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Preservation of antigen-specific responses in cryopreserved CD4(+) and CD8(+) T cells expanded with IL-2 and IL-7
OBJECTIVES: We sought to develop medium throughput standard operating procedures for screening cryopreserved human peripheral blood mononuclear cells (PBMCs) for CD4(+) and CD8(+) T cell responses to potential autoantigens. METHODS: Dendritic cells were loaded with a peptide cocktail from ubiquitous...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713293/ https://www.ncbi.nlm.nih.gov/pubmed/36467614 http://dx.doi.org/10.1016/j.jtauto.2022.100173 |