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The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance

Insects are the largest group of arthropod phyla and are capable of surviving in a variety of environments. One of the most important factors in enabling them to do so is their resistance to temperature stress, i.e., cold tolerance. The neuroendocrine system, together with the immune system, coopera...

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Autores principales: Lubawy, Jan, Hornik, Justyna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712581/
https://www.ncbi.nlm.nih.gov/pubmed/36450889
http://dx.doi.org/10.1038/s41598-022-25235-w
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author Lubawy, Jan
Hornik, Justyna
author_facet Lubawy, Jan
Hornik, Justyna
author_sort Lubawy, Jan
collection PubMed
description Insects are the largest group of arthropod phyla and are capable of surviving in a variety of environments. One of the most important factors in enabling them to do so is their resistance to temperature stress, i.e., cold tolerance. The neuroendocrine system, together with the immune system, cooperates to regulate a number of physiological processes that are essential for the stability of the organism in stressful conditions. However, to date, no one has studied the effect of insect myoinhibitory peptides (MIPs) on cold stress tolerance and immune system activity. Here, we investigated the effect of Tenmo-MIP 5 (10(–6) M), cold stress (− 5 °C) and a combination of both on the immune response of Tenebrio molitor. All three treatments caused upregulation of immune-related genes (antimicrobial peptides and Toll) and increased phagocytosis activity (by approximately 10%). However, phenoloxidase activity and mortality were increased only after peptide injection and the combination of both treatments. The peptide injection combined with cold stress caused 40% higher mortality than that in the control. Together, our results show the links between cold stress, MIPs activity and the immune response, and to our knowledge, this is the first report showing the effect of MIP on the insect immune system.
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spelling pubmed-97125812022-12-02 The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance Lubawy, Jan Hornik, Justyna Sci Rep Article Insects are the largest group of arthropod phyla and are capable of surviving in a variety of environments. One of the most important factors in enabling them to do so is their resistance to temperature stress, i.e., cold tolerance. The neuroendocrine system, together with the immune system, cooperates to regulate a number of physiological processes that are essential for the stability of the organism in stressful conditions. However, to date, no one has studied the effect of insect myoinhibitory peptides (MIPs) on cold stress tolerance and immune system activity. Here, we investigated the effect of Tenmo-MIP 5 (10(–6) M), cold stress (− 5 °C) and a combination of both on the immune response of Tenebrio molitor. All three treatments caused upregulation of immune-related genes (antimicrobial peptides and Toll) and increased phagocytosis activity (by approximately 10%). However, phenoloxidase activity and mortality were increased only after peptide injection and the combination of both treatments. The peptide injection combined with cold stress caused 40% higher mortality than that in the control. Together, our results show the links between cold stress, MIPs activity and the immune response, and to our knowledge, this is the first report showing the effect of MIP on the insect immune system. Nature Publishing Group UK 2022-11-30 /pmc/articles/PMC9712581/ /pubmed/36450889 http://dx.doi.org/10.1038/s41598-022-25235-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lubawy, Jan
Hornik, Justyna
The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title_full The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title_fullStr The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title_full_unstemmed The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title_short The effect of B-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
title_sort effect of b-type allatostatin neuropeptides on crosstalk between the insect immune response and cold tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9712581/
https://www.ncbi.nlm.nih.gov/pubmed/36450889
http://dx.doi.org/10.1038/s41598-022-25235-w
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