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Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid
PURPOSE: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. METHODS: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, hist...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710187/ https://www.ncbi.nlm.nih.gov/pubmed/36449813 http://dx.doi.org/10.1590/acb370902 |
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author | Zhao, Dan Xiao, Jinli Qiang, Lijuan Deng, Xingwang An, Jingjing Zhang, Qing Zhao, Fang Ma, Jiaxiang Fang, Chao Guan, Guangyu Wu, Yinsheng Xie, Yan |
author_facet | Zhao, Dan Xiao, Jinli Qiang, Lijuan Deng, Xingwang An, Jingjing Zhang, Qing Zhao, Fang Ma, Jiaxiang Fang, Chao Guan, Guangyu Wu, Yinsheng Xie, Yan |
author_sort | Zhao, Dan |
collection | PubMed |
description | PURPOSE: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. METHODS: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. RESULTS: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). CONCLUSIONS: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation. |
format | Online Article Text |
id | pubmed-9710187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
record_format | MEDLINE/PubMed |
spelling | pubmed-97101872022-12-02 Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid Zhao, Dan Xiao, Jinli Qiang, Lijuan Deng, Xingwang An, Jingjing Zhang, Qing Zhao, Fang Ma, Jiaxiang Fang, Chao Guan, Guangyu Wu, Yinsheng Xie, Yan Acta Cir Bras Original Article PURPOSE: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. METHODS: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. RESULTS: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). CONCLUSIONS: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia 2022-11-28 /pmc/articles/PMC9710187/ /pubmed/36449813 http://dx.doi.org/10.1590/acb370902 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zhao, Dan Xiao, Jinli Qiang, Lijuan Deng, Xingwang An, Jingjing Zhang, Qing Zhao, Fang Ma, Jiaxiang Fang, Chao Guan, Guangyu Wu, Yinsheng Xie, Yan Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title | Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_full | Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_fullStr | Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_full_unstemmed | Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_short | Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_sort | walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710187/ https://www.ncbi.nlm.nih.gov/pubmed/36449813 http://dx.doi.org/10.1590/acb370902 |
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