Cargando…

Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains

Toxoplasmosis is one of the most common zoonotic diseases in the world. Felines excrete Toxoplasma gondii oocysts, which play a key role in the transmission of this protozoon. Pathological diagnoses were performed on four carcasses of captive tigers collected from 2019 to 2021 in China, and T. gondi...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Hongjie, Yang, Liulu, Zhu, Niuping, Li, Junbao, Su, Chunlei, Jiang, Yibao, Yang, Yurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713289/
https://www.ncbi.nlm.nih.gov/pubmed/36467851
http://dx.doi.org/10.1016/j.ijppaw.2022.11.009
_version_ 1784841986935619584
author Ren, Hongjie
Yang, Liulu
Zhu, Niuping
Li, Junbao
Su, Chunlei
Jiang, Yibao
Yang, Yurong
author_facet Ren, Hongjie
Yang, Liulu
Zhu, Niuping
Li, Junbao
Su, Chunlei
Jiang, Yibao
Yang, Yurong
author_sort Ren, Hongjie
collection PubMed
description Toxoplasmosis is one of the most common zoonotic diseases in the world. Felines excrete Toxoplasma gondii oocysts, which play a key role in the transmission of this protozoon. Pathological diagnoses were performed on four carcasses of captive tigers collected from 2019 to 2021 in China, and T. gondii was surveyed using serology, molecular analysis, and aetiology. Striated muscle samples of the tigers (n = 4) were bioassayed in mice. DNA derived from T. gondii tachyzoites was isolated and characterized using PCR–RFLP. The pathological diagnoses revealed that ageing, declined immune function, liver, and kidney failures caused the deaths in the tigers examined. A modified agglutination test (cut–off: 1:25) revealed that IgG antibodies to T. gondii were 100% (4/4) in the captive tigers. Two viable T. gondii strains (TgTigerCHn3 and TgTigerCHn4) were isolated from tiger striated muscles and seeded on the Vero cell culture for further propagation. The genotypes of TgTigerCHn3 and TgTigerCHn4 were ToxoDB#20 and ToxoDB#2, respectively. The two strains were avirulent for Swiss mice, which matched the ROP18 and ROP5 gene alleles of TgtigerCHn3 (3/4) and TgtigerCHn4 (3/3). Few brain tissue cysts (0–213) were observed in the mice after inoculation with TgTigerCHn3 and TgTigerCHn4. This is the first documented isolation of T. gondii ToxoDB#20 and ToxoDB#2 from tigers. The results provide additional direct evidence of tiger as intermediate hosts for T.gondii. Tigers in the zoos may potentially transmit T. gondii to other animals and humans.
format Online
Article
Text
id pubmed-9713289
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-97132892022-12-02 Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains Ren, Hongjie Yang, Liulu Zhu, Niuping Li, Junbao Su, Chunlei Jiang, Yibao Yang, Yurong Int J Parasitol Parasites Wildl Article Toxoplasmosis is one of the most common zoonotic diseases in the world. Felines excrete Toxoplasma gondii oocysts, which play a key role in the transmission of this protozoon. Pathological diagnoses were performed on four carcasses of captive tigers collected from 2019 to 2021 in China, and T. gondii was surveyed using serology, molecular analysis, and aetiology. Striated muscle samples of the tigers (n = 4) were bioassayed in mice. DNA derived from T. gondii tachyzoites was isolated and characterized using PCR–RFLP. The pathological diagnoses revealed that ageing, declined immune function, liver, and kidney failures caused the deaths in the tigers examined. A modified agglutination test (cut–off: 1:25) revealed that IgG antibodies to T. gondii were 100% (4/4) in the captive tigers. Two viable T. gondii strains (TgTigerCHn3 and TgTigerCHn4) were isolated from tiger striated muscles and seeded on the Vero cell culture for further propagation. The genotypes of TgTigerCHn3 and TgTigerCHn4 were ToxoDB#20 and ToxoDB#2, respectively. The two strains were avirulent for Swiss mice, which matched the ROP18 and ROP5 gene alleles of TgtigerCHn3 (3/4) and TgtigerCHn4 (3/3). Few brain tissue cysts (0–213) were observed in the mice after inoculation with TgTigerCHn3 and TgTigerCHn4. This is the first documented isolation of T. gondii ToxoDB#20 and ToxoDB#2 from tigers. The results provide additional direct evidence of tiger as intermediate hosts for T.gondii. Tigers in the zoos may potentially transmit T. gondii to other animals and humans. Elsevier 2022-11-25 /pmc/articles/PMC9713289/ /pubmed/36467851 http://dx.doi.org/10.1016/j.ijppaw.2022.11.009 Text en © 2022 Published by Elsevier Ltd on behalf of Australian Society for Parasitology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ren, Hongjie
Yang, Liulu
Zhu, Niuping
Li, Junbao
Su, Chunlei
Jiang, Yibao
Yang, Yurong
Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title_full Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title_fullStr Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title_full_unstemmed Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title_short Additional evidence of tigers (Panthera tigris altaica) as intermediate hosts for Toxoplasma gondii through the isolation of viable strains
title_sort additional evidence of tigers (panthera tigris altaica) as intermediate hosts for toxoplasma gondii through the isolation of viable strains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713289/
https://www.ncbi.nlm.nih.gov/pubmed/36467851
http://dx.doi.org/10.1016/j.ijppaw.2022.11.009
work_keys_str_mv AT renhongjie additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT yangliulu additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT zhuniuping additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT lijunbao additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT suchunlei additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT jiangyibao additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains
AT yangyurong additionalevidenceoftigerspantheratigrisaltaicaasintermediatehostsfortoxoplasmagondiithroughtheisolationofviablestrains