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Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago
PURPOSE: Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens. METHODS: We assessed the analysis of ten...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715498/ https://www.ncbi.nlm.nih.gov/pubmed/36454408 http://dx.doi.org/10.1007/s11419-022-00614-0 |
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author | Di Candia, Domenico Boracchi, Michele Gentile, Guendalina Giordano, Gaia Zoja, Riccardo |
author_facet | Di Candia, Domenico Boracchi, Michele Gentile, Guendalina Giordano, Gaia Zoja, Riccardo |
author_sort | Di Candia, Domenico |
collection | PubMed |
description | PURPOSE: Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens. METHODS: We assessed the analysis of ten fresh liver samples collected from heroin-related deaths and then histologically processed the same samples. The embedded blocks were then extracted by means of a new extracting method and the eluates were measured. We also selected five toxicological cases of heroin-related fatalities that were examined 20 years ago, collected the toxicological result documents of the analysis that were carried out at the time and then processed the corresponding FFPE liver samples that were stored in the archives. RESULTS: We managed to isolate heroine-related metabolites from 20-year-old paraffin-embedded blocks and calculated ratios to evaluate the performance of our new extraction. CONCLUSIONS: According to our study, it is feasible to carry out a toxicological examination on old histological samples and, therefore, this matrix can be considered as a new alternative specimen for chemical-analytical evaluations of past cases or when fresh samples are not available anymore. The new extractive method was evaluated as efficient in treating these complex, paraffin-embedded samples. It was surprising that the target compounds could be quantitated from FFPE bocks created as long as 20 years ago. |
format | Online Article Text |
id | pubmed-9715498 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-97154982022-12-03 Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago Di Candia, Domenico Boracchi, Michele Gentile, Guendalina Giordano, Gaia Zoja, Riccardo Forensic Toxicol Original Article PURPOSE: Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens. METHODS: We assessed the analysis of ten fresh liver samples collected from heroin-related deaths and then histologically processed the same samples. The embedded blocks were then extracted by means of a new extracting method and the eluates were measured. We also selected five toxicological cases of heroin-related fatalities that were examined 20 years ago, collected the toxicological result documents of the analysis that were carried out at the time and then processed the corresponding FFPE liver samples that were stored in the archives. RESULTS: We managed to isolate heroine-related metabolites from 20-year-old paraffin-embedded blocks and calculated ratios to evaluate the performance of our new extraction. CONCLUSIONS: According to our study, it is feasible to carry out a toxicological examination on old histological samples and, therefore, this matrix can be considered as a new alternative specimen for chemical-analytical evaluations of past cases or when fresh samples are not available anymore. The new extractive method was evaluated as efficient in treating these complex, paraffin-embedded samples. It was surprising that the target compounds could be quantitated from FFPE bocks created as long as 20 years ago. Springer Nature Singapore 2022-03-14 2022 /pmc/articles/PMC9715498/ /pubmed/36454408 http://dx.doi.org/10.1007/s11419-022-00614-0 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Di Candia, Domenico Boracchi, Michele Gentile, Guendalina Giordano, Gaia Zoja, Riccardo Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title | Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title_full | Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title_fullStr | Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title_full_unstemmed | Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title_short | Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
title_sort | histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715498/ https://www.ncbi.nlm.nih.gov/pubmed/36454408 http://dx.doi.org/10.1007/s11419-022-00614-0 |
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