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Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study

OBJECTIVE: To examine the plasma microbiome for differences between obese individuals with and without osteoarthritis (OA) and its association with serum lipopolysaccharide (LPS). DESIGN: Blood samples from 70 participants with body mass index (BMI) ​≥ ​30kg/m2 and age ≥55 years, with (cases) or wit...

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Autores principales: Arbeeva, Liubov, Azcarate-Peril, M. Andrea, Cui, Yang, Nelson, Amanda E., Loeser, Richard F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718202/
https://www.ncbi.nlm.nih.gov/pubmed/36474790
http://dx.doi.org/10.1016/j.ocarto.2022.100317
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author Arbeeva, Liubov
Azcarate-Peril, M. Andrea
Cui, Yang
Nelson, Amanda E.
Loeser, Richard F.
author_facet Arbeeva, Liubov
Azcarate-Peril, M. Andrea
Cui, Yang
Nelson, Amanda E.
Loeser, Richard F.
author_sort Arbeeva, Liubov
collection PubMed
description OBJECTIVE: To examine the plasma microbiome for differences between obese individuals with and without osteoarthritis (OA) and its association with serum lipopolysaccharide (LPS). DESIGN: Blood samples from 70 participants with body mass index (BMI) ​≥ ​30kg/m2 and age ≥55 years, with (cases) or without (controls) hand plus knee OA, were analyzed for serum LPS and composition of the plasma microbiome. The Dirichlet-multinominal recursive partitioning model (DM-RPart) was applied to microbiome compositional data to test the hypothesis that LPS levels distinguish plasma microbiome, accounting for BMI and age. RESULTS: No significant differences in alpha diversity, or compositional differences between groups at the genus level, were seen between cases and controls (p ​= ​0.11). β-Diversity was significantly associated with serum LPS levels (p ​= ​0.01). DM-RPart resulted in an optimal tree with 3 divisions: 1) based on age (split at 69 years); 2) those older than 69 were split based on BMI; 3) those with BMI <39 ​kg/m2 were split based on LPS level (at 65 EU/ml). This resulted in 4 groups (nodes 2, and 5–7). Participants in node 2 were younger and the majority had no or mild OA. Those in nodes 5 and 6 were comparable in age and BMI but node 6 had higher LPS and more severe OA. Individuals in node 7 were older, had higher BMI, and the most severe OA. CONCLUSIONS: Our results suggest a relationship between serum LPS and the plasma microbiome in a subgroup of obese individuals with hand plus knee OA that could reflect differences in intestinal permeability.
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spelling pubmed-97182022022-12-05 Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study Arbeeva, Liubov Azcarate-Peril, M. Andrea Cui, Yang Nelson, Amanda E. Loeser, Richard F. Osteoarthr Cartil Open Brief Report OBJECTIVE: To examine the plasma microbiome for differences between obese individuals with and without osteoarthritis (OA) and its association with serum lipopolysaccharide (LPS). DESIGN: Blood samples from 70 participants with body mass index (BMI) ​≥ ​30kg/m2 and age ≥55 years, with (cases) or without (controls) hand plus knee OA, were analyzed for serum LPS and composition of the plasma microbiome. The Dirichlet-multinominal recursive partitioning model (DM-RPart) was applied to microbiome compositional data to test the hypothesis that LPS levels distinguish plasma microbiome, accounting for BMI and age. RESULTS: No significant differences in alpha diversity, or compositional differences between groups at the genus level, were seen between cases and controls (p ​= ​0.11). β-Diversity was significantly associated with serum LPS levels (p ​= ​0.01). DM-RPart resulted in an optimal tree with 3 divisions: 1) based on age (split at 69 years); 2) those older than 69 were split based on BMI; 3) those with BMI <39 ​kg/m2 were split based on LPS level (at 65 EU/ml). This resulted in 4 groups (nodes 2, and 5–7). Participants in node 2 were younger and the majority had no or mild OA. Those in nodes 5 and 6 were comparable in age and BMI but node 6 had higher LPS and more severe OA. Individuals in node 7 were older, had higher BMI, and the most severe OA. CONCLUSIONS: Our results suggest a relationship between serum LPS and the plasma microbiome in a subgroup of obese individuals with hand plus knee OA that could reflect differences in intestinal permeability. Elsevier 2022-10-29 /pmc/articles/PMC9718202/ /pubmed/36474790 http://dx.doi.org/10.1016/j.ocarto.2022.100317 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Brief Report
Arbeeva, Liubov
Azcarate-Peril, M. Andrea
Cui, Yang
Nelson, Amanda E.
Loeser, Richard F.
Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title_full Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title_fullStr Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title_full_unstemmed Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title_short Association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: An exploratory study
title_sort association of plasma microbial composition with a leaky gut in obesity-related osteoarthritis: an exploratory study
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718202/
https://www.ncbi.nlm.nih.gov/pubmed/36474790
http://dx.doi.org/10.1016/j.ocarto.2022.100317
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