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The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice

OBJECTIVE: While a role for vitamin K in maintaining joint tissue homeostasis has been proposed based on the presence of vitamin K dependent proteins in cartilage and bone, it is not clear if low vitamin K intake is causally linked to joint tissue degeneration. To address this gap, we manipulated vi...

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Autores principales: Shea, M. Kyla, Booth, Sarah L., Harshman, Stephanie G., Smith, Donald, Carlson, Cathy S., Harper, Lindsey, Armstrong, Alexandra R., Fang, Min, Cancela, M. Leonor, Márcio Simão, Loeser, Richard F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718348/
https://www.ncbi.nlm.nih.gov/pubmed/36474686
http://dx.doi.org/10.1016/j.ocarto.2020.100078
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author Shea, M. Kyla
Booth, Sarah L.
Harshman, Stephanie G.
Smith, Donald
Carlson, Cathy S.
Harper, Lindsey
Armstrong, Alexandra R.
Fang, Min
Cancela, M. Leonor
Márcio Simão
Loeser, Richard F.
author_facet Shea, M. Kyla
Booth, Sarah L.
Harshman, Stephanie G.
Smith, Donald
Carlson, Cathy S.
Harper, Lindsey
Armstrong, Alexandra R.
Fang, Min
Cancela, M. Leonor
Márcio Simão
Loeser, Richard F.
author_sort Shea, M. Kyla
collection PubMed
description OBJECTIVE: While a role for vitamin K in maintaining joint tissue homeostasis has been proposed based on the presence of vitamin K dependent proteins in cartilage and bone, it is not clear if low vitamin K intake is causally linked to joint tissue degeneration. To address this gap, we manipulated vitamin K status in aging mice to test its effect on age-related changes in articular cartilage and sub-chondral bone. METHODS: Eleven-month old male C57BL6 mice were randomly assigned to a low vitamin K diet containing 120 mcg phylloquinone/kg diet (n = 32) or a control diet containing 1.5 mg phylloquinone/kg diet (n = 30) for 6 months. Knees were evaluated histologically using Safranin O and H&E staining, as well as using micro-CT. RESULTS: Eleven mice in the low vitamin K diet group and three mice in the control group died within the first 100 days of the experiment (p = 0.024). Mice fed the low vitamin K diet had higher Safranin-O scores, indicative of more proteoglycan loss, compared to mice fed the control diet (p ≤ 0.026). The articular cartilage structure scores did not differ between the two groups (p ≥ 0.190). The sub-chondral bone parameters measured using micro CT also did not differ between the two groups (all p ≥ 0.174). CONCLUSION: Our findings suggest low vitamin K status can promote joint tissue proteoglycan loss in older male mice. Future studies are needed to confirm our findings and obtain a better understanding of the molecular mechanisms underlying the role of vitamin K in joint tissue homeostasis.
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spelling pubmed-97183482022-12-05 The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice Shea, M. Kyla Booth, Sarah L. Harshman, Stephanie G. Smith, Donald Carlson, Cathy S. Harper, Lindsey Armstrong, Alexandra R. Fang, Min Cancela, M. Leonor Márcio Simão Loeser, Richard F. Osteoarthr Cartil Open ORIGINAL PAPER OBJECTIVE: While a role for vitamin K in maintaining joint tissue homeostasis has been proposed based on the presence of vitamin K dependent proteins in cartilage and bone, it is not clear if low vitamin K intake is causally linked to joint tissue degeneration. To address this gap, we manipulated vitamin K status in aging mice to test its effect on age-related changes in articular cartilage and sub-chondral bone. METHODS: Eleven-month old male C57BL6 mice were randomly assigned to a low vitamin K diet containing 120 mcg phylloquinone/kg diet (n = 32) or a control diet containing 1.5 mg phylloquinone/kg diet (n = 30) for 6 months. Knees were evaluated histologically using Safranin O and H&E staining, as well as using micro-CT. RESULTS: Eleven mice in the low vitamin K diet group and three mice in the control group died within the first 100 days of the experiment (p = 0.024). Mice fed the low vitamin K diet had higher Safranin-O scores, indicative of more proteoglycan loss, compared to mice fed the control diet (p ≤ 0.026). The articular cartilage structure scores did not differ between the two groups (p ≥ 0.190). The sub-chondral bone parameters measured using micro CT also did not differ between the two groups (all p ≥ 0.174). CONCLUSION: Our findings suggest low vitamin K status can promote joint tissue proteoglycan loss in older male mice. Future studies are needed to confirm our findings and obtain a better understanding of the molecular mechanisms underlying the role of vitamin K in joint tissue homeostasis. Elsevier 2020-06-02 /pmc/articles/PMC9718348/ /pubmed/36474686 http://dx.doi.org/10.1016/j.ocarto.2020.100078 Text en © 2020 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle ORIGINAL PAPER
Shea, M. Kyla
Booth, Sarah L.
Harshman, Stephanie G.
Smith, Donald
Carlson, Cathy S.
Harper, Lindsey
Armstrong, Alexandra R.
Fang, Min
Cancela, M. Leonor
Márcio Simão
Loeser, Richard F.
The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title_full The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title_fullStr The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title_full_unstemmed The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title_short The effect of vitamin K insufficiency on histological and structural properties of knee joints in aging mice
title_sort effect of vitamin k insufficiency on histological and structural properties of knee joints in aging mice
topic ORIGINAL PAPER
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718348/
https://www.ncbi.nlm.nih.gov/pubmed/36474686
http://dx.doi.org/10.1016/j.ocarto.2020.100078
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