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DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis

The early-stage pathologies of frontotemporal lobal degeneration (FTLD) remain largely unknown. In VCP(T262A)-KI mice carrying VCP gene mutation linked to FTLD, insufficient DNA damage repair in neural stem/progenitor cells (NSCs) activated DNA-PK and CDK1 that disabled MCM3 essential for the G1/S c...

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Autores principales: Homma, Hidenori, Tanaka, Hikari, Jin, Meihua, Jin, Xiaocen, Huang, Yong, Yoshioka, Yuki, Bertens, Christian JF, Tsumaki, Kohei, Kondo, Kanoh, Shiwaku, Hiroki, Tagawa, Kazuhiko, Akatsu, Hiroyasu, Atsuta, Naoki, Katsuno, Masahisa, Furukawa, Katsutoshi, Ishiki, Aiko, Waragai, Masaaki, Ohtomo, Gaku, Iwata, Atsushi, Yokota, Takanori, Inoue, Haruhisa, Arai, Hiroyuki, Sobue, Gen, Sone, Masaki, Fujita, Kyota, Okazawa, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715434/
https://www.ncbi.nlm.nih.gov/pubmed/34130995
http://dx.doi.org/10.26508/lsa.202101022
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author Homma, Hidenori
Tanaka, Hikari
Jin, Meihua
Jin, Xiaocen
Huang, Yong
Yoshioka, Yuki
Bertens, Christian JF
Tsumaki, Kohei
Kondo, Kanoh
Shiwaku, Hiroki
Tagawa, Kazuhiko
Akatsu, Hiroyasu
Atsuta, Naoki
Katsuno, Masahisa
Furukawa, Katsutoshi
Ishiki, Aiko
Waragai, Masaaki
Ohtomo, Gaku
Iwata, Atsushi
Yokota, Takanori
Inoue, Haruhisa
Arai, Hiroyuki
Sobue, Gen
Sone, Masaki
Fujita, Kyota
Okazawa, Hitoshi
author_facet Homma, Hidenori
Tanaka, Hikari
Jin, Meihua
Jin, Xiaocen
Huang, Yong
Yoshioka, Yuki
Bertens, Christian JF
Tsumaki, Kohei
Kondo, Kanoh
Shiwaku, Hiroki
Tagawa, Kazuhiko
Akatsu, Hiroyasu
Atsuta, Naoki
Katsuno, Masahisa
Furukawa, Katsutoshi
Ishiki, Aiko
Waragai, Masaaki
Ohtomo, Gaku
Iwata, Atsushi
Yokota, Takanori
Inoue, Haruhisa
Arai, Hiroyuki
Sobue, Gen
Sone, Masaki
Fujita, Kyota
Okazawa, Hitoshi
author_sort Homma, Hidenori
collection PubMed
description The early-stage pathologies of frontotemporal lobal degeneration (FTLD) remain largely unknown. In VCP(T262A)-KI mice carrying VCP gene mutation linked to FTLD, insufficient DNA damage repair in neural stem/progenitor cells (NSCs) activated DNA-PK and CDK1 that disabled MCM3 essential for the G1/S cell cycle transition. Abnormal neural exit produced neurons carrying over unrepaired DNA damage and induced early-stage transcriptional repression-induced atypical cell death (TRIAD) necrosis accompanied by the specific markers pSer46-MARCKS and YAP. In utero gene therapy expressing normal VCP or non-phosphorylated mutant MCM3 rescued DNA damage, neuronal necrosis, cognitive function, and TDP43 aggregation in adult neurons of VCP(T262A)-KI mice, whereas similar therapy in adulthood was less effective. The similar early-stage neuronal necrosis was detected in PGRN(R504X)-KI, CHMP2B(Q165X)-KI, and TDP(N267S)-KI mice, and blocked by embryonic treatment with AAV–non-phospho-MCM3. Moreover, YAP-dependent necrosis occurred in neurons of human FTLD patients, and consistently pSer46-MARCKS was increased in cerebrospinal fluid (CSF) and serum of these patients. Collectively, developmental stress followed by early-stage neuronal necrosis is a potential target for therapeutics and one of the earliest general biomarkers for FTLD.
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spelling pubmed-97154342022-12-03 DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis Homma, Hidenori Tanaka, Hikari Jin, Meihua Jin, Xiaocen Huang, Yong Yoshioka, Yuki Bertens, Christian JF Tsumaki, Kohei Kondo, Kanoh Shiwaku, Hiroki Tagawa, Kazuhiko Akatsu, Hiroyasu Atsuta, Naoki Katsuno, Masahisa Furukawa, Katsutoshi Ishiki, Aiko Waragai, Masaaki Ohtomo, Gaku Iwata, Atsushi Yokota, Takanori Inoue, Haruhisa Arai, Hiroyuki Sobue, Gen Sone, Masaki Fujita, Kyota Okazawa, Hitoshi Life Sci Alliance Research Articles The early-stage pathologies of frontotemporal lobal degeneration (FTLD) remain largely unknown. In VCP(T262A)-KI mice carrying VCP gene mutation linked to FTLD, insufficient DNA damage repair in neural stem/progenitor cells (NSCs) activated DNA-PK and CDK1 that disabled MCM3 essential for the G1/S cell cycle transition. Abnormal neural exit produced neurons carrying over unrepaired DNA damage and induced early-stage transcriptional repression-induced atypical cell death (TRIAD) necrosis accompanied by the specific markers pSer46-MARCKS and YAP. In utero gene therapy expressing normal VCP or non-phosphorylated mutant MCM3 rescued DNA damage, neuronal necrosis, cognitive function, and TDP43 aggregation in adult neurons of VCP(T262A)-KI mice, whereas similar therapy in adulthood was less effective. The similar early-stage neuronal necrosis was detected in PGRN(R504X)-KI, CHMP2B(Q165X)-KI, and TDP(N267S)-KI mice, and blocked by embryonic treatment with AAV–non-phospho-MCM3. Moreover, YAP-dependent necrosis occurred in neurons of human FTLD patients, and consistently pSer46-MARCKS was increased in cerebrospinal fluid (CSF) and serum of these patients. Collectively, developmental stress followed by early-stage neuronal necrosis is a potential target for therapeutics and one of the earliest general biomarkers for FTLD. Life Science Alliance LLC 2021-06-15 /pmc/articles/PMC9715434/ /pubmed/34130995 http://dx.doi.org/10.26508/lsa.202101022 Text en © 2021 Homma et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Homma, Hidenori
Tanaka, Hikari
Jin, Meihua
Jin, Xiaocen
Huang, Yong
Yoshioka, Yuki
Bertens, Christian JF
Tsumaki, Kohei
Kondo, Kanoh
Shiwaku, Hiroki
Tagawa, Kazuhiko
Akatsu, Hiroyasu
Atsuta, Naoki
Katsuno, Masahisa
Furukawa, Katsutoshi
Ishiki, Aiko
Waragai, Masaaki
Ohtomo, Gaku
Iwata, Atsushi
Yokota, Takanori
Inoue, Haruhisa
Arai, Hiroyuki
Sobue, Gen
Sone, Masaki
Fujita, Kyota
Okazawa, Hitoshi
DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title_full DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title_fullStr DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title_full_unstemmed DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title_short DNA damage in embryonic neural stem cell determines FTLDs’ fate via early-stage neuronal necrosis
title_sort dna damage in embryonic neural stem cell determines ftlds’ fate via early-stage neuronal necrosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715434/
https://www.ncbi.nlm.nih.gov/pubmed/34130995
http://dx.doi.org/10.26508/lsa.202101022
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