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Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application

In recent years, STROBY (50% Kresoxim-methyl) has been widely used to control tobacco brown spot in Guizhou Province, China. As a broad-spectrum fungicide, STROBY targets not only phytopathogens, but also affects many other microorganisms including those pathogenic, beneficial, or neutral to the pla...

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Autores principales: Xiang, Li-Gang, Wang, Han-Cheng, Cai, Liu-Ti, Guo, Tao, Luo, Fei, Hsiang, Tom, Yu, Zhi-He
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714265/
https://www.ncbi.nlm.nih.gov/pubmed/36466663
http://dx.doi.org/10.3389/fmicb.2022.1068158
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author Xiang, Li-Gang
Wang, Han-Cheng
Cai, Liu-Ti
Guo, Tao
Luo, Fei
Hsiang, Tom
Yu, Zhi-He
author_facet Xiang, Li-Gang
Wang, Han-Cheng
Cai, Liu-Ti
Guo, Tao
Luo, Fei
Hsiang, Tom
Yu, Zhi-He
author_sort Xiang, Li-Gang
collection PubMed
description In recent years, STROBY (50% Kresoxim-methyl) has been widely used to control tobacco brown spot in Guizhou Province, China. As a broad-spectrum fungicide, STROBY targets not only phytopathogens, but also affects many other microorganisms including those pathogenic, beneficial, or neutral to the plant hosts. To understand the effects of STROBY on the phyllosphere microbial communities of tobacco leaves during the development of tobacco brown spot, the fungal and bacterial communities of symptomatic and asymptomatic leaves at four time points, before spraying (August 29) and after spraying (September 3, 8, and 13), were investigated using the Illumina high-throughput sequencing. The results showed that STROBY had significant effects on the phyllosphere microbial communities of tobacco leaves. Microbial communities in asymptomatic leaves were more greatly affected than their counterparts in symptomatic leaves, and fungal communities were more sensitive than bacterial communities. Throughout the experiment, the most common genera in symptomatic leaves were Alternaria, Pseudomonas, Pantoea, and Sphingomonas, and in asymptomatic leaves, these were Golubevia and Pantoea. After spraying, the alpha diversity of fungal communities increased in symptomatic leaves and decreased in asymptomatic leaves, while the alpha diversity of bacteria increased in both types of leaves. Beta diversity showed that in asymptomatic leaves, the fungal communities in the first stage was significantly different from the remaining three stages. In contrast, the fungal communities in symptomatic leaves and the bacterial communities in all leaves did not fluctuate significantly during the four stages. Before spraying (August 29), the dominant functions of the fungal community were animal pathogen, endophyte, plant pathogen, and wood saprotroph. Whereas after spraying (September 3, 8, and 13), the proportion of the above fungal functions decreased and the unassigned functions increased, especially in asymptomatic leaves. This study describes the effects of STROBY application and tobacco brown spot presence in shaping the leaf phyllosphere microbial communities, and provides insights into the microbial community effects on tobacco leaves of a strobilurin fungicide.
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spelling pubmed-97142652022-12-02 Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application Xiang, Li-Gang Wang, Han-Cheng Cai, Liu-Ti Guo, Tao Luo, Fei Hsiang, Tom Yu, Zhi-He Front Microbiol Microbiology In recent years, STROBY (50% Kresoxim-methyl) has been widely used to control tobacco brown spot in Guizhou Province, China. As a broad-spectrum fungicide, STROBY targets not only phytopathogens, but also affects many other microorganisms including those pathogenic, beneficial, or neutral to the plant hosts. To understand the effects of STROBY on the phyllosphere microbial communities of tobacco leaves during the development of tobacco brown spot, the fungal and bacterial communities of symptomatic and asymptomatic leaves at four time points, before spraying (August 29) and after spraying (September 3, 8, and 13), were investigated using the Illumina high-throughput sequencing. The results showed that STROBY had significant effects on the phyllosphere microbial communities of tobacco leaves. Microbial communities in asymptomatic leaves were more greatly affected than their counterparts in symptomatic leaves, and fungal communities were more sensitive than bacterial communities. Throughout the experiment, the most common genera in symptomatic leaves were Alternaria, Pseudomonas, Pantoea, and Sphingomonas, and in asymptomatic leaves, these were Golubevia and Pantoea. After spraying, the alpha diversity of fungal communities increased in symptomatic leaves and decreased in asymptomatic leaves, while the alpha diversity of bacteria increased in both types of leaves. Beta diversity showed that in asymptomatic leaves, the fungal communities in the first stage was significantly different from the remaining three stages. In contrast, the fungal communities in symptomatic leaves and the bacterial communities in all leaves did not fluctuate significantly during the four stages. Before spraying (August 29), the dominant functions of the fungal community were animal pathogen, endophyte, plant pathogen, and wood saprotroph. Whereas after spraying (September 3, 8, and 13), the proportion of the above fungal functions decreased and the unassigned functions increased, especially in asymptomatic leaves. This study describes the effects of STROBY application and tobacco brown spot presence in shaping the leaf phyllosphere microbial communities, and provides insights into the microbial community effects on tobacco leaves of a strobilurin fungicide. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9714265/ /pubmed/36466663 http://dx.doi.org/10.3389/fmicb.2022.1068158 Text en Copyright © 2022 Xiang, Wang, Cai, Guo, Luo, Hsiang and Yu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xiang, Li-Gang
Wang, Han-Cheng
Cai, Liu-Ti
Guo, Tao
Luo, Fei
Hsiang, Tom
Yu, Zhi-He
Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title_full Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title_fullStr Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title_full_unstemmed Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title_short Variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
title_sort variations in leaf phyllosphere microbial communities and development of tobacco brown spot before and after fungicide application
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714265/
https://www.ncbi.nlm.nih.gov/pubmed/36466663
http://dx.doi.org/10.3389/fmicb.2022.1068158
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