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Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus

Bursaphelenchus xylophilu is a worldwide quarantine nematode, causing huge economic losses and ecological disasters in many countries. The sex ratio of B. xylophilus plays an important role in the nematode infestation. The laf-1-related genes are highly conserved in animals, playing crucial roles in...

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Autores principales: Sun, Shimiao, Wang, Jinghan, Liu, Wenyi, Chen, Jing, Zhou, Lifeng, Wu, Choufei, Yu, Hongshi, Hu, Jiafu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714631/
https://www.ncbi.nlm.nih.gov/pubmed/36467707
http://dx.doi.org/10.3389/fphys.2022.1024409
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author Sun, Shimiao
Wang, Jinghan
Liu, Wenyi
Chen, Jing
Zhou, Lifeng
Wu, Choufei
Yu, Hongshi
Hu, Jiafu
author_facet Sun, Shimiao
Wang, Jinghan
Liu, Wenyi
Chen, Jing
Zhou, Lifeng
Wu, Choufei
Yu, Hongshi
Hu, Jiafu
author_sort Sun, Shimiao
collection PubMed
description Bursaphelenchus xylophilu is a worldwide quarantine nematode, causing huge economic losses and ecological disasters in many countries. The sex ratio of B. xylophilus plays an important role in the nematode infestation. The laf-1-related genes are highly conserved in animals, playing crucial roles in sex determination. Therefore, we investigated the expression pattern and biological function of its orthologue, Bxy-laf-1 in B. xylophilus. Bxy-laf-1 has two typical conserved DNA-binding domains, DEAD and Helicase C. The real-time quantitative PCR data revealed that Bxy-laf-1 expression was required throughout the entire life of B. xylophilus, with the maximum expression in the J2 stage and the lowest expression in the adult stage. mRNA in situ hybridization showed that Bxy-laf-1 is mainly located in the cephalopharynx and reproductive organs of B. xylophilus. RNA interference (RNAi) indicated that the head swing frequency was dramatically decreased. The RNA interference results displayed that a significant reduction in motility was observed in the hatched larvae. The female to male sex ratio was also decreased in the F0 and F1 generations, but recovered in the F2 generation. The tail of female adults with eggs in the belly appeared deformities. This phenomenon appeared in the F0 and F1 generations, but recovered in the F2 generation. Bxy-laf-1 is a typical sex-determination gene with distinct expression patterns in males and females. As demonstrated in other species, the sex ratio was altered after knocking down Bxy-laf-1 expression. The results of this study contribute to our understanding of the molecular processes of Bxy-laf-1 in B. xylophilus, which may provide clues about how to control pine wilt disease by inhibiting ontogenic growth and reducing nematode fertility.
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spelling pubmed-97146312022-12-02 Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus Sun, Shimiao Wang, Jinghan Liu, Wenyi Chen, Jing Zhou, Lifeng Wu, Choufei Yu, Hongshi Hu, Jiafu Front Physiol Physiology Bursaphelenchus xylophilu is a worldwide quarantine nematode, causing huge economic losses and ecological disasters in many countries. The sex ratio of B. xylophilus plays an important role in the nematode infestation. The laf-1-related genes are highly conserved in animals, playing crucial roles in sex determination. Therefore, we investigated the expression pattern and biological function of its orthologue, Bxy-laf-1 in B. xylophilus. Bxy-laf-1 has two typical conserved DNA-binding domains, DEAD and Helicase C. The real-time quantitative PCR data revealed that Bxy-laf-1 expression was required throughout the entire life of B. xylophilus, with the maximum expression in the J2 stage and the lowest expression in the adult stage. mRNA in situ hybridization showed that Bxy-laf-1 is mainly located in the cephalopharynx and reproductive organs of B. xylophilus. RNA interference (RNAi) indicated that the head swing frequency was dramatically decreased. The RNA interference results displayed that a significant reduction in motility was observed in the hatched larvae. The female to male sex ratio was also decreased in the F0 and F1 generations, but recovered in the F2 generation. The tail of female adults with eggs in the belly appeared deformities. This phenomenon appeared in the F0 and F1 generations, but recovered in the F2 generation. Bxy-laf-1 is a typical sex-determination gene with distinct expression patterns in males and females. As demonstrated in other species, the sex ratio was altered after knocking down Bxy-laf-1 expression. The results of this study contribute to our understanding of the molecular processes of Bxy-laf-1 in B. xylophilus, which may provide clues about how to control pine wilt disease by inhibiting ontogenic growth and reducing nematode fertility. Frontiers Media S.A. 2022-11-17 /pmc/articles/PMC9714631/ /pubmed/36467707 http://dx.doi.org/10.3389/fphys.2022.1024409 Text en Copyright © 2022 Sun, Wang, Liu, Chen, Zhou, Wu, Yu and Hu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Sun, Shimiao
Wang, Jinghan
Liu, Wenyi
Chen, Jing
Zhou, Lifeng
Wu, Choufei
Yu, Hongshi
Hu, Jiafu
Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title_full Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title_fullStr Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title_full_unstemmed Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title_short Regulatory roles of Bxy-laf-1 in reproductive behaviour of Bursaphelenchus xylophilus
title_sort regulatory roles of bxy-laf-1 in reproductive behaviour of bursaphelenchus xylophilus
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714631/
https://www.ncbi.nlm.nih.gov/pubmed/36467707
http://dx.doi.org/10.3389/fphys.2022.1024409
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