Cargando…

Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease

Alzheimer’s disease (AD) is a progressive neurological disease that worsens with time. The hallmark illnesses include extracellular senile plaques caused by β-amyloid protein deposition, neurofibrillary tangles caused by tau protein hyperphosphorylation, and neuronal loss accompanying glial cell hyp...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Qi, Chen, Bishuang, Yang, Ping, Wu, Jipan, Pang, Xinping, Pang, Chaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716081/
https://www.ncbi.nlm.nih.gov/pubmed/36468008
http://dx.doi.org/10.3389/fgene.2022.1049786
_version_ 1784842603041128448
author Zhang, Qi
Chen, Bishuang
Yang, Ping
Wu, Jipan
Pang, Xinping
Pang, Chaoyang
author_facet Zhang, Qi
Chen, Bishuang
Yang, Ping
Wu, Jipan
Pang, Xinping
Pang, Chaoyang
author_sort Zhang, Qi
collection PubMed
description Alzheimer’s disease (AD) is a progressive neurological disease that worsens with time. The hallmark illnesses include extracellular senile plaques caused by β-amyloid protein deposition, neurofibrillary tangles caused by tau protein hyperphosphorylation, and neuronal loss accompanying glial cell hyperplasia. Noncoding RNAs are substantially implicated in related pathophysiology, according to mounting data. However, the function of these ncRNAs is mainly unclear. Circular RNAs (circRNAs) include many miRNA-binding sites (miRNA response elements, MREs), which operate as miRNA sponges or competing endogenous RNAs (ceRNAs). The purpose of this study was to look at the role of circular RNAs (circRNAs) and microRNAs (miRNAs) in Alzheimer’s disease (AD) as possible biomarkers. The Gene Expression Omnibus (GEO) database was used to obtain an expression profile of Alzheimer’s disease patients (GSE5281, GSE122603, GSE97760, GSE150693, GSE1297, and GSE161435). Through preliminary data deletion, 163 genes with significant differences, 156 miRNAs with significant differences, and 153 circRNAs with significant differences were identified. Then, 10 key genes, led by MAPT and AP2M1, were identified by the mediation center algorithm, 34 miRNAs with obvious prognosis were identified by the cox regression model, and 16 key circRNAs were selected by the database. To develop competitive endogenous RNA (ceRNA) networks, hub circRNAs and mRNAs were used. Finally, GO analysis and clinical data verification of key genes were carried out. We discovered that a down-regulated circRNA (has_circ_002048) caused the increased expression of numerous miRNAs, which further inhibited the expression of a critical mRNA (AP2M1), leading to Alzheimer’s disease pathology. The findings of this work contribute to a better understanding of the circRNA-miRNA-mRNA regulating processes in Alzheimer’s disease. Furthermore, the ncRNAs found here might become novel biomarkers and potential targets for the development of Alzheimer’s drugs.
format Online
Article
Text
id pubmed-9716081
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97160812022-12-03 Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease Zhang, Qi Chen, Bishuang Yang, Ping Wu, Jipan Pang, Xinping Pang, Chaoyang Front Genet Genetics Alzheimer’s disease (AD) is a progressive neurological disease that worsens with time. The hallmark illnesses include extracellular senile plaques caused by β-amyloid protein deposition, neurofibrillary tangles caused by tau protein hyperphosphorylation, and neuronal loss accompanying glial cell hyperplasia. Noncoding RNAs are substantially implicated in related pathophysiology, according to mounting data. However, the function of these ncRNAs is mainly unclear. Circular RNAs (circRNAs) include many miRNA-binding sites (miRNA response elements, MREs), which operate as miRNA sponges or competing endogenous RNAs (ceRNAs). The purpose of this study was to look at the role of circular RNAs (circRNAs) and microRNAs (miRNAs) in Alzheimer’s disease (AD) as possible biomarkers. The Gene Expression Omnibus (GEO) database was used to obtain an expression profile of Alzheimer’s disease patients (GSE5281, GSE122603, GSE97760, GSE150693, GSE1297, and GSE161435). Through preliminary data deletion, 163 genes with significant differences, 156 miRNAs with significant differences, and 153 circRNAs with significant differences were identified. Then, 10 key genes, led by MAPT and AP2M1, were identified by the mediation center algorithm, 34 miRNAs with obvious prognosis were identified by the cox regression model, and 16 key circRNAs were selected by the database. To develop competitive endogenous RNA (ceRNA) networks, hub circRNAs and mRNAs were used. Finally, GO analysis and clinical data verification of key genes were carried out. We discovered that a down-regulated circRNA (has_circ_002048) caused the increased expression of numerous miRNAs, which further inhibited the expression of a critical mRNA (AP2M1), leading to Alzheimer’s disease pathology. The findings of this work contribute to a better understanding of the circRNA-miRNA-mRNA regulating processes in Alzheimer’s disease. Furthermore, the ncRNAs found here might become novel biomarkers and potential targets for the development of Alzheimer’s drugs. Frontiers Media S.A. 2022-11-18 /pmc/articles/PMC9716081/ /pubmed/36468008 http://dx.doi.org/10.3389/fgene.2022.1049786 Text en Copyright © 2022 Zhang, Chen, Yang, Wu, Pang and Pang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Qi
Chen, Bishuang
Yang, Ping
Wu, Jipan
Pang, Xinping
Pang, Chaoyang
Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title_full Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title_fullStr Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title_full_unstemmed Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title_short Bioinformatics-based study reveals that AP2M1 is regulated by the circRNA-miRNA-mRNA interaction network and affects Alzheimer’s disease
title_sort bioinformatics-based study reveals that ap2m1 is regulated by the circrna-mirna-mrna interaction network and affects alzheimer’s disease
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716081/
https://www.ncbi.nlm.nih.gov/pubmed/36468008
http://dx.doi.org/10.3389/fgene.2022.1049786
work_keys_str_mv AT zhangqi bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease
AT chenbishuang bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease
AT yangping bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease
AT wujipan bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease
AT pangxinping bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease
AT pangchaoyang bioinformaticsbasedstudyrevealsthatap2m1isregulatedbythecircrnamirnamrnainteractionnetworkandaffectsalzheimersdisease