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Disuse histological changes of an unloading environment on joint components in rat knee joints
OBJECTIVES: To clarify the effects of mechanical unloading on joint components, including the articular cartilage by knee compartments, synovial membrane, and the infrapatellar fat pad (IFP), using a hindlimb suspension rat model. DESIGN: Twenty-five male rats were divided into the three following e...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718232/ https://www.ncbi.nlm.nih.gov/pubmed/36474725 http://dx.doi.org/10.1016/j.ocarto.2019.100008 |
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author | Takahashi, Ikufumi Matsuzaki, Taro Kuroki, Hiroshi Hoso, Masahiro |
author_facet | Takahashi, Ikufumi Matsuzaki, Taro Kuroki, Hiroshi Hoso, Masahiro |
author_sort | Takahashi, Ikufumi |
collection | PubMed |
description | OBJECTIVES: To clarify the effects of mechanical unloading on joint components, including the articular cartilage by knee compartments, synovial membrane, and the infrapatellar fat pad (IFP), using a hindlimb suspension rat model. DESIGN: Twenty-five male rats were divided into the three following experimental groups: the baseline, control (CON), and hindlimb unloading (HU) groups. Rats in the HU group were subjected to periods of hindlimb unloading by tail suspension. The joint components were evaluated by histopathological and histomorphometric analyses. RESULTS: The unloading condition caused articular cartilage thinning and decreased matrix staining. The tendency of these histological changes in articular cartilage was similar between the knee compartments. In general, the unloading environment resulted in no critical histological changes to the synovial membrane and IFP. At 4 weeks, thickening of the synovial membrane and synovial-like tissue invasion were observed in the HU group, but there was no significant change in inflammation. There was no obvious histological change to the IFP caused by unloading. CONCLUSION: The unloading environment caused disuse histological changes in the articular cartilage; however, the tendency of the changes was similar between compartments. Furthermore, the unloading condition caused no critical histological changes to the synovial membrane or IFP. Clinically, the unloading environment without joint immobilization over a short period may not have caused critical histological changes to the joint components. |
format | Online Article Text |
id | pubmed-9718232 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-97182322022-12-05 Disuse histological changes of an unloading environment on joint components in rat knee joints Takahashi, Ikufumi Matsuzaki, Taro Kuroki, Hiroshi Hoso, Masahiro Osteoarthr Cartil Open Original Paper OBJECTIVES: To clarify the effects of mechanical unloading on joint components, including the articular cartilage by knee compartments, synovial membrane, and the infrapatellar fat pad (IFP), using a hindlimb suspension rat model. DESIGN: Twenty-five male rats were divided into the three following experimental groups: the baseline, control (CON), and hindlimb unloading (HU) groups. Rats in the HU group were subjected to periods of hindlimb unloading by tail suspension. The joint components were evaluated by histopathological and histomorphometric analyses. RESULTS: The unloading condition caused articular cartilage thinning and decreased matrix staining. The tendency of these histological changes in articular cartilage was similar between the knee compartments. In general, the unloading environment resulted in no critical histological changes to the synovial membrane and IFP. At 4 weeks, thickening of the synovial membrane and synovial-like tissue invasion were observed in the HU group, but there was no significant change in inflammation. There was no obvious histological change to the IFP caused by unloading. CONCLUSION: The unloading environment caused disuse histological changes in the articular cartilage; however, the tendency of the changes was similar between compartments. Furthermore, the unloading condition caused no critical histological changes to the synovial membrane or IFP. Clinically, the unloading environment without joint immobilization over a short period may not have caused critical histological changes to the joint components. Elsevier 2019-11-13 /pmc/articles/PMC9718232/ /pubmed/36474725 http://dx.doi.org/10.1016/j.ocarto.2019.100008 Text en © 2019 The Authors. Published by Elsevier Ltd on behalf of Osteoarthritis Research Society International (OARSI). https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Paper Takahashi, Ikufumi Matsuzaki, Taro Kuroki, Hiroshi Hoso, Masahiro Disuse histological changes of an unloading environment on joint components in rat knee joints |
title | Disuse histological changes of an unloading environment on joint components in rat knee joints |
title_full | Disuse histological changes of an unloading environment on joint components in rat knee joints |
title_fullStr | Disuse histological changes of an unloading environment on joint components in rat knee joints |
title_full_unstemmed | Disuse histological changes of an unloading environment on joint components in rat knee joints |
title_short | Disuse histological changes of an unloading environment on joint components in rat knee joints |
title_sort | disuse histological changes of an unloading environment on joint components in rat knee joints |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718232/ https://www.ncbi.nlm.nih.gov/pubmed/36474725 http://dx.doi.org/10.1016/j.ocarto.2019.100008 |
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