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Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?

Background Lamotrigine (LTG) and subconvulsive doses of pentylenetetrazol (PTZ) as a model mimic drug-resistant epilepsy (DRE), which is a serious unmet medical condition. Previous evidence suggests an imperative role of neuroinflammation in the development of DRE. Various preclinical models of brai...

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Autores principales: Thapliyal, Surabhi, Singh, Jagjit, Mamgain, Mukesh, Kumar, Ashish, Bisht, Manisha, Singh, Ashok, Meena, Kiran, Kishore, Sanjeev, Handu, Shailendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cureus 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9711917/
https://www.ncbi.nlm.nih.gov/pubmed/36465733
http://dx.doi.org/10.7759/cureus.30892
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author Thapliyal, Surabhi
Singh, Jagjit
Mamgain, Mukesh
Kumar, Ashish
Bisht, Manisha
Singh, Ashok
Meena, Kiran
Kishore, Sanjeev
Handu, Shailendra
author_facet Thapliyal, Surabhi
Singh, Jagjit
Mamgain, Mukesh
Kumar, Ashish
Bisht, Manisha
Singh, Ashok
Meena, Kiran
Kishore, Sanjeev
Handu, Shailendra
author_sort Thapliyal, Surabhi
collection PubMed
description Background Lamotrigine (LTG) and subconvulsive doses of pentylenetetrazol (PTZ) as a model mimic drug-resistant epilepsy (DRE), which is a serious unmet medical condition. Previous evidence suggests an imperative role of neuroinflammation in the development of DRE. Various preclinical models of brain injury have reported potent anti-inflammatory and antioxidant properties of ferulic acid (FA). Therefore, its efficacy against intractable epilepsy is worthwhile to study. Materials and methods The present study evaluated the efficacy of FA in LTG and PTZ-induced refractory seizures in mice. On every alternate day for 38 days, LTG (5mg/kg) was injected before PTZ (30-40mg/kg) to establish a murine model of DRE. Animals were treated with two doses of FA (40, 80 mg/kg). All the animals were assessed for seizure score and the latency of seizures every alternate day till the end of the study. Histopathological score and the levels of pro-inflammatory mediators, interleukin-1βeta (IL-Iβ), tumor necrosis factor-alpha (TNF-α), and matrix metalloproteinase-9 (MMP-9) were quantified in the brain tissue of these mice.  Results Ferulic acid (FA) neither decreases the LTG and PTZ-induced refractory seizures score nor increases the latency to develop seizures. In addition, the injury to hippocampal neurons and the levels of pro-inflammatory cytokines were comparable with two doses of FA in treated mice. Conclusion In the present study, single-dose FA treatment does not show any beneficial effect against the LTG/PTZ model of DRE. Therefore, its single-dose administration might not be beneficial against the DRE model.
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spelling pubmed-97119172022-12-02 Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not? Thapliyal, Surabhi Singh, Jagjit Mamgain, Mukesh Kumar, Ashish Bisht, Manisha Singh, Ashok Meena, Kiran Kishore, Sanjeev Handu, Shailendra Cureus Neurology Background Lamotrigine (LTG) and subconvulsive doses of pentylenetetrazol (PTZ) as a model mimic drug-resistant epilepsy (DRE), which is a serious unmet medical condition. Previous evidence suggests an imperative role of neuroinflammation in the development of DRE. Various preclinical models of brain injury have reported potent anti-inflammatory and antioxidant properties of ferulic acid (FA). Therefore, its efficacy against intractable epilepsy is worthwhile to study. Materials and methods The present study evaluated the efficacy of FA in LTG and PTZ-induced refractory seizures in mice. On every alternate day for 38 days, LTG (5mg/kg) was injected before PTZ (30-40mg/kg) to establish a murine model of DRE. Animals were treated with two doses of FA (40, 80 mg/kg). All the animals were assessed for seizure score and the latency of seizures every alternate day till the end of the study. Histopathological score and the levels of pro-inflammatory mediators, interleukin-1βeta (IL-Iβ), tumor necrosis factor-alpha (TNF-α), and matrix metalloproteinase-9 (MMP-9) were quantified in the brain tissue of these mice.  Results Ferulic acid (FA) neither decreases the LTG and PTZ-induced refractory seizures score nor increases the latency to develop seizures. In addition, the injury to hippocampal neurons and the levels of pro-inflammatory cytokines were comparable with two doses of FA in treated mice. Conclusion In the present study, single-dose FA treatment does not show any beneficial effect against the LTG/PTZ model of DRE. Therefore, its single-dose administration might not be beneficial against the DRE model. Cureus 2022-10-31 /pmc/articles/PMC9711917/ /pubmed/36465733 http://dx.doi.org/10.7759/cureus.30892 Text en Copyright © 2022, Thapliyal et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Neurology
Thapliyal, Surabhi
Singh, Jagjit
Mamgain, Mukesh
Kumar, Ashish
Bisht, Manisha
Singh, Ashok
Meena, Kiran
Kishore, Sanjeev
Handu, Shailendra
Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title_full Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title_fullStr Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title_full_unstemmed Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title_short Efficacy of Ferulic Acid in an Animal Model of Drug-Resistant Epilepsy: Beneficial or Not?
title_sort efficacy of ferulic acid in an animal model of drug-resistant epilepsy: beneficial or not?
topic Neurology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9711917/
https://www.ncbi.nlm.nih.gov/pubmed/36465733
http://dx.doi.org/10.7759/cureus.30892
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