Cargando…

SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans

Potato is the most important non-grain food in the world, while late blight caused by Phytophthora infestans seriously threatens the production of potato. Since pathogen-associated molecular patterns (PAMPs) are relatively conserved, PAMP-triggered immunity (PTI) can provide durable resistance to la...

Descripción completa

Detalles Bibliográficos
Autores principales: Mu, Yang, Guo, Xiao, Yu, Jian, Wang, Ruxun, Liu, Zeng, Hu, Kefan, Song, Jingyi, Chen, Lin, Song, Botao, Du, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715588/
https://www.ncbi.nlm.nih.gov/pubmed/36466288
http://dx.doi.org/10.3389/fpls.2022.1036637
_version_ 1784842483935477760
author Mu, Yang
Guo, Xiao
Yu, Jian
Wang, Ruxun
Liu, Zeng
Hu, Kefan
Song, Jingyi
Chen, Lin
Song, Botao
Du, Juan
author_facet Mu, Yang
Guo, Xiao
Yu, Jian
Wang, Ruxun
Liu, Zeng
Hu, Kefan
Song, Jingyi
Chen, Lin
Song, Botao
Du, Juan
author_sort Mu, Yang
collection PubMed
description Potato is the most important non-grain food in the world, while late blight caused by Phytophthora infestans seriously threatens the production of potato. Since pathogen-associated molecular patterns (PAMPs) are relatively conserved, PAMP-triggered immunity (PTI) can provide durable resistance to late blight for potato. However, knowledge of the regulatory mechanisms of PTI against oomycete pathogens at protein levels remains limited due to the small number of identified proteins. In the present work, changes in the proteome profile of Nicotiana benthamiana leaves upon P. infestans PAMP induction were examined using the SWATH-MS (sequential windowed acquisition of all theoretical mass spectra) approach, which provides quantification of protein abundances and large-scale identification of PTI-related proteins. A total of 4401 proteins have been identified, of which 1429 proteins were differentially expressed at least at one time point of 8, 12, 24 and 48 h after PAMP induction, compared with the expression at 0 h when immediately after PAMP induction. They were further analyzed by expression clustering and gene ontology (GO) enrichment analysis. Through functional verification, six novel DEPs of 19 candidates were proved to be involved in PTI responses, including mitochondrial phosphate carrier protein (MPT) 3, vesicle-associated membrane protein (VAMP) 714, lysophospholipase (LysoPL) 2, ascorbate peroxidase (APX) 1, heat shock 70 kDa protein (HSP) 2 and peptidyl-prolyl cis-trans isomerase FKBP (FKBP) 15-1. Taken together, the time course approach and the resulting large-scale proteomic analyses have enlarged our understanding of PTI mechanisms and provided a valuable resource for the discovery of complex protein networks involved in the resistance response of potato to late blight.
format Online
Article
Text
id pubmed-9715588
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97155882022-12-03 SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans Mu, Yang Guo, Xiao Yu, Jian Wang, Ruxun Liu, Zeng Hu, Kefan Song, Jingyi Chen, Lin Song, Botao Du, Juan Front Plant Sci Plant Science Potato is the most important non-grain food in the world, while late blight caused by Phytophthora infestans seriously threatens the production of potato. Since pathogen-associated molecular patterns (PAMPs) are relatively conserved, PAMP-triggered immunity (PTI) can provide durable resistance to late blight for potato. However, knowledge of the regulatory mechanisms of PTI against oomycete pathogens at protein levels remains limited due to the small number of identified proteins. In the present work, changes in the proteome profile of Nicotiana benthamiana leaves upon P. infestans PAMP induction were examined using the SWATH-MS (sequential windowed acquisition of all theoretical mass spectra) approach, which provides quantification of protein abundances and large-scale identification of PTI-related proteins. A total of 4401 proteins have been identified, of which 1429 proteins were differentially expressed at least at one time point of 8, 12, 24 and 48 h after PAMP induction, compared with the expression at 0 h when immediately after PAMP induction. They were further analyzed by expression clustering and gene ontology (GO) enrichment analysis. Through functional verification, six novel DEPs of 19 candidates were proved to be involved in PTI responses, including mitochondrial phosphate carrier protein (MPT) 3, vesicle-associated membrane protein (VAMP) 714, lysophospholipase (LysoPL) 2, ascorbate peroxidase (APX) 1, heat shock 70 kDa protein (HSP) 2 and peptidyl-prolyl cis-trans isomerase FKBP (FKBP) 15-1. Taken together, the time course approach and the resulting large-scale proteomic analyses have enlarged our understanding of PTI mechanisms and provided a valuable resource for the discovery of complex protein networks involved in the resistance response of potato to late blight. Frontiers Media S.A. 2022-11-18 /pmc/articles/PMC9715588/ /pubmed/36466288 http://dx.doi.org/10.3389/fpls.2022.1036637 Text en Copyright © 2022 Mu, Guo, Yu, Wang, Liu, Hu, Song, Chen, Song and Du https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mu, Yang
Guo, Xiao
Yu, Jian
Wang, Ruxun
Liu, Zeng
Hu, Kefan
Song, Jingyi
Chen, Lin
Song, Botao
Du, Juan
SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title_full SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title_fullStr SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title_full_unstemmed SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title_short SWATH-MS based quantitative proteomics analysis reveals novel proteins involved in PAMP triggered immunity against potato late blight pathogen Phytophthora infestans
title_sort swath-ms based quantitative proteomics analysis reveals novel proteins involved in pamp triggered immunity against potato late blight pathogen phytophthora infestans
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715588/
https://www.ncbi.nlm.nih.gov/pubmed/36466288
http://dx.doi.org/10.3389/fpls.2022.1036637
work_keys_str_mv AT muyang swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT guoxiao swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT yujian swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT wangruxun swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT liuzeng swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT hukefan swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT songjingyi swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT chenlin swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT songbotao swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans
AT dujuan swathmsbasedquantitativeproteomicsanalysisrevealsnovelproteinsinvolvedinpamptriggeredimmunityagainstpotatolateblightpathogenphytophthorainfestans