Cargando…

Establishment of a mouse model for bile duct repair and tissue engineering

Due to the lack of a suitable model, research on biliary biology is far behind that on other organs. A mouse model of common bile duct (CBD) dilation (BDD) was first established and compared with CBD ligation mice (BDL). Then, in a transplantation experiment, the dilated CBD of recipient BDD mice wa...

Descripción completa

Detalles Bibliográficos
Autores principales: Ge, Xinlan, Xu, Dabin, Pan, Ke, Mao, Guankun, Lu, Shichun, Li, Chonghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716339/
https://www.ncbi.nlm.nih.gov/pubmed/36478886
http://dx.doi.org/10.3892/etm.2022.11675
_version_ 1784842666764140544
author Ge, Xinlan
Xu, Dabin
Pan, Ke
Mao, Guankun
Lu, Shichun
Li, Chonghui
author_facet Ge, Xinlan
Xu, Dabin
Pan, Ke
Mao, Guankun
Lu, Shichun
Li, Chonghui
author_sort Ge, Xinlan
collection PubMed
description Due to the lack of a suitable model, research on biliary biology is far behind that on other organs. A mouse model of common bile duct (CBD) dilation (BDD) was first established and compared with CBD ligation mice (BDL). Then, in a transplantation experiment, the dilated CBD of recipient BDD mice was injured by making an elliptical incision and repaired by transplanting a bile duct patch from donor BDD mice. Biochemical and histological changes were analyzed and cell proliferation of the bile duct grafts was determined. Slightly dilated and unblocked CBD with a diameter of 2.89±0.76 mm was obtained in BDD mice, while the CBD diameter was 0.51±0.08 mm in the Sham group and 4.71±0.64 mm in the BDL group on day 14 after surgery. The liver damage was very mild in BDD mice compared with BDL mice, proving that the BDD model could be further used for bile duct transplantation. By cross transplanting the bile duct patch from enhanced green fluorescence protein and wild-type BDD mice, it was found that the CBD injury was well repaired and the cells of the bile duct patch were completely replaced by recipient-derived cells at 12 week after the repair operation. α Smooth muscle actin, Ki67 and cytokeratin 19 immunofluorescence staining showed that the proliferation of bile duct epithelial cells and abundant active fibroblasts were found within the bile duct patch during the regeneration process. Therefore, a reliable new mouse model of bile duct injury and repair was successfully established and can be used in the study of biliary repair mechanisms and tissue engineering of biliary ducts.
format Online
Article
Text
id pubmed-9716339
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-97163392022-12-06 Establishment of a mouse model for bile duct repair and tissue engineering Ge, Xinlan Xu, Dabin Pan, Ke Mao, Guankun Lu, Shichun Li, Chonghui Exp Ther Med Articles Due to the lack of a suitable model, research on biliary biology is far behind that on other organs. A mouse model of common bile duct (CBD) dilation (BDD) was first established and compared with CBD ligation mice (BDL). Then, in a transplantation experiment, the dilated CBD of recipient BDD mice was injured by making an elliptical incision and repaired by transplanting a bile duct patch from donor BDD mice. Biochemical and histological changes were analyzed and cell proliferation of the bile duct grafts was determined. Slightly dilated and unblocked CBD with a diameter of 2.89±0.76 mm was obtained in BDD mice, while the CBD diameter was 0.51±0.08 mm in the Sham group and 4.71±0.64 mm in the BDL group on day 14 after surgery. The liver damage was very mild in BDD mice compared with BDL mice, proving that the BDD model could be further used for bile duct transplantation. By cross transplanting the bile duct patch from enhanced green fluorescence protein and wild-type BDD mice, it was found that the CBD injury was well repaired and the cells of the bile duct patch were completely replaced by recipient-derived cells at 12 week after the repair operation. α Smooth muscle actin, Ki67 and cytokeratin 19 immunofluorescence staining showed that the proliferation of bile duct epithelial cells and abundant active fibroblasts were found within the bile duct patch during the regeneration process. Therefore, a reliable new mouse model of bile duct injury and repair was successfully established and can be used in the study of biliary repair mechanisms and tissue engineering of biliary ducts. D.A. Spandidos 2022-11-02 /pmc/articles/PMC9716339/ /pubmed/36478886 http://dx.doi.org/10.3892/etm.2022.11675 Text en Copyright: © Ge et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ge, Xinlan
Xu, Dabin
Pan, Ke
Mao, Guankun
Lu, Shichun
Li, Chonghui
Establishment of a mouse model for bile duct repair and tissue engineering
title Establishment of a mouse model for bile duct repair and tissue engineering
title_full Establishment of a mouse model for bile duct repair and tissue engineering
title_fullStr Establishment of a mouse model for bile duct repair and tissue engineering
title_full_unstemmed Establishment of a mouse model for bile duct repair and tissue engineering
title_short Establishment of a mouse model for bile duct repair and tissue engineering
title_sort establishment of a mouse model for bile duct repair and tissue engineering
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716339/
https://www.ncbi.nlm.nih.gov/pubmed/36478886
http://dx.doi.org/10.3892/etm.2022.11675
work_keys_str_mv AT gexinlan establishmentofamousemodelforbileductrepairandtissueengineering
AT xudabin establishmentofamousemodelforbileductrepairandtissueengineering
AT panke establishmentofamousemodelforbileductrepairandtissueengineering
AT maoguankun establishmentofamousemodelforbileductrepairandtissueengineering
AT lushichun establishmentofamousemodelforbileductrepairandtissueengineering
AT lichonghui establishmentofamousemodelforbileductrepairandtissueengineering