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The mechanosensitive ion channel PIEZO1 promotes satellite cell function in muscle regeneration

Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. After skeletal muscle injury, quiescent MuSCs are activated to enter the cell cycle and proliferate, thereby initiating regeneration; however, the mechanisms that ensure successful...

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Detalles Bibliográficos
Autores principales: Hirano, Kotaro, Tsuchiya, Masaki, Shiomi, Akifumi, Takabayashi, Seiji, Suzuki, Miki, Ishikawa, Yudai, Kawano, Yuya, Takabayashi, Yutaka, Nishikawa, Kaori, Nagao, Kohjiro, Umemoto, Eiji, Kitajima, Yasuo, Ono, Yusuke, Nonomura, Keiko, Shintaku, Hirofumi, Mori, Yasuo, Umeda, Masato, Hara, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9711862/
https://www.ncbi.nlm.nih.gov/pubmed/36446523
http://dx.doi.org/10.26508/lsa.202201783
Descripción
Sumario:Muscle satellite cells (MuSCs), myogenic stem cells in skeletal muscles, play an essential role in muscle regeneration. After skeletal muscle injury, quiescent MuSCs are activated to enter the cell cycle and proliferate, thereby initiating regeneration; however, the mechanisms that ensure successful MuSC division, including chromosome segregation, remain unclear. Here, we show that PIEZO1, a calcium ion (Ca(2+))-permeable cation channel activated by membrane tension, mediates spontaneous Ca(2+) influx to control the regenerative function of MuSCs. Our genetic engineering approach in mice revealed that PIEZO1 is functionally expressed in MuSCs and that Piezo1 deletion in these cells delays myofibre regeneration after injury. These results are, at least in part, due to a mitotic defect in MuSCs. Mechanistically, this phenotype is caused by impaired PIEZO1-Rho signalling during myogenesis. Thus, we provide the first concrete evidence that PIEZO1, a bona fide mechanosensitive ion channel, promotes proliferation and regenerative functions of MuSCs through precise control of cell division.