MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway

INTRODUCTION: In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). METHODS: We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, aju...

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Autores principales: Yang, Wengang, Zhao, Lei, Xue, Song, Liu, Jidong, Shan, Jianggui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2022
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710289/
https://www.ncbi.nlm.nih.gov/pubmed/36457985
http://dx.doi.org/10.5114/aoms/154956
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author Yang, Wengang
Zhao, Lei
Xue, Song
Liu, Jidong
Shan, Jianggui
author_facet Yang, Wengang
Zhao, Lei
Xue, Song
Liu, Jidong
Shan, Jianggui
author_sort Yang, Wengang
collection PubMed
description INTRODUCTION: In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). METHODS: We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups. RESULTS: MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells. CONCLUSIONS: MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway.
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spelling pubmed-97102892022-11-30 MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway Yang, Wengang Zhao, Lei Xue, Song Liu, Jidong Shan, Jianggui Arch Med Sci Research Letter INTRODUCTION: In this study, we aimed to investigate the role of miRNA-21 and the regulating pathway that promotes the differentiation of bone marrow mesenchymal stem cells (BMSCs). METHODS: We used miR-21-OE, miR-21-KD, ajuba-OE and ajuba-KD plasmids to infect BMSCs. The expression of miRNA-21, ajuba, Isl1 and cTnI was detected by RT-qPCR, WB and immunofluorescence staining in groups. RESULTS: MiRNA-21 over-expression increased the expression of Isl1, and vice versa. Ajuba over-expression decreased the expression of Isl1, and vice versa. Ajuba negatively regulated the differentiation of BMSCs into cardiomyocyte-like cells. CONCLUSIONS: MiRNA-21 could regulate differentiation of bone marrow mesenchymal stem cells (BMSCs) to cardiomyocyte-like cells through the ajuba/Isl1 axis pathway. Termedia Publishing House 2022-11-07 /pmc/articles/PMC9710289/ /pubmed/36457985 http://dx.doi.org/10.5114/aoms/154956 Text en Copyright: © 2022 Termedia & Banach https://creativecommons.org/licenses/by-nc-sa/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
spellingShingle Research Letter
Yang, Wengang
Zhao, Lei
Xue, Song
Liu, Jidong
Shan, Jianggui
MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title_full MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title_fullStr MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title_full_unstemmed MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title_short MiRNA-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the Ajuba/Isl1 axis pathway
title_sort mirna-21 promotes differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells by regulating the ajuba/isl1 axis pathway
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710289/
https://www.ncbi.nlm.nih.gov/pubmed/36457985
http://dx.doi.org/10.5114/aoms/154956
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