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Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle

In recent years, many new immunologically active peptides from insects have been identified. Unfortunately, in most cases, their physiological functions are not fully known. One example is yamamarin, a pentapeptide isolated from the caterpillars of the Antheraea yamamai moth. This peptide has strong...

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Autores principales: Walkowiak-Nowicka, Karolina, Chowański, Szymon, Pacholska-Bogalska, Joanna, Adamski, Zbigniew, Kuczer, Mariola, Rosiński, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714928/
https://www.ncbi.nlm.nih.gov/pubmed/36454989
http://dx.doi.org/10.1371/journal.pone.0278473
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author Walkowiak-Nowicka, Karolina
Chowański, Szymon
Pacholska-Bogalska, Joanna
Adamski, Zbigniew
Kuczer, Mariola
Rosiński, Grzegorz
author_facet Walkowiak-Nowicka, Karolina
Chowański, Szymon
Pacholska-Bogalska, Joanna
Adamski, Zbigniew
Kuczer, Mariola
Rosiński, Grzegorz
author_sort Walkowiak-Nowicka, Karolina
collection PubMed
description In recent years, many new immunologically active peptides from insects have been identified. Unfortunately, in most cases, their physiological functions are not fully known. One example is yamamarin, a pentapeptide isolated from the caterpillars of the Antheraea yamamai moth. This peptide has strong antiproliferative properties and is probably involved in the regulation of diapause. Additionally, antiviral activity was discovered. The results of the research presented in this paper are, to our knowledge, the first attempt to characterize the biological effects of yamamarin on the functioning of the reproductive processes and embryonic development of insects using a model species, the beetle Tenebrio molitor, a commonly known pest of grain storage. Simultaneously, we tested the possible activity of the molecule in an in vivo system. In this research, we present the multifaceted effects of yamamarin in this beetle. We show that yamamarin influences ovarian growth and development, maturation of terminal oocytes, level of vitellogenin gene transcript, the number of laid eggs, duration of embryonic development, and larval hatching. In experiments with palmitic acid-conjugated yamamarin (C16-yamamarin), we also showed that this peptide is a useful starting molecule for the synthesis of biopharmaceuticals or new peptidomimetics with gonadotropic activity and effects on embryonic development. The data obtained additionally provide new knowledge about the possible function of yamamarin in insect physiology, pointing to the important role of this pentapeptide as a regulator of reproductive processes and embryonic development in a heterologous bioassay with T. molitor.
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spelling pubmed-97149282022-12-02 Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle Walkowiak-Nowicka, Karolina Chowański, Szymon Pacholska-Bogalska, Joanna Adamski, Zbigniew Kuczer, Mariola Rosiński, Grzegorz PLoS One Research Article In recent years, many new immunologically active peptides from insects have been identified. Unfortunately, in most cases, their physiological functions are not fully known. One example is yamamarin, a pentapeptide isolated from the caterpillars of the Antheraea yamamai moth. This peptide has strong antiproliferative properties and is probably involved in the regulation of diapause. Additionally, antiviral activity was discovered. The results of the research presented in this paper are, to our knowledge, the first attempt to characterize the biological effects of yamamarin on the functioning of the reproductive processes and embryonic development of insects using a model species, the beetle Tenebrio molitor, a commonly known pest of grain storage. Simultaneously, we tested the possible activity of the molecule in an in vivo system. In this research, we present the multifaceted effects of yamamarin in this beetle. We show that yamamarin influences ovarian growth and development, maturation of terminal oocytes, level of vitellogenin gene transcript, the number of laid eggs, duration of embryonic development, and larval hatching. In experiments with palmitic acid-conjugated yamamarin (C16-yamamarin), we also showed that this peptide is a useful starting molecule for the synthesis of biopharmaceuticals or new peptidomimetics with gonadotropic activity and effects on embryonic development. The data obtained additionally provide new knowledge about the possible function of yamamarin in insect physiology, pointing to the important role of this pentapeptide as a regulator of reproductive processes and embryonic development in a heterologous bioassay with T. molitor. Public Library of Science 2022-12-01 /pmc/articles/PMC9714928/ /pubmed/36454989 http://dx.doi.org/10.1371/journal.pone.0278473 Text en © 2022 Walkowiak-Nowicka et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Walkowiak-Nowicka, Karolina
Chowański, Szymon
Pacholska-Bogalska, Joanna
Adamski, Zbigniew
Kuczer, Mariola
Rosiński, Grzegorz
Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title_full Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title_fullStr Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title_full_unstemmed Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title_short Antheraea peptide and its analog: Their influence on the maturation of the reproductive system, embryogenesis, and early larval development in Tenebrio molitor L. beetle
title_sort antheraea peptide and its analog: their influence on the maturation of the reproductive system, embryogenesis, and early larval development in tenebrio molitor l. beetle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714928/
https://www.ncbi.nlm.nih.gov/pubmed/36454989
http://dx.doi.org/10.1371/journal.pone.0278473
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