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A multiple detection method for distinguishing gene mutations based on melting curves of extended quenching probes
Conventional PCR methods can detect only a few targets simultaneously and do not fulfill most clinical requirements, especially those for detecting plasma circulating DNA. By designing characteristic universal fluorescent probes, combining multiplex PCR with the invasive reaction, and analyzing the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712562/ https://www.ncbi.nlm.nih.gov/pubmed/36468116 http://dx.doi.org/10.1016/j.heliyon.2022.e11856 |