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Preservation of antigen-specific responses in cryopreserved CD4(+) and CD8(+) T cells expanded with IL-2 and IL-7

OBJECTIVES: We sought to develop medium throughput standard operating procedures for screening cryopreserved human peripheral blood mononuclear cells (PBMCs) for CD4(+) and CD8(+) T cell responses to potential autoantigens. METHODS: Dendritic cells were loaded with a peptide cocktail from ubiquitous...

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Detalles Bibliográficos
Autores principales: Clarkson, Benjamin DS., Johnson, Renee K., Bingel, Corinna, Lothaller, Caroline, Howe, Charles L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713293/
https://www.ncbi.nlm.nih.gov/pubmed/36467614
http://dx.doi.org/10.1016/j.jtauto.2022.100173