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Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus

Background: Irreversible electroporation (IRE) has been investigated in the alimentary tract; however, the lack of dedicated electrodes and insufficient tissue responses made its application limited. The aim of this study was to investigate the efficacy and safety of image-guided stent-directed IRE...

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Autores principales: Kim, Song Hee, Jeong, Seung, Kang, Jeon Min, Park, Yubeen, Ryu, Dae Sung, Won, Dong-Sung, Kim, Ji Won, Zeng, Chu Hui, Kim, Hyung-Sik, Kim, Hong Bae, Lee, Sang Soo, Park, Jung-Hoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712177/
https://www.ncbi.nlm.nih.gov/pubmed/36466325
http://dx.doi.org/10.3389/fbioe.2022.934858
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author Kim, Song Hee
Jeong, Seung
Kang, Jeon Min
Park, Yubeen
Ryu, Dae Sung
Won, Dong-Sung
Kim, Ji Won
Zeng, Chu Hui
Kim, Hyung-Sik
Kim, Hong Bae
Lee, Sang Soo
Park, Jung-Hoon
author_facet Kim, Song Hee
Jeong, Seung
Kang, Jeon Min
Park, Yubeen
Ryu, Dae Sung
Won, Dong-Sung
Kim, Ji Won
Zeng, Chu Hui
Kim, Hyung-Sik
Kim, Hong Bae
Lee, Sang Soo
Park, Jung-Hoon
author_sort Kim, Song Hee
collection PubMed
description Background: Irreversible electroporation (IRE) has been investigated in the alimentary tract; however, the lack of dedicated electrodes and insufficient tissue responses made its application limited. The aim of this study was to investigate the efficacy and safety of image-guided stent-directed IRE in the rat esophagus. Methods: The bipolar self-expandable electrode (SE) was developed using the braiding technique. A finite element analysis was performed to validate optimal electrical field strength for the rat esophagus. A total of 40 out of 50 rats received stent-directed IRE and were sacrificed at 10 h, 3 days, 7 days, and 28 days of 10 each. The remaining ten rats underwent a sham procedure. The outcomes of stent-directed IRE were assessed by esophagography and histological responses. Results: Stent-directed IRE was technically successful in all rats with mild muscle contraction. The heart rate dropped immediately and gradually recovered at 180 s. TUNEL and caspase-3 with submucosal thickness significantly increased at 10 h and Day 3 compared with those of the sham control (all p < 0.001). The thickness of epithelial layers with collagen deposition significantly decreased at 10 h and Day 3 (all p < 0.001), however, increased at Day 7 compared with that of the sham control (all p < 0.05). The Ki67-positive deposition significantly increased at Day 3 and 7 compared with that of the sham control (all p < 0.001). All variables were similar to those of the sham control at Day 28. Conclusion: Image-guided stent-directed IRE was effective and safe in the rat esophagus. It seems to have effectively and evenly induced cell death and gradually recovered with cellular regeneration.
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spelling pubmed-97121772022-12-02 Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus Kim, Song Hee Jeong, Seung Kang, Jeon Min Park, Yubeen Ryu, Dae Sung Won, Dong-Sung Kim, Ji Won Zeng, Chu Hui Kim, Hyung-Sik Kim, Hong Bae Lee, Sang Soo Park, Jung-Hoon Front Bioeng Biotechnol Bioengineering and Biotechnology Background: Irreversible electroporation (IRE) has been investigated in the alimentary tract; however, the lack of dedicated electrodes and insufficient tissue responses made its application limited. The aim of this study was to investigate the efficacy and safety of image-guided stent-directed IRE in the rat esophagus. Methods: The bipolar self-expandable electrode (SE) was developed using the braiding technique. A finite element analysis was performed to validate optimal electrical field strength for the rat esophagus. A total of 40 out of 50 rats received stent-directed IRE and were sacrificed at 10 h, 3 days, 7 days, and 28 days of 10 each. The remaining ten rats underwent a sham procedure. The outcomes of stent-directed IRE were assessed by esophagography and histological responses. Results: Stent-directed IRE was technically successful in all rats with mild muscle contraction. The heart rate dropped immediately and gradually recovered at 180 s. TUNEL and caspase-3 with submucosal thickness significantly increased at 10 h and Day 3 compared with those of the sham control (all p < 0.001). The thickness of epithelial layers with collagen deposition significantly decreased at 10 h and Day 3 (all p < 0.001), however, increased at Day 7 compared with that of the sham control (all p < 0.05). The Ki67-positive deposition significantly increased at Day 3 and 7 compared with that of the sham control (all p < 0.001). All variables were similar to those of the sham control at Day 28. Conclusion: Image-guided stent-directed IRE was effective and safe in the rat esophagus. It seems to have effectively and evenly induced cell death and gradually recovered with cellular regeneration. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9712177/ /pubmed/36466325 http://dx.doi.org/10.3389/fbioe.2022.934858 Text en Copyright © 2022 Kim, Jeong, Kang, Park, Ryu, Won, Kim, Zeng, Kim, Kim, Lee and Park. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Kim, Song Hee
Jeong, Seung
Kang, Jeon Min
Park, Yubeen
Ryu, Dae Sung
Won, Dong-Sung
Kim, Ji Won
Zeng, Chu Hui
Kim, Hyung-Sik
Kim, Hong Bae
Lee, Sang Soo
Park, Jung-Hoon
Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title_full Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title_fullStr Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title_full_unstemmed Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title_short Image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
title_sort image-guided stent-directed irreversible electroporation for circumferential ablation in the rat esophagus
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712177/
https://www.ncbi.nlm.nih.gov/pubmed/36466325
http://dx.doi.org/10.3389/fbioe.2022.934858
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