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High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water

The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de Dôme (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large...

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Autores principales: Jarrige, Domitille, Haridas, Sajeet, Bleykasten-Grosshans, Claudine, Joly, Muriel, Nadalig, Thierry, Sancelme, Martine, Vuilleumier, Stéphane, Grigoriev, Igor V, Amato, Pierre, Bringel, Françoise
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713403/
https://www.ncbi.nlm.nih.gov/pubmed/36259934
http://dx.doi.org/10.1093/g3journal/jkac282
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author Jarrige, Domitille
Haridas, Sajeet
Bleykasten-Grosshans, Claudine
Joly, Muriel
Nadalig, Thierry
Sancelme, Martine
Vuilleumier, Stéphane
Grigoriev, Igor V
Amato, Pierre
Bringel, Françoise
author_facet Jarrige, Domitille
Haridas, Sajeet
Bleykasten-Grosshans, Claudine
Joly, Muriel
Nadalig, Thierry
Sancelme, Martine
Vuilleumier, Stéphane
Grigoriev, Igor V
Amato, Pierre
Bringel, Françoise
author_sort Jarrige, Domitille
collection PubMed
description The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de Dôme (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large-scale contigs ranging from 90 kb to 5.56 Mb, and another 27.2 kb contig representing the complete circular mitochondrial genome. In total, 8,234 proteins were predicted from the genome sequence. The mitochondrial genome shows 16.2% cgu codon usage for arginine but has no canonical cognate tRNA to translate this codon. Detected transposable element (TE)-related sequences account for about 0.63% of the assembled genome. A dataset of 2,068 hand-picked public environmental metagenomes, representing over 20 Tbp of raw reads, was probed for D. hungarica related ITS sequences, and revealed worldwide distribution of this species, particularly in aerial habitats. Growth experiments suggested a psychrophilic phenotype and the ability to disperse by producing ballistospores. The high-quality assembled genome obtained for this D. hungarica strain will help investigate the behavior and ecological functions of this species in the environment.
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spelling pubmed-97134032022-12-02 High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water Jarrige, Domitille Haridas, Sajeet Bleykasten-Grosshans, Claudine Joly, Muriel Nadalig, Thierry Sancelme, Martine Vuilleumier, Stéphane Grigoriev, Igor V Amato, Pierre Bringel, Françoise G3 (Bethesda) Investigation The genome of the basidiomycete yeast Dioszegia hungarica strain PDD-24b-2 isolated from cloud water at the summit of puy de Dôme (France) was sequenced using a hybrid PacBio and Illumina sequencing strategy. The obtained assembled genome of 20.98 Mb and a GC content of 57% is structured in 16 large-scale contigs ranging from 90 kb to 5.56 Mb, and another 27.2 kb contig representing the complete circular mitochondrial genome. In total, 8,234 proteins were predicted from the genome sequence. The mitochondrial genome shows 16.2% cgu codon usage for arginine but has no canonical cognate tRNA to translate this codon. Detected transposable element (TE)-related sequences account for about 0.63% of the assembled genome. A dataset of 2,068 hand-picked public environmental metagenomes, representing over 20 Tbp of raw reads, was probed for D. hungarica related ITS sequences, and revealed worldwide distribution of this species, particularly in aerial habitats. Growth experiments suggested a psychrophilic phenotype and the ability to disperse by producing ballistospores. The high-quality assembled genome obtained for this D. hungarica strain will help investigate the behavior and ecological functions of this species in the environment. Oxford University Press 2022-10-19 /pmc/articles/PMC9713403/ /pubmed/36259934 http://dx.doi.org/10.1093/g3journal/jkac282 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Jarrige, Domitille
Haridas, Sajeet
Bleykasten-Grosshans, Claudine
Joly, Muriel
Nadalig, Thierry
Sancelme, Martine
Vuilleumier, Stéphane
Grigoriev, Igor V
Amato, Pierre
Bringel, Françoise
High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title_full High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title_fullStr High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title_full_unstemmed High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title_short High-quality genome of the basidiomycete yeast Dioszegia hungarica PDD-24b-2 isolated from cloud water
title_sort high-quality genome of the basidiomycete yeast dioszegia hungarica pdd-24b-2 isolated from cloud water
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713403/
https://www.ncbi.nlm.nih.gov/pubmed/36259934
http://dx.doi.org/10.1093/g3journal/jkac282
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