Cargando…

Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa

In cystic fibrosis individuals, chronic lung infections and hospital-acquired pneumonia are caused by Pseudomonas aeruginosa. P. aeruginosa generates siderophores such as pyoverdine (PVD) as iron uptake systems to cover its needs of iron ions for growth and infection. lasR quorum sensing (QS) gene h...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamza, Esraa H., El-Shawadfy, Ashraf M., Allam, Ayman A., Hassanein, Wesam A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712133/
https://www.ncbi.nlm.nih.gov/pubmed/36466220
http://dx.doi.org/10.1016/j.sjbs.2022.103492
_version_ 1784841733224267776
author Hamza, Esraa H.
El-Shawadfy, Ashraf M.
Allam, Ayman A.
Hassanein, Wesam A.
author_facet Hamza, Esraa H.
El-Shawadfy, Ashraf M.
Allam, Ayman A.
Hassanein, Wesam A.
author_sort Hamza, Esraa H.
collection PubMed
description In cystic fibrosis individuals, chronic lung infections and hospital-acquired pneumonia are caused by Pseudomonas aeruginosa. P. aeruginosa generates siderophores such as pyoverdine (PVD) as iron uptake systems to cover its needs of iron ions for growth and infection. lasR quorum sensing (QS) gene has a crucial function in PVD production and biofilm generation in P. aeruginosa. Fifty isolates of P. aeruginosa were obtained from clinical specimens of sputum (collected from individuals suffering from pulmonary infections). Antibiotic sensitivity test was performed for 50P. aeruginosa isolates by using 10 different types of antibiotics. All isolates of P. aeruginosa showed resistance for all 10 using antibiotics in this study. Ten multidrug resistant isoloates of P. aeruginosa were selected for next tests. Virulence factors of ten multidrug resistant isolates of P. aeruginosa, such as biofilm generation, PVD production, and lasR gene were detected. From results, all 10P. aeruginosa isolates can produce biofilm, PVD, and contain lasR gene. The produced amplicon for the lasR gene was 725 bp. After mice injection by fresh and heated PVD produced by P. aeruginosa PS10 LC619328.2, the fresh PVD caused 100 % mortality within five days using 0.3 ml of its concentration (37.4 µM), while (15.3 µM) of heated PVD (toxoid) caused 50 % mortality.
format Online
Article
Text
id pubmed-9712133
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-97121332022-12-02 Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa Hamza, Esraa H. El-Shawadfy, Ashraf M. Allam, Ayman A. Hassanein, Wesam A. Saudi J Biol Sci Original Article In cystic fibrosis individuals, chronic lung infections and hospital-acquired pneumonia are caused by Pseudomonas aeruginosa. P. aeruginosa generates siderophores such as pyoverdine (PVD) as iron uptake systems to cover its needs of iron ions for growth and infection. lasR quorum sensing (QS) gene has a crucial function in PVD production and biofilm generation in P. aeruginosa. Fifty isolates of P. aeruginosa were obtained from clinical specimens of sputum (collected from individuals suffering from pulmonary infections). Antibiotic sensitivity test was performed for 50P. aeruginosa isolates by using 10 different types of antibiotics. All isolates of P. aeruginosa showed resistance for all 10 using antibiotics in this study. Ten multidrug resistant isoloates of P. aeruginosa were selected for next tests. Virulence factors of ten multidrug resistant isolates of P. aeruginosa, such as biofilm generation, PVD production, and lasR gene were detected. From results, all 10P. aeruginosa isolates can produce biofilm, PVD, and contain lasR gene. The produced amplicon for the lasR gene was 725 bp. After mice injection by fresh and heated PVD produced by P. aeruginosa PS10 LC619328.2, the fresh PVD caused 100 % mortality within five days using 0.3 ml of its concentration (37.4 µM), while (15.3 µM) of heated PVD (toxoid) caused 50 % mortality. Elsevier 2023-01 2022-11-12 /pmc/articles/PMC9712133/ /pubmed/36466220 http://dx.doi.org/10.1016/j.sjbs.2022.103492 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Hamza, Esraa H.
El-Shawadfy, Ashraf M.
Allam, Ayman A.
Hassanein, Wesam A.
Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title_full Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title_fullStr Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title_full_unstemmed Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title_short Study on pyoverdine and biofilm production with detection of LasR gene in MDR Pseudomonas aeruginosa
title_sort study on pyoverdine and biofilm production with detection of lasr gene in mdr pseudomonas aeruginosa
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712133/
https://www.ncbi.nlm.nih.gov/pubmed/36466220
http://dx.doi.org/10.1016/j.sjbs.2022.103492
work_keys_str_mv AT hamzaesraah studyonpyoverdineandbiofilmproductionwithdetectionoflasrgeneinmdrpseudomonasaeruginosa
AT elshawadfyashrafm studyonpyoverdineandbiofilmproductionwithdetectionoflasrgeneinmdrpseudomonasaeruginosa
AT allamaymana studyonpyoverdineandbiofilmproductionwithdetectionoflasrgeneinmdrpseudomonasaeruginosa
AT hassaneinwesama studyonpyoverdineandbiofilmproductionwithdetectionoflasrgeneinmdrpseudomonasaeruginosa