Cargando…
Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity
BACKGROUND: Moringa concanensis Nimmo (MC), a plant that resembles Moringa oleifera Lam. (MO), has less scientific information but has traditionally been used as a medicinal plant. Moringa species have long been known for their medicinal qualities, which include antioxidant, anti-inflammatory, antic...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9717441/ https://www.ncbi.nlm.nih.gov/pubmed/36460949 http://dx.doi.org/10.1186/s12870-022-03938-6 |
_version_ | 1784842902067740672 |
---|---|
author | Shafi, K. Mohamed Sajeevan, Radha Sivarajan Kouser, Sania Vishnuprasad, Chethala N Sowdhamini, Ramanathan |
author_facet | Shafi, K. Mohamed Sajeevan, Radha Sivarajan Kouser, Sania Vishnuprasad, Chethala N Sowdhamini, Ramanathan |
author_sort | Shafi, K. Mohamed |
collection | PubMed |
description | BACKGROUND: Moringa concanensis Nimmo (MC), a plant that resembles Moringa oleifera Lam. (MO), has less scientific information but has traditionally been used as a medicinal plant. Moringa species have long been known for their medicinal qualities, which include antioxidant, anti-inflammatory, anticancer, and antihyperglycemic effects. We investigated the antidiabetic potential of MC and MO species in this study by using transcriptome profiling, metabolite analysis, and in vitro assay studies. RESULTS: Our transcriptome analysis revealed the expression of enzymes involved in the biosynthesis of quercetin, chlorogenic acid, and benzylamine, all of which have previously been shown to have antidiabetic activity. We compared the expression patterns of five different tissues from MC and MO and it was found that the key enzymes involved in the biosynthesis of these compounds were highly expressed in leaf tissue. The expression estimated by MC transcriptome data in different tissues was verified using RT-qPCR analysis. The amount of these compounds was further quantified in the crude leaf extract of both species and found that MC had a higher abundance of quercetin and chlorogenic acid than MO. The crude leaf extract from both MC and MO were further tested in vitro, and the results demonstrated strong inhibitory activity for α-glucosidase and DPP-IV enzymes. Our findings suggest that compounds in leaf tissue, such as quercetin, benzylamine, and chlorogenic acid, could play a significant role in this antidiabetic activity. In addition, when comparing MO plants, we found that MC had a slightly higher effect in expression, abundance, and inhibitory activity. CONCLUSIONS: This study presents the first report of MC transcriptome data, as well as a comparison of its anti-diabetic activity to MO. Our analysis discussed the significance of leaf tissue in antidiabetic activity compared to other tissues of both species. Overall, this study not only provides transcriptome resources for Moringa species, but also sheds light on antidiabetic potential of both species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03938-6. |
format | Online Article Text |
id | pubmed-9717441 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97174412022-12-03 Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity Shafi, K. Mohamed Sajeevan, Radha Sivarajan Kouser, Sania Vishnuprasad, Chethala N Sowdhamini, Ramanathan BMC Plant Biol Research BACKGROUND: Moringa concanensis Nimmo (MC), a plant that resembles Moringa oleifera Lam. (MO), has less scientific information but has traditionally been used as a medicinal plant. Moringa species have long been known for their medicinal qualities, which include antioxidant, anti-inflammatory, anticancer, and antihyperglycemic effects. We investigated the antidiabetic potential of MC and MO species in this study by using transcriptome profiling, metabolite analysis, and in vitro assay studies. RESULTS: Our transcriptome analysis revealed the expression of enzymes involved in the biosynthesis of quercetin, chlorogenic acid, and benzylamine, all of which have previously been shown to have antidiabetic activity. We compared the expression patterns of five different tissues from MC and MO and it was found that the key enzymes involved in the biosynthesis of these compounds were highly expressed in leaf tissue. The expression estimated by MC transcriptome data in different tissues was verified using RT-qPCR analysis. The amount of these compounds was further quantified in the crude leaf extract of both species and found that MC had a higher abundance of quercetin and chlorogenic acid than MO. The crude leaf extract from both MC and MO were further tested in vitro, and the results demonstrated strong inhibitory activity for α-glucosidase and DPP-IV enzymes. Our findings suggest that compounds in leaf tissue, such as quercetin, benzylamine, and chlorogenic acid, could play a significant role in this antidiabetic activity. In addition, when comparing MO plants, we found that MC had a slightly higher effect in expression, abundance, and inhibitory activity. CONCLUSIONS: This study presents the first report of MC transcriptome data, as well as a comparison of its anti-diabetic activity to MO. Our analysis discussed the significance of leaf tissue in antidiabetic activity compared to other tissues of both species. Overall, this study not only provides transcriptome resources for Moringa species, but also sheds light on antidiabetic potential of both species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03938-6. BioMed Central 2022-12-02 /pmc/articles/PMC9717441/ /pubmed/36460949 http://dx.doi.org/10.1186/s12870-022-03938-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Shafi, K. Mohamed Sajeevan, Radha Sivarajan Kouser, Sania Vishnuprasad, Chethala N Sowdhamini, Ramanathan Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title | Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title_full | Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title_fullStr | Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title_full_unstemmed | Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title_short | Transcriptome profiling of two Moringa species and insights into their antihyperglycemic activity |
title_sort | transcriptome profiling of two moringa species and insights into their antihyperglycemic activity |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9717441/ https://www.ncbi.nlm.nih.gov/pubmed/36460949 http://dx.doi.org/10.1186/s12870-022-03938-6 |
work_keys_str_mv | AT shafikmohamed transcriptomeprofilingoftwomoringaspeciesandinsightsintotheirantihyperglycemicactivity AT sajeevanradhasivarajan transcriptomeprofilingoftwomoringaspeciesandinsightsintotheirantihyperglycemicactivity AT kousersania transcriptomeprofilingoftwomoringaspeciesandinsightsintotheirantihyperglycemicactivity AT vishnuprasadchethalan transcriptomeprofilingoftwomoringaspeciesandinsightsintotheirantihyperglycemicactivity AT sowdhaminiramanathan transcriptomeprofilingoftwomoringaspeciesandinsightsintotheirantihyperglycemicactivity |