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In vitro and in vivo models for monkeypox

The emergence and rapid spread outside of monkeypox virus (MPXV) to non-endemic areas has led to another global health emergency in the midst of the COVID-19 pandemic. The scientific community has sought to rapidly develop in vitro and in vivo models that could be applied in research with MPXV. In v...

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Autores principales: Rosa, Rafael Borges, Ferreira de Castro, Emilene, Vieira da Silva, Murilo, Paiva Ferreira, Denise Caroline, Jardim, Ana Carolina Gomes, Santos, Igor Andrade, Marinho, Mikaela dos Santos, Ferreira França, Flávia Batista, Pena, Lindomar José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712139/
https://www.ncbi.nlm.nih.gov/pubmed/36471873
http://dx.doi.org/10.1016/j.isci.2022.105702
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author Rosa, Rafael Borges
Ferreira de Castro, Emilene
Vieira da Silva, Murilo
Paiva Ferreira, Denise Caroline
Jardim, Ana Carolina Gomes
Santos, Igor Andrade
Marinho, Mikaela dos Santos
Ferreira França, Flávia Batista
Pena, Lindomar José
author_facet Rosa, Rafael Borges
Ferreira de Castro, Emilene
Vieira da Silva, Murilo
Paiva Ferreira, Denise Caroline
Jardim, Ana Carolina Gomes
Santos, Igor Andrade
Marinho, Mikaela dos Santos
Ferreira França, Flávia Batista
Pena, Lindomar José
author_sort Rosa, Rafael Borges
collection PubMed
description The emergence and rapid spread outside of monkeypox virus (MPXV) to non-endemic areas has led to another global health emergency in the midst of the COVID-19 pandemic. The scientific community has sought to rapidly develop in vitro and in vivo models that could be applied in research with MPXV. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures. In vitro models are considered cost-effective and can be done in highly controlled conditions; however, they do not always resemble physiological conditions. In this way, several in vivo models are being characterized to meet the growing demand for new studies related to MPXV. In this review, we summarize the main MPXV models that have already been developed and discuss how they can contribute to advance the understanding of its pathogenesis, replication, and transmission, as well as identifying antivirals to treat infected patients.
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spelling pubmed-97121392022-12-01 In vitro and in vivo models for monkeypox Rosa, Rafael Borges Ferreira de Castro, Emilene Vieira da Silva, Murilo Paiva Ferreira, Denise Caroline Jardim, Ana Carolina Gomes Santos, Igor Andrade Marinho, Mikaela dos Santos Ferreira França, Flávia Batista Pena, Lindomar José iScience Review The emergence and rapid spread outside of monkeypox virus (MPXV) to non-endemic areas has led to another global health emergency in the midst of the COVID-19 pandemic. The scientific community has sought to rapidly develop in vitro and in vivo models that could be applied in research with MPXV. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures. In vitro models are considered cost-effective and can be done in highly controlled conditions; however, they do not always resemble physiological conditions. In this way, several in vivo models are being characterized to meet the growing demand for new studies related to MPXV. In this review, we summarize the main MPXV models that have already been developed and discuss how they can contribute to advance the understanding of its pathogenesis, replication, and transmission, as well as identifying antivirals to treat infected patients. Elsevier 2022-12-01 /pmc/articles/PMC9712139/ /pubmed/36471873 http://dx.doi.org/10.1016/j.isci.2022.105702 Text en © 2022. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Review
Rosa, Rafael Borges
Ferreira de Castro, Emilene
Vieira da Silva, Murilo
Paiva Ferreira, Denise Caroline
Jardim, Ana Carolina Gomes
Santos, Igor Andrade
Marinho, Mikaela dos Santos
Ferreira França, Flávia Batista
Pena, Lindomar José
In vitro and in vivo models for monkeypox
title In vitro and in vivo models for monkeypox
title_full In vitro and in vivo models for monkeypox
title_fullStr In vitro and in vivo models for monkeypox
title_full_unstemmed In vitro and in vivo models for monkeypox
title_short In vitro and in vivo models for monkeypox
title_sort in vitro and in vivo models for monkeypox
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712139/
https://www.ncbi.nlm.nih.gov/pubmed/36471873
http://dx.doi.org/10.1016/j.isci.2022.105702
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