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Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression

Most experimental methods for structural biology proceed in vitro and therefore the contribution of the intracellular environment on protein structure and dynamics is absent. Studying proteins at atomic resolution in living mammalian cells has been elusive due to the lack of methodologies. In-cell n...

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Detalles Bibliográficos
Autores principales:	Gerez, Juan A., Prymaczok, Natalia C., Kadavath, Harindranath, Ghosh, Dhiman, Bütikofer, Matthias, Fleischmann, Yanick, Güntert, Peter, Riek, Roland
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718737/ https://www.ncbi.nlm.nih.gov/pubmed/36460747 http://dx.doi.org/10.1038/s42003-022-04251-6

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