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Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila

Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little is known about the immune response of gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp IgM and developed a model of c...

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Autores principales: Mu, Qingjiang, Dong, Zhaoran, Kong, Weiguang, Wang, Xinyou, Yu, Jiaqian, Ji, Wei, Su, Jianguo, Xu, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713697/
https://www.ncbi.nlm.nih.gov/pubmed/36466906
http://dx.doi.org/10.3389/fimmu.2022.1037517
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author Mu, Qingjiang
Dong, Zhaoran
Kong, Weiguang
Wang, Xinyou
Yu, Jiaqian
Ji, Wei
Su, Jianguo
Xu, Zhen
author_facet Mu, Qingjiang
Dong, Zhaoran
Kong, Weiguang
Wang, Xinyou
Yu, Jiaqian
Ji, Wei
Su, Jianguo
Xu, Zhen
author_sort Mu, Qingjiang
collection PubMed
description Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little is known about the immune response of gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp IgM and developed a model of carp infection with Aeromonas hydrophila via intraperitoneal injection. Our findings indicated that both innate and adaptive immune responses were effectively elicited after A. hydrophila infection. Upon bacterial infection, IgM(+) B cells were strongly induced in the gut and head kidney, and bacteria-specific IgM responses were detected in high levels both in the gut mucus and serum. Moreover, our results suggested that IgM responses may vary in different infection strategies. Overall, our findings revealed that the infected common carp exhibited high resistance to this representative enteropathogenic bacterium upon reinfection, suggesting that IgM plays a key role in the defense mechanisms of the gut against bacterial invasion. Significantly, the second injection of A. hydrophila induces strong local mucosal immunity in the gut, which is essential for protection against intestinal pathogens, providing reasonable insights for vaccine preparation.
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spelling pubmed-97136972022-12-02 Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila Mu, Qingjiang Dong, Zhaoran Kong, Weiguang Wang, Xinyou Yu, Jiaqian Ji, Wei Su, Jianguo Xu, Zhen Front Immunol Immunology Immunoglobulin (Ig) M is an important immune effector that protects organisms from a wide variety of pathogens. However, little is known about the immune response of gut mucosal IgM during bacterial invasion. Here, we generated polyclonal antibodies against common carp IgM and developed a model of carp infection with Aeromonas hydrophila via intraperitoneal injection. Our findings indicated that both innate and adaptive immune responses were effectively elicited after A. hydrophila infection. Upon bacterial infection, IgM(+) B cells were strongly induced in the gut and head kidney, and bacteria-specific IgM responses were detected in high levels both in the gut mucus and serum. Moreover, our results suggested that IgM responses may vary in different infection strategies. Overall, our findings revealed that the infected common carp exhibited high resistance to this representative enteropathogenic bacterium upon reinfection, suggesting that IgM plays a key role in the defense mechanisms of the gut against bacterial invasion. Significantly, the second injection of A. hydrophila induces strong local mucosal immunity in the gut, which is essential for protection against intestinal pathogens, providing reasonable insights for vaccine preparation. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9713697/ /pubmed/36466906 http://dx.doi.org/10.3389/fimmu.2022.1037517 Text en Copyright © 2022 Mu, Dong, Kong, Wang, Yu, Ji, Su and Xu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mu, Qingjiang
Dong, Zhaoran
Kong, Weiguang
Wang, Xinyou
Yu, Jiaqian
Ji, Wei
Su, Jianguo
Xu, Zhen
Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title_full Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title_fullStr Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title_full_unstemmed Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title_short Response of immunoglobulin M in gut mucosal immunity of common carp (Cyprinus carpio) infected with Aeromonas hydrophila
title_sort response of immunoglobulin m in gut mucosal immunity of common carp (cyprinus carpio) infected with aeromonas hydrophila
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9713697/
https://www.ncbi.nlm.nih.gov/pubmed/36466906
http://dx.doi.org/10.3389/fimmu.2022.1037517
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