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Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model
INTRODUCTION: In the present study, we aimed to examine the effects of the administration of whey protein through rectal enema to rats with acetic acid-induced ulcerative colitis on the pathways of nuclear-related factor-2 (Nrf-2), haem oxygenase-1 (HO-1), nuclear factor κB (NF-κB), active protein k...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Termedia Publishing House
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710278/ https://www.ncbi.nlm.nih.gov/pubmed/36457969 http://dx.doi.org/10.5114/aoms/105839 |
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author | Tunc, Nurettin Sahin, Abdurahman Demirel, Ulvi Artas, Gokhan Sahin, Kazım Bahcecioglu, İbrahim Halil Yalniz, Mehmet |
author_facet | Tunc, Nurettin Sahin, Abdurahman Demirel, Ulvi Artas, Gokhan Sahin, Kazım Bahcecioglu, İbrahim Halil Yalniz, Mehmet |
author_sort | Tunc, Nurettin |
collection | PubMed |
description | INTRODUCTION: In the present study, we aimed to examine the effects of the administration of whey protein through rectal enema to rats with acetic acid-induced ulcerative colitis on the pathways of nuclear-related factor-2 (Nrf-2), haem oxygenase-1 (HO-1), nuclear factor κB (NF-κB), active protein kinase-1 (AP-1), tumour necrotising factor-α (TNF-α), and cyclooxygenase-2 (COX-2), IL-6, IL-10. MATERIAL AND METHODS: Twenty-eight rats were employed for the trial. Ulcerative colitis was induced through the use of acetic acid. The therapeutic doses of whey protein were administered rectally. Ulcerative colitis was subjected to histopathological examination and protein levels in colon tissue were measured with the Western blot method. RESULTS: The significant increases observed in the levels of AP-1, COX-2, IL-6, IL-10, NF-κB, and TNF-α as markers of inflammation following the development of ulcerative colitis showed remarkable decreases along with the administration of whey protein (p < 0.05). On the other hand, we identified a decrease in the Nrf2-ARE signal pathway and HO-1 protein having protective roles in the colon inflammatory response along with the development of ulcerative colitis and activation of the Nrf2-HO-1 pathway by the whey protein. CONCLUSIONS: Whey protein modulates Nrf2/HO-1 and NF-kB pathways, thereby creating a therapeutic effect against colonic inflammation induced by acetic acid (AA) due to its anti-inflammatory effects. |
format | Online Article Text |
id | pubmed-9710278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-97102782022-11-30 Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model Tunc, Nurettin Sahin, Abdurahman Demirel, Ulvi Artas, Gokhan Sahin, Kazım Bahcecioglu, İbrahim Halil Yalniz, Mehmet Arch Med Sci Experimental Research INTRODUCTION: In the present study, we aimed to examine the effects of the administration of whey protein through rectal enema to rats with acetic acid-induced ulcerative colitis on the pathways of nuclear-related factor-2 (Nrf-2), haem oxygenase-1 (HO-1), nuclear factor κB (NF-κB), active protein kinase-1 (AP-1), tumour necrotising factor-α (TNF-α), and cyclooxygenase-2 (COX-2), IL-6, IL-10. MATERIAL AND METHODS: Twenty-eight rats were employed for the trial. Ulcerative colitis was induced through the use of acetic acid. The therapeutic doses of whey protein were administered rectally. Ulcerative colitis was subjected to histopathological examination and protein levels in colon tissue were measured with the Western blot method. RESULTS: The significant increases observed in the levels of AP-1, COX-2, IL-6, IL-10, NF-κB, and TNF-α as markers of inflammation following the development of ulcerative colitis showed remarkable decreases along with the administration of whey protein (p < 0.05). On the other hand, we identified a decrease in the Nrf2-ARE signal pathway and HO-1 protein having protective roles in the colon inflammatory response along with the development of ulcerative colitis and activation of the Nrf2-HO-1 pathway by the whey protein. CONCLUSIONS: Whey protein modulates Nrf2/HO-1 and NF-kB pathways, thereby creating a therapeutic effect against colonic inflammation induced by acetic acid (AA) due to its anti-inflammatory effects. Termedia Publishing House 2021-03-21 /pmc/articles/PMC9710278/ /pubmed/36457969 http://dx.doi.org/10.5114/aoms/105839 Text en Copyright: © 2021 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Experimental Research Tunc, Nurettin Sahin, Abdurahman Demirel, Ulvi Artas, Gokhan Sahin, Kazım Bahcecioglu, İbrahim Halil Yalniz, Mehmet Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title | Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title_full | Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title_fullStr | Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title_full_unstemmed | Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title_short | Favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
title_sort | favourable effects of whey protein on acetic acid-induced ulcerative colitis in a rat model |
topic | Experimental Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710278/ https://www.ncbi.nlm.nih.gov/pubmed/36457969 http://dx.doi.org/10.5114/aoms/105839 |
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