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Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China

Yinqiao powder, with significant anti‐inflammatory and antiviral effects, is a classical formula for the treatment of febrile diseases in China. During the SARS period in 2003, Yinqiao powder showed a good antipyretic effect. It also plays a major role in the treatment for COVID-19 in China. Althoug...

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Detalles Bibliográficos
Autores principales: Zhang, Huimin, Xu, Lin, Song, Jian, Zhang, Aijun, Zhang, Xiao, Li, Qingjun, Qu, Xinyan, Wang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718632/
https://www.ncbi.nlm.nih.gov/pubmed/36467981
http://dx.doi.org/10.1155/2022/1748324
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author Zhang, Huimin
Xu, Lin
Song, Jian
Zhang, Aijun
Zhang, Xiao
Li, Qingjun
Qu, Xinyan
Wang, Ping
author_facet Zhang, Huimin
Xu, Lin
Song, Jian
Zhang, Aijun
Zhang, Xiao
Li, Qingjun
Qu, Xinyan
Wang, Ping
author_sort Zhang, Huimin
collection PubMed
description Yinqiao powder, with significant anti‐inflammatory and antiviral effects, is a classical formula for the treatment of febrile diseases in China. During the SARS period in 2003, Yinqiao powder showed a good antipyretic effect. It also plays a major role in the treatment for COVID-19 in China. Although there are many studies on the chemical compositions and pharmacological effects of Yinqiao powder, there are few studies on the quality standard system of it. In our study, a systematic quality evaluation method of Yinqiao powder combining HPLC fingerprint with quantitative analysis of multi-components by single marker (QAMS) based on network pharmacology and UPLC-Q-Exactive-Orbitrap-MS was established for the first time. In the UPLC-Q-Exactive-Orbitrap-MS experiment, a total of 53 compounds were identified in the extract solution of Yinqiao powder. In addition, 33 blood components were characterized, 23 of which were prototypes. The results of network pharmacology analysis showed that Yinqiao powder may inhibit inflammatory responses by suppressing IL-6, CXCL2, TNFα, NF-κB, etc., in the treatment of COVID-19. The HPLC fingerprint analysis of Yinqiao powder was conducted at 237 nm and 29 characteristic peaks were matched, 11 of which were identified. Forsythoside A was selected as the internal standard reference and double-wavelength (237 nm and 327 nm) was established in QAMS experiment. The repeatability was well under different conditions, and the results measured by QAMS were consisted with that of the external standard method (ESM), indicating that the QAMS method was reliable and accurate. The quality evaluation method of Yinqiao powder would be helpful to evaluate the intrinsic quality of Yinqiao powder more comprehensively, which is conducive to improve the quality standard of Yinqiao powder and provide a beneficial guarantee for the clinical treatment of COVID-19.
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spelling pubmed-97186322022-12-03 Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China Zhang, Huimin Xu, Lin Song, Jian Zhang, Aijun Zhang, Xiao Li, Qingjun Qu, Xinyan Wang, Ping J Anal Methods Chem Research Article Yinqiao powder, with significant anti‐inflammatory and antiviral effects, is a classical formula for the treatment of febrile diseases in China. During the SARS period in 2003, Yinqiao powder showed a good antipyretic effect. It also plays a major role in the treatment for COVID-19 in China. Although there are many studies on the chemical compositions and pharmacological effects of Yinqiao powder, there are few studies on the quality standard system of it. In our study, a systematic quality evaluation method of Yinqiao powder combining HPLC fingerprint with quantitative analysis of multi-components by single marker (QAMS) based on network pharmacology and UPLC-Q-Exactive-Orbitrap-MS was established for the first time. In the UPLC-Q-Exactive-Orbitrap-MS experiment, a total of 53 compounds were identified in the extract solution of Yinqiao powder. In addition, 33 blood components were characterized, 23 of which were prototypes. The results of network pharmacology analysis showed that Yinqiao powder may inhibit inflammatory responses by suppressing IL-6, CXCL2, TNFα, NF-κB, etc., in the treatment of COVID-19. The HPLC fingerprint analysis of Yinqiao powder was conducted at 237 nm and 29 characteristic peaks were matched, 11 of which were identified. Forsythoside A was selected as the internal standard reference and double-wavelength (237 nm and 327 nm) was established in QAMS experiment. The repeatability was well under different conditions, and the results measured by QAMS were consisted with that of the external standard method (ESM), indicating that the QAMS method was reliable and accurate. The quality evaluation method of Yinqiao powder would be helpful to evaluate the intrinsic quality of Yinqiao powder more comprehensively, which is conducive to improve the quality standard of Yinqiao powder and provide a beneficial guarantee for the clinical treatment of COVID-19. Hindawi 2022-11-25 /pmc/articles/PMC9718632/ /pubmed/36467981 http://dx.doi.org/10.1155/2022/1748324 Text en Copyright © 2022 Huimin Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Huimin
Xu, Lin
Song, Jian
Zhang, Aijun
Zhang, Xiao
Li, Qingjun
Qu, Xinyan
Wang, Ping
Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title_full Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title_fullStr Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title_full_unstemmed Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title_short Establishment of Quality Evaluation Method for Yinqiao Powder: A Herbal Formula against COVID-19 in China
title_sort establishment of quality evaluation method for yinqiao powder: a herbal formula against covid-19 in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718632/
https://www.ncbi.nlm.nih.gov/pubmed/36467981
http://dx.doi.org/10.1155/2022/1748324
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