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In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis

INTRODUCTION: This study aimed to evaluate the in vitro effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. METHODS AND...

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Autores principales: Abu Hasna, Amjad, Khoury, Rayana Duarte, Toia, Cassia Cestari, Gonçalves, Glaucia Beatriz, de Andrade, Flaviana Bombarda, Talge Carvalho, Cláudio Antonio, Ribeiro Camargo, Carlos Henrique, Carneiro Valera, Marcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Center for Endodontic Research 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709833/
https://www.ncbi.nlm.nih.gov/pubmed/36704111
http://dx.doi.org/10.22037/iej.v15i4.26865
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author Abu Hasna, Amjad
Khoury, Rayana Duarte
Toia, Cassia Cestari
Gonçalves, Glaucia Beatriz
de Andrade, Flaviana Bombarda
Talge Carvalho, Cláudio Antonio
Ribeiro Camargo, Carlos Henrique
Carneiro Valera, Marcia
author_facet Abu Hasna, Amjad
Khoury, Rayana Duarte
Toia, Cassia Cestari
Gonçalves, Glaucia Beatriz
de Andrade, Flaviana Bombarda
Talge Carvalho, Cláudio Antonio
Ribeiro Camargo, Carlos Henrique
Carneiro Valera, Marcia
author_sort Abu Hasna, Amjad
collection PubMed
description INTRODUCTION: This study aimed to evaluate the in vitro effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. METHODS AND MATERIALS: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture (CFU/mL), the root canals were contaminated with E. faecalis biofilm, instrumented and then medicated according to the experimental groups. Three samples were collected from the root canals: after 21-days of contamination, immediately after the instrumentation and 14-days after the medication according to the experimental groups. The morphology of E. faecalis biofilm on the root canal walls and bacterial cells viability were assessed by means of SEM and CLSM, respectively. One-way ANOVA and Repeated Measures ANOVA tests were used to analyze the obtained data statistically. RESULTS: CFU/mL analysis showed that CH, NAC and NAC+PDT promoted greater antibacterial activity with statistically significant difference compared to saline solution and PDT (P<0.0001). However, saline solution and PDT were statistically similar (P>0.07). Illustrative images by SEM confirmed partially the CFU/mL results. CLSM showed that all groups were effective eliminating E. faecalis except for the saline solution group. CONCLUSIONS: Based on this in vitro study NAC was bactericidal against E. faecalis biofilms regardless PDT stimulation, presenting similar antimicrobial activity to CH.
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spelling pubmed-97098332023-01-25 In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis Abu Hasna, Amjad Khoury, Rayana Duarte Toia, Cassia Cestari Gonçalves, Glaucia Beatriz de Andrade, Flaviana Bombarda Talge Carvalho, Cláudio Antonio Ribeiro Camargo, Carlos Henrique Carneiro Valera, Marcia Iran Endod J Original Article INTRODUCTION: This study aimed to evaluate the in vitro effectiveness of N-acetylcysteine (NAC), photodynamic therapy (PDT) and NAC with supplemental PDT in optimizing the removal of bacteria from infected dentinal tubules of root canals infected with Enterococcus (E.) faecalis biofilm. METHODS AND MATERIALS: Eighty human teeth were randomly divided into 5 groups (n=16) according to the intracanal medication used: saline solution (control); calcium hydroxide (CH); NAC; PDT; NAC+PDT. Ten samples from each group were prepared for microbiological culture analysis (CFU/mL) and were inoculated with E. faecalis suspension for 21 days for biofilm development; the other six samples from each group were prepared for scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) and submitted to a 5-days contamination protocol including eight centrifugation cycles on every other day for dentinal tubules infection. For antimicrobial activity analysis by microbiological culture (CFU/mL), the root canals were contaminated with E. faecalis biofilm, instrumented and then medicated according to the experimental groups. Three samples were collected from the root canals: after 21-days of contamination, immediately after the instrumentation and 14-days after the medication according to the experimental groups. The morphology of E. faecalis biofilm on the root canal walls and bacterial cells viability were assessed by means of SEM and CLSM, respectively. One-way ANOVA and Repeated Measures ANOVA tests were used to analyze the obtained data statistically. RESULTS: CFU/mL analysis showed that CH, NAC and NAC+PDT promoted greater antibacterial activity with statistically significant difference compared to saline solution and PDT (P<0.0001). However, saline solution and PDT were statistically similar (P>0.07). Illustrative images by SEM confirmed partially the CFU/mL results. CLSM showed that all groups were effective eliminating E. faecalis except for the saline solution group. CONCLUSIONS: Based on this in vitro study NAC was bactericidal against E. faecalis biofilms regardless PDT stimulation, presenting similar antimicrobial activity to CH. Iranian Center for Endodontic Research 2020 /pmc/articles/PMC9709833/ /pubmed/36704111 http://dx.doi.org/10.22037/iej.v15i4.26865 Text en https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International.(https://creativecommons.org/licenses/by-nc-sa/4.0/)
spellingShingle Original Article
Abu Hasna, Amjad
Khoury, Rayana Duarte
Toia, Cassia Cestari
Gonçalves, Glaucia Beatriz
de Andrade, Flaviana Bombarda
Talge Carvalho, Cláudio Antonio
Ribeiro Camargo, Carlos Henrique
Carneiro Valera, Marcia
In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title_full In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title_fullStr In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title_full_unstemmed In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title_short In vitro Evaluation of the Antimicrobial Effect of N-acetylcysteine and Photodynamic Therapy on Root Canals Infected with Enterococcus faecalis
title_sort in vitro evaluation of the antimicrobial effect of n-acetylcysteine and photodynamic therapy on root canals infected with enterococcus faecalis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709833/
https://www.ncbi.nlm.nih.gov/pubmed/36704111
http://dx.doi.org/10.22037/iej.v15i4.26865
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