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Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition

Due to different nucleotide preferences at target sites, no single Cas9 is capable of editing all sequences. Thus, this highlights the need to establish a Cas9 repertoire covering all sequences for efficient genome editing. Cas9s with simple protospacer adjacent motif (PAM) requirements are particul...

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Detalles Bibliográficos
Autores principales:	Wang, Shuai, Tao, Chen, Mao, Huilin, Hou, Linghui, Wang, Yao, Qi, Tao, Yang, Yuan, Ong, Sang-Ging, Hu, Shijun, Chai, Renjie, Wang, Yongming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9710800/ https://www.ncbi.nlm.nih.gov/pubmed/36449487 http://dx.doi.org/10.1371/journal.pbio.3001897

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