Cargando…

Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury

OBJECTIVE: To evaluate the mechanism of Bone Marrow Mesenchymal Stem Cells (BMSCs) in regulating NF-κB signal pathway by targeting miR-449a. METHODS: Stem cells were transfected by over-expressing and inhibiting miR-449a to detect the levels and viability of miR-449a in stem cells after transfection...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Hongjiao, Wang, Fangyuan, Wang, Yuejing, Li, Xiaonan, Di, Cihan, Liang, Chunming, Mu, Yuyuan, Zhou, Jiexin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society of Musculoskeletal and Neuronal Interactions 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716300/
https://www.ncbi.nlm.nih.gov/pubmed/36458392
_version_ 1784842657142407168
author Wang, Hongjiao
Wang, Fangyuan
Wang, Yuejing
Li, Xiaonan
Di, Cihan
Liang, Chunming
Mu, Yuyuan
Zhou, Jiexin
author_facet Wang, Hongjiao
Wang, Fangyuan
Wang, Yuejing
Li, Xiaonan
Di, Cihan
Liang, Chunming
Mu, Yuyuan
Zhou, Jiexin
author_sort Wang, Hongjiao
collection PubMed
description OBJECTIVE: To evaluate the mechanism of Bone Marrow Mesenchymal Stem Cells (BMSCs) in regulating NF-κB signal pathway by targeting miR-449a. METHODS: Stem cells were transfected by over-expressing and inhibiting miR-449a to detect the levels and viability of miR-449a in stem cells after transfection. Stem cells and neurons were co-cultured in vitro to evaluate the in vitro mechanism of stem cells over-expressing miR-449a on neurons. RESULTS: After the addition of neurons, the neuronal activity of miR-449a over-expression group increased significantly, the expression of NF-κB signal pathway proteins (IκBα, p50, and p65) decreased, and the inflammatory cytokines (TNF-α and IL-1β) decreased significantly (P<0.05). In vivo experiments in rats also showed that rats were unresponsive, did not chirp or elude after being stimulated. After stem cell therapy, the weight and response of rats gradually returned to normal levels. miR-449a expression significantly increased in the stem cell + miR-449a over-expression group, expression of NF-κB signal pathway proteins (IκBα, p50, and p65) decreased, inflammatory cytokines (TNF-α and IL-1β) significantly decreased, and cell activity significantly increased (P<0.05). CONCLUSIONS: BMSCs can modulate NF-κB signaling pathway by targeting miR-449a, so as to reduce the inflammatory response to peripheral nerve injury and repair nerve injury.
format Online
Article
Text
id pubmed-9716300
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher International Society of Musculoskeletal and Neuronal Interactions
record_format MEDLINE/PubMed
spelling pubmed-97163002022-12-07 Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury Wang, Hongjiao Wang, Fangyuan Wang, Yuejing Li, Xiaonan Di, Cihan Liang, Chunming Mu, Yuyuan Zhou, Jiexin J Musculoskelet Neuronal Interact Original Article OBJECTIVE: To evaluate the mechanism of Bone Marrow Mesenchymal Stem Cells (BMSCs) in regulating NF-κB signal pathway by targeting miR-449a. METHODS: Stem cells were transfected by over-expressing and inhibiting miR-449a to detect the levels and viability of miR-449a in stem cells after transfection. Stem cells and neurons were co-cultured in vitro to evaluate the in vitro mechanism of stem cells over-expressing miR-449a on neurons. RESULTS: After the addition of neurons, the neuronal activity of miR-449a over-expression group increased significantly, the expression of NF-κB signal pathway proteins (IκBα, p50, and p65) decreased, and the inflammatory cytokines (TNF-α and IL-1β) decreased significantly (P<0.05). In vivo experiments in rats also showed that rats were unresponsive, did not chirp or elude after being stimulated. After stem cell therapy, the weight and response of rats gradually returned to normal levels. miR-449a expression significantly increased in the stem cell + miR-449a over-expression group, expression of NF-κB signal pathway proteins (IκBα, p50, and p65) decreased, inflammatory cytokines (TNF-α and IL-1β) significantly decreased, and cell activity significantly increased (P<0.05). CONCLUSIONS: BMSCs can modulate NF-κB signaling pathway by targeting miR-449a, so as to reduce the inflammatory response to peripheral nerve injury and repair nerve injury. International Society of Musculoskeletal and Neuronal Interactions 2022 /pmc/articles/PMC9716300/ /pubmed/36458392 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wang, Hongjiao
Wang, Fangyuan
Wang, Yuejing
Li, Xiaonan
Di, Cihan
Liang, Chunming
Mu, Yuyuan
Zhou, Jiexin
Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title_full Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title_fullStr Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title_full_unstemmed Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title_short Study on the Mechanism of BMSCs in Regulating NF-κB Signal Pathway by Targeting miR-449a to Improve the Inflammatory Response to Peripheral Nerve Injury
title_sort study on the mechanism of bmscs in regulating nf-κb signal pathway by targeting mir-449a to improve the inflammatory response to peripheral nerve injury
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716300/
https://www.ncbi.nlm.nih.gov/pubmed/36458392
work_keys_str_mv AT wanghongjiao studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT wangfangyuan studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT wangyuejing studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT lixiaonan studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT dicihan studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT liangchunming studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT muyuyuan studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury
AT zhoujiexin studyonthemechanismofbmscsinregulatingnfkbsignalpathwaybytargetingmir449atoimprovetheinflammatoryresponsetoperipheralnerveinjury