Cargando…
Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
Regeneration is extremely important to pepper genetic development; however, the molecular mechanisms of how the callus reactivates cell proliferation and promotes cell reprogramming remain elusive in pepper. In the present study, C. baccatum (HNUCB81 and HNUCB226) and C. chinense (HNUCC22 and HNUCC1...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714296/ https://www.ncbi.nlm.nih.gov/pubmed/36466290 http://dx.doi.org/10.3389/fpls.2022.1025497 |
_version_ | 1784842192223731712 |
---|---|
author | Shu, Huangying Zhang, Yu He, Chengyao Altaf, Muhammad Ahsan Hao, Yuanyuan Liao, Daolong Li, Lin Li, Caichao Fu, Huizhen Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei |
author_facet | Shu, Huangying Zhang, Yu He, Chengyao Altaf, Muhammad Ahsan Hao, Yuanyuan Liao, Daolong Li, Lin Li, Caichao Fu, Huizhen Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei |
author_sort | Shu, Huangying |
collection | PubMed |
description | Regeneration is extremely important to pepper genetic development; however, the molecular mechanisms of how the callus reactivates cell proliferation and promotes cell reprogramming remain elusive in pepper. In the present study, C. baccatum (HNUCB81 and HNUCB226) and C. chinense (HNUCC22 and HNUCC16) were analyzed to reveal callus initiation by in vitro regeneration, histology, and transcriptome. We successfully established an efficient in vitro regeneration system of two cultivars to monitor the callus induction of differential genotypes, and the regenerated plants were obtained. Compared to C. chinense, there was a higher callus induction rate in C. baccatum. The phenotype of C. baccatum changed significantly and formed vascular tissue faster than C. chinense. The KEGG enrichment analysis found that plant hormone transduction and starch and sucrose metabolism pathways were significantly enriched. In addition, we identified that the WOX7 gene was significantly up-regulated in HNUCB81 and HNUCB226 than that in HNUCC22 and HNUCC16, which may be a potential function in callus formation. These results provided a promising strategy to improve the regeneration and transformation of pepper plants. |
format | Online Article Text |
id | pubmed-9714296 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97142962022-12-02 Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum Shu, Huangying Zhang, Yu He, Chengyao Altaf, Muhammad Ahsan Hao, Yuanyuan Liao, Daolong Li, Lin Li, Caichao Fu, Huizhen Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei Front Plant Sci Plant Science Regeneration is extremely important to pepper genetic development; however, the molecular mechanisms of how the callus reactivates cell proliferation and promotes cell reprogramming remain elusive in pepper. In the present study, C. baccatum (HNUCB81 and HNUCB226) and C. chinense (HNUCC22 and HNUCC16) were analyzed to reveal callus initiation by in vitro regeneration, histology, and transcriptome. We successfully established an efficient in vitro regeneration system of two cultivars to monitor the callus induction of differential genotypes, and the regenerated plants were obtained. Compared to C. chinense, there was a higher callus induction rate in C. baccatum. The phenotype of C. baccatum changed significantly and formed vascular tissue faster than C. chinense. The KEGG enrichment analysis found that plant hormone transduction and starch and sucrose metabolism pathways were significantly enriched. In addition, we identified that the WOX7 gene was significantly up-regulated in HNUCB81 and HNUCB226 than that in HNUCC22 and HNUCC16, which may be a potential function in callus formation. These results provided a promising strategy to improve the regeneration and transformation of pepper plants. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9714296/ /pubmed/36466290 http://dx.doi.org/10.3389/fpls.2022.1025497 Text en Copyright © 2022 Shu, Zhang, He, Altaf, Hao, Liao, Li, Li, Fu, Cheng, Zhu and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Shu, Huangying Zhang, Yu He, Chengyao Altaf, Muhammad Ahsan Hao, Yuanyuan Liao, Daolong Li, Lin Li, Caichao Fu, Huizhen Cheng, Shanhan Zhu, Guopeng Wang, Zhiwei Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum |
title | Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
|
title_full | Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
|
title_fullStr | Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
|
title_full_unstemmed | Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
|
title_short | Establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in Capsicum chinense and Capsicum baccatum
|
title_sort | establishment of in vitro regeneration system and molecular analysis of early development of somatic callus in capsicum chinense and capsicum baccatum |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714296/ https://www.ncbi.nlm.nih.gov/pubmed/36466290 http://dx.doi.org/10.3389/fpls.2022.1025497 |
work_keys_str_mv | AT shuhuangying establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT zhangyu establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT hechengyao establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT altafmuhammadahsan establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT haoyuanyuan establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT liaodaolong establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT lilin establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT licaichao establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT fuhuizhen establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT chengshanhan establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT zhuguopeng establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum AT wangzhiwei establishmentofinvitroregenerationsystemandmolecularanalysisofearlydevelopmentofsomaticcallusincapsicumchinenseandcapsicumbaccatum |