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Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model

Islet β-cell biomarkers can reflect changes in the number and function of islet β-cells in the prediabetes or early diabetes stage. CD34 is a commonly used stem cell biomarker; however, its expression and function in pancreatic islets remain unclear. In the present study, double immunofluorescence s...

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Autores principales: Huang, Shichen, Li, Zhiyuan, Sun, Yuhan, Chen, Baiyi, Jiang, Yuxin, Hong, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716098/
https://www.ncbi.nlm.nih.gov/pubmed/36467676
http://dx.doi.org/10.3389/fphys.2022.1032774
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author Huang, Shichen
Li, Zhiyuan
Sun, Yuhan
Chen, Baiyi
Jiang, Yuxin
Hong, Feng
author_facet Huang, Shichen
Li, Zhiyuan
Sun, Yuhan
Chen, Baiyi
Jiang, Yuxin
Hong, Feng
author_sort Huang, Shichen
collection PubMed
description Islet β-cell biomarkers can reflect changes in the number and function of islet β-cells in the prediabetes or early diabetes stage. CD34 is a commonly used stem cell biomarker; however, its expression and function in pancreatic islets remain unclear. In the present study, double immunofluorescence staining, proteomic bioinformatics analysis, and correlation analysis were used to explore the potential of CD34 as an islet β-cell biomarker. Bioinformatics analysis revealed that the amino acid sequence of CD34 was conserved among multiple species and abundantly expressed on mouse and human pancreatic tissues. Immunofluorescence demonstrated that in the control rat pancreas, CD34 was expressed on glucagon-labeled islet α-cells but not on insulin-labeled islet β-cells. Furthermore, the proportion of CD34-positive cells, which were also positive for glucagon, was significantly increased in alloxan-induced diabetes models. Statistical analysis revealed that the expression of CD34 was negatively correlated with the number of insulin-labeled islet β-cells during diabetes progression in dose-dependent fashion in alloxan-induced diabetes models. Furthermore, the results suggested that the transdifferentiation of islet β-cells into islet α-cells may occur in the process of diabetes. Thus, the present study demonstrated that CD34 is expressed on islet α-cells, and its number is linearly and negatively correlated with the number of islet β-cells, suggesting that CD34 can be used as a prospective biomarker for islet β-cells in the early diagnosis of diabetes. The study also suggests the transformation of β-cells to α-cells in diabetes which provide a potential to be applied towards diabetes mechanism research.
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spelling pubmed-97160982022-12-03 Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model Huang, Shichen Li, Zhiyuan Sun, Yuhan Chen, Baiyi Jiang, Yuxin Hong, Feng Front Physiol Physiology Islet β-cell biomarkers can reflect changes in the number and function of islet β-cells in the prediabetes or early diabetes stage. CD34 is a commonly used stem cell biomarker; however, its expression and function in pancreatic islets remain unclear. In the present study, double immunofluorescence staining, proteomic bioinformatics analysis, and correlation analysis were used to explore the potential of CD34 as an islet β-cell biomarker. Bioinformatics analysis revealed that the amino acid sequence of CD34 was conserved among multiple species and abundantly expressed on mouse and human pancreatic tissues. Immunofluorescence demonstrated that in the control rat pancreas, CD34 was expressed on glucagon-labeled islet α-cells but not on insulin-labeled islet β-cells. Furthermore, the proportion of CD34-positive cells, which were also positive for glucagon, was significantly increased in alloxan-induced diabetes models. Statistical analysis revealed that the expression of CD34 was negatively correlated with the number of insulin-labeled islet β-cells during diabetes progression in dose-dependent fashion in alloxan-induced diabetes models. Furthermore, the results suggested that the transdifferentiation of islet β-cells into islet α-cells may occur in the process of diabetes. Thus, the present study demonstrated that CD34 is expressed on islet α-cells, and its number is linearly and negatively correlated with the number of islet β-cells, suggesting that CD34 can be used as a prospective biomarker for islet β-cells in the early diagnosis of diabetes. The study also suggests the transformation of β-cells to α-cells in diabetes which provide a potential to be applied towards diabetes mechanism research. Frontiers Media S.A. 2022-11-18 /pmc/articles/PMC9716098/ /pubmed/36467676 http://dx.doi.org/10.3389/fphys.2022.1032774 Text en Copyright © 2022 Huang, Li, Sun, Chen, Jiang and Hong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Huang, Shichen
Li, Zhiyuan
Sun, Yuhan
Chen, Baiyi
Jiang, Yuxin
Hong, Feng
Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title_full Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title_fullStr Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title_full_unstemmed Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title_short Increased CD34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
title_sort increased cd34 in pancreatic islet negatively predict islet β-cell decrease in type1 diabetes model
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716098/
https://www.ncbi.nlm.nih.gov/pubmed/36467676
http://dx.doi.org/10.3389/fphys.2022.1032774
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