellingShingle | Brief Report Su, Maxwell Ping, Sara Nguyen, Phuong-Vi Rojas, Alejandra Hussaini, Laila Carmola, Ludy Registre Taz, Azmain Sullivan, Julie Martin, Greg S Piantadosi, Anne Martinez, Magaly Lam, Wilbur A Anderson, Evan J Waggoner, Jesse J Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title | Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title_full | Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title_fullStr | Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title_full_unstemmed | Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title_short | Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants |
| title_sort | subgenomic rna abundance relative to total viral rna among severe acute respiratory syndrome coronavirus 2 variants |
| topic | Brief Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709700/ https://www.ncbi.nlm.nih.gov/pubmed/36467291 http://dx.doi.org/10.1093/ofid/ofac619 |
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