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Energy metabolism in skeletal muscle cells from donors with different body mass index

Obesity and physical inactivity have a profound impact on skeletal muscle metabolism. In the present work, we have investigated differences in protein expression and energy metabolism in primary human skeletal muscle cells established from lean donors (BMI<25 kg/m(2)) and individuals with obesity...

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Autores principales: Katare, Parmeshwar B., Dalmao-Fernandez, Andrea, Mengeste, Abel M., Hamarsland, Håvard, Ellefsen, Stian, Bakke, Hege G., Kase, Eili Tranheim, Thoresen, G. Hege, Rustan, Arild C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714574/
https://www.ncbi.nlm.nih.gov/pubmed/36467688
http://dx.doi.org/10.3389/fphys.2022.982842
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author Katare, Parmeshwar B.
Dalmao-Fernandez, Andrea
Mengeste, Abel M.
Hamarsland, Håvard
Ellefsen, Stian
Bakke, Hege G.
Kase, Eili Tranheim
Thoresen, G. Hege
Rustan, Arild C.
author_facet Katare, Parmeshwar B.
Dalmao-Fernandez, Andrea
Mengeste, Abel M.
Hamarsland, Håvard
Ellefsen, Stian
Bakke, Hege G.
Kase, Eili Tranheim
Thoresen, G. Hege
Rustan, Arild C.
author_sort Katare, Parmeshwar B.
collection PubMed
description Obesity and physical inactivity have a profound impact on skeletal muscle metabolism. In the present work, we have investigated differences in protein expression and energy metabolism in primary human skeletal muscle cells established from lean donors (BMI<25 kg/m(2)) and individuals with obesity (BMI>30 kg/m(2)). Furthermore, we have studied the effect of fatty acid pretreatment on energy metabolism in myotubes from these donor groups. Alterations in protein expression were investigated using proteomic analysis, and energy metabolism was studied using radiolabeled substrates. Gene Ontology enrichment analysis showed that glycolytic, apoptotic, and hypoxia pathways were upregulated, whereas the pentose phosphate pathway was downregulated in myotubes from donors with obesity compared to myotubes from lean donors. Moreover, fatty acid, glucose, and amino acid uptake were increased in myotubes from individuals with obesity. However, fatty acid oxidation was reduced, glucose oxidation was increased in myotubes from subjects with obesity compared to cells from lean. Pretreatment of myotubes with palmitic acid (PA) or eicosapentaenoic acid (EPA) for 24 h increased glucose oxidation and oleic acid uptake. EPA pretreatment increased the glucose and fatty acid uptake and reduced leucine fractional oxidation in myotubes from donors with obesity. In conclusion, these results suggest that myotubes from individuals with obesity showed increased fatty acid, glucose, and amino acid uptake compared to cells from lean donors. Furthermore, myotubes from individuals with obesity had reduced fatty acid oxidative capacity, increased glucose oxidation, and a higher glycolytic reserve capacity compared to cells from lean donors. Fatty acid pretreatment enhances glucose metabolism, and EPA reduces oleic acid and leucine fractional oxidation in myotubes from donor with obesity, suggesting increased metabolic flexibility after EPA treatment.
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spelling pubmed-97145742022-12-02 Energy metabolism in skeletal muscle cells from donors with different body mass index Katare, Parmeshwar B. Dalmao-Fernandez, Andrea Mengeste, Abel M. Hamarsland, Håvard Ellefsen, Stian Bakke, Hege G. Kase, Eili Tranheim Thoresen, G. Hege Rustan, Arild C. Front Physiol Physiology Obesity and physical inactivity have a profound impact on skeletal muscle metabolism. In the present work, we have investigated differences in protein expression and energy metabolism in primary human skeletal muscle cells established from lean donors (BMI<25 kg/m(2)) and individuals with obesity (BMI>30 kg/m(2)). Furthermore, we have studied the effect of fatty acid pretreatment on energy metabolism in myotubes from these donor groups. Alterations in protein expression were investigated using proteomic analysis, and energy metabolism was studied using radiolabeled substrates. Gene Ontology enrichment analysis showed that glycolytic, apoptotic, and hypoxia pathways were upregulated, whereas the pentose phosphate pathway was downregulated in myotubes from donors with obesity compared to myotubes from lean donors. Moreover, fatty acid, glucose, and amino acid uptake were increased in myotubes from individuals with obesity. However, fatty acid oxidation was reduced, glucose oxidation was increased in myotubes from subjects with obesity compared to cells from lean. Pretreatment of myotubes with palmitic acid (PA) or eicosapentaenoic acid (EPA) for 24 h increased glucose oxidation and oleic acid uptake. EPA pretreatment increased the glucose and fatty acid uptake and reduced leucine fractional oxidation in myotubes from donors with obesity. In conclusion, these results suggest that myotubes from individuals with obesity showed increased fatty acid, glucose, and amino acid uptake compared to cells from lean donors. Furthermore, myotubes from individuals with obesity had reduced fatty acid oxidative capacity, increased glucose oxidation, and a higher glycolytic reserve capacity compared to cells from lean donors. Fatty acid pretreatment enhances glucose metabolism, and EPA reduces oleic acid and leucine fractional oxidation in myotubes from donor with obesity, suggesting increased metabolic flexibility after EPA treatment. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9714574/ /pubmed/36467688 http://dx.doi.org/10.3389/fphys.2022.982842 Text en Copyright © 2022 Katare, Dalmao-Fernandez, Mengeste, Hamarsland, Ellefsen, Bakke, Kase, Thoresen and Rustan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Katare, Parmeshwar B.
Dalmao-Fernandez, Andrea
Mengeste, Abel M.
Hamarsland, Håvard
Ellefsen, Stian
Bakke, Hege G.
Kase, Eili Tranheim
Thoresen, G. Hege
Rustan, Arild C.
Energy metabolism in skeletal muscle cells from donors with different body mass index
title Energy metabolism in skeletal muscle cells from donors with different body mass index
title_full Energy metabolism in skeletal muscle cells from donors with different body mass index
title_fullStr Energy metabolism in skeletal muscle cells from donors with different body mass index
title_full_unstemmed Energy metabolism in skeletal muscle cells from donors with different body mass index
title_short Energy metabolism in skeletal muscle cells from donors with different body mass index
title_sort energy metabolism in skeletal muscle cells from donors with different body mass index
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714574/
https://www.ncbi.nlm.nih.gov/pubmed/36467688
http://dx.doi.org/10.3389/fphys.2022.982842
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